权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Trials for the evaluation of hepatic insulin resistance with novel simplified method using platelet

使用血小板的新型简化方法评估肝脏胰岛素抵抗的试验

基本信息

批准号：
12672244
负责人：
MIZUNO Akira
金额：
$ 1.34万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672244/
关键词：
Insulin resistance diabetes mellitus liver cirrhosis portal glucosensor platelet レプチン

项目摘要

We examined insulin sensitivity in 60 patients with chronic liver disease using hyperinsulinemic euglycemic clamp method and our simplified method by platelet aggregation, because we noticed that insulin sensitivity in cirrhotic patients evaluated by simplified method using platelet was tend to be in normal in the other study. So we concluded that simplified method using platelet could reflect insulin resistance only in the peripheral tissues but not in the liver tissue. We found that the C-peptide (CPR) response to oral glucose load during hyperinsulinemic euglycemic clamp (clamp-OGL-CPR method) could evaluate the function of portal glucosensor (PGS). We measured CPR response in 30 diabetics, 10 obese subjects with IGT and 10 obese subjects with normal glucose tolerance and 10 normal subjects as control. The CPR response in the diabetics with or without obesity and obese subjects with IGT was remarkably reduced or completely absent nevertheless that in obese subjects without glucose intolerance (simple obesity) was good. CPR response in the patients with liver cirrhosis during clamp-OGL-CPR method was remarkably reduced like diabetic patient. We supposed that the disorders in PGS might be related to postprandial hyperglycemia not only in diabetics but also in the patients with liver cirrhosis, and could not relate to insulin resistance. Now we have been examining the relation between the function of PGS and the glucose intolerance in the animal model of type 2 diabetes. In other study, we made clear that leptin could increase insulin-stimulated glucose uptake in the peripheral tissues but not in liver tissue.

我们用高胰岛素-正葡萄糖钳夹法和我们的简化血小板聚集法测定了60例慢性肝病患者的胰岛素敏感性。因此，简化的血小板法仅能反映外周组织的胰岛素抵抗，而不能反映肝组织的胰岛素抵抗。我们发现高胰岛素正糖钳夹（钳夹-OGL-CPR方法）期间C肽（CPR）对口服葡萄糖负荷的反应可以评价门静脉葡萄糖传感器（PGS）的功能。本文测定了30例糖尿病患者、10例肥胖IGT患者、10例肥胖糖耐量正常者和10例正常对照者的心肺复苏反应。糖尿病伴或不伴肥胖者和肥胖伴IGT者CPR反应明显降低或完全消失，而肥胖不伴糖耐量异常者（单纯性肥胖）CPR反应良好。肝硬化患者在夹闭-OGL-CPR方法中的CPR反应与糖尿病患者一样明显降低。提示PGS异常可能与餐后高血糖有关，而与胰岛素抵抗无关。本研究在2型糖尿病动物模型中探讨了PGS的功能与糖耐量异常的关系。在其他研究中，我们明确了瘦素可以增加胰岛素刺激的外周组织葡萄糖摄取，但不能增加肝脏组织葡萄糖摄取。