ROLES BIOACTIVE PEPTIDE IN EXERCISE-INDUCED REDISTRIBUTION OF TISSUE BLOOD FLOW OF INTERNAL ORGANS AND MUSCLES

生物活性肽在运动引起的内脏和肌肉组织血流重新分配中的作用

• 批准号: 12680012
• 负责人: IRUKAYAMA-TOMOBE Yoko
• 金额: $ 2.18万
• 依托单位: Department of Sports Medicine, Institute of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680012/
• 关键词: endothelin; endothelin receptor antagonist; redistribution of blood flow; microshere; treadmill; NOx; 慢性運動トレーニング; VO_2max

Endothelin-1 (ET-1) is a vasoconstrictor peptide. Exercise results in redistribution of tissue blood flow, by which blood flow in increased in active muscles, whereas it is decreased in visceral circulation (such as in kidneys and spleen). We previously reported that an intense exercise causes the increase of ET-1 production in the internal organs, then, we hypothesized that ET-1 participates in the exercise-induced redistribution of tissue blood flow. We investigated the effects of acute ET_A receptor blockade on regional tissue blood flow during exercise in rats. Regional blood flow was measured using microsphere technique before and during treadmill running of 30 min duration under pretreatment with ET_A receptor antagonist (TA-0201) or vehicle (both n=7). The decreases in blood flows to the kidney and spleen induced by intense exercise under pretreatment with TA-0201 were significantly less compared with those under pretreatment with TA-0201 were significantly less compared with those under pretreatment with vehicle (kidney : 36.8 ± 7.1 vs. 66.9 ± 5.2 %. p<0.01, spleen : 31.6 ± 6.8 vs. 57.1 ± 9.2 %. p<0.05). Furthermore, the increases in blood flows to the active muscles induced by intense exercise under pretreatment with TA-0201 were significantly less compared with those under pretreatment with vehicle (tibialis anterior muscle : 223.7 ± 36.6 vs. 423.1 ± 83.0 %, p<0.05). The present study suggests that ET-1-mediated vasoconstriction participates in the decrease of blood flow in the kidneys and spleen of rats during exercise, thereby contributing to the increase of blood flow in the active muscles.

内皮素-1（ET-1）是一种血管收缩肽。运动导致组织血流量的重新分配，通过这种重新分配，活动肌肉中的血流量增加，而内脏循环（如肾脏和脾脏）中的血流量减少。我们曾报道剧烈运动可引起内脏器官ET-1的产生增加，因此我们推测ET-1参与了运动引起的组织血流再分配。本实验观察了急性阻断内皮素A受体对运动大鼠局部组织血流量的影响。采用微球技术测定ET_A受体拮抗剂TA-0201或溶剂（n=7）预处理前后运动30 min时局部血流量。与用TA-0201预处理的那些相比，在用TA-0201预处理的情况下由剧烈运动诱导的流向肾脏和脾脏的血流量的减少显著更少，与用媒介物预处理的那些相比显著更少（肾脏：36.8 ± 7.1%对66.9 ± 5.2%）。p<0.01，脾脏：31.6 ± 6.8 vs. 57.1 ± 9.2%。P<0.05）。此外，在用TA-0201预处理下由剧烈运动诱导的活动肌肉的血流量的增加与在用媒介物预处理下的血流量的增加相比显著更少（胫骨前肌：223.7 ± 36.6%对423.1 ± 83.0%，p<0.05）。本研究表明，ET-1介导的血管收缩参与运动过程中大鼠肾脏和脾脏血流量的减少，从而有助于活动肌肉血流量的增加。

项目成果

Yoko I.Tomobe 他5名: "Dietary Docosahexaenoic Acid suppresses inflammation and immunoresponses in contact hypersensitivity reaction in mice."Lipids. 35. 61-69 (2000)

Yoko I. Tomobe 和其他 5 人：“饮食二十二碳六烯酸可抑制小鼠接触性超敏反应中的炎症和免疫反应。”Lipids 35. 61-69 (2000)

Irukayama-Tomobe, Y, et al.: "Dietary Docosahexaenoic Acid suppresses inflammation and immunoresponses in contact hypersensitivity reaction in mice"Lipids. 35. 61-69 (2000)

Irukayama-Tomobe, Y 等人：“膳食二十二碳六烯酸可抑制小鼠接触性超敏反应中的炎症和免疫反应”脂质。

Morizawa, K., Irukayama-Tomobe, Y. et al.: "Dietary oils and phospholipids containing n-3 highly unsaturated fatty acids suppress 2,4-dinitro-1fluorobenzene-induced contact dermatitis in mice"J. Jpn. Oil Chem. Soc.. 49. No.1. 59-65 (2000)

Morizawa, K., Irukayama-Tomobe, Y. 等人：“含有 n-3 高度不饱和脂肪酸的膳食油和磷脂可抑制 2,4-二硝基-1 氟苯诱发的小鼠接触性皮炎”J.

Spyridon Lazaratos, Yoko Irukayama-Tomobe 他3名: "Oxygen radicals mediate the final exacerbation of endothelin-1 -induced gastric ulcer in rat"European Journal of Pharmacology. 413. 121-129 (2001)

Spyridon Lazaratos、Yoko Irukayama-Tomobe 和其他 3 人：“氧自由基介导大鼠内皮素-1 诱导的胃溃疡的最终恶化”欧洲药理学杂志 413. 121-129 (2001)。

Lazaratos, S., Irukayama-Tomobe, Y. et al.: "Oxygen radicals mediate the final exacerbation of endothelin-1-induced gastric ulcer in rat"Eur. J. Pharmacol.. 413. 121-129 (2001)

Lazaratos, S., Irukayama-Tomobe, Y. 等：“氧自由基介导大鼠内皮素-1 诱导的胃溃疡的最终恶化”Eur。