Classification and variation of flavor compounds in traditional tea

本研究的目的是明确阿萨姆邦茶树不同品种鲜叶中儿茶素的组成和含量。Assamica)和中国品种(Camellia sinensis var.)中华草)。以茶叶为原料，经丙酮提取、甲基异丁基酮提取、甲醇溶解等步骤制备儿茶素组分。采用高效液相色谱法对10种天然儿茶素进行了分析。阿萨姆邦品种的总儿茶素、EGCG、心电、EGCgg、ECgg含量均高于中国品种。对日本、韩国、中国、东南亚、斯里兰卡和印度的茶树地方品种叶绿体大亚基基因rbcL的核苷酸序列进行了测定，并对扩增产物进行了直接测序。茶树cpDNA中rbcL基因的核苷酸序列。中华茶树(中国)和茶树(Camellia sinensis var.)Asamica(Assam)。通过假设两个位置的替换来获得比对，位置40(中国的腺嘌呤)和第948位(中国的鸟嘌呤)。茶树rbcL基因的核苷酸序列分析。中华茶树(中国)和茶树(Camellia sinensis var.)Asamica(Assam)的相似性为99.8%。伊拉瓦地茶树和塔里山茶树的rbcL基因的1,307个核苷酸序列与阿萨姆茶相似，只是在rbcL基因的627位有一个不同的碱基。在这个位置，分别观察到胸腺嘧啶和腺嘌呤作为特异碱。

The purpose of the present study is to make clear the composition and content of catechins contained in various kinds of fresh tea leaves from Assam variety (Camellia sinensis var. assamica) and the China variety (Camellia sinensis var. sinensis). Catechin fraction was prepared from tea leaves by acetone extraction, methylisobutylketone extraction and methanol dissolution. Ten naturally occurring catechins were analyzed by High Performance Liquid Chromatography (HPLC). The Assam variety contained larger amounts of total catechins, EGCg, ECg, EGCgg, ECgg than the China variety. The Content of EC was not much different between the Assam variety and China varieties.The nucleotide sequences of the ribulose bisphosphate carboxylase large-subunit (rbcL) gene in chloroplast (cp) DNA were determined on the native varieties of tea cultivated in Japan, Korea, China, Southeast Asia, Sri Lanka and India.Direct sequencing of the amplified cpDNA products were carried out. The nucleotide sequences of the rbcL gene in cpDNA of Camellia sinensis var. sinensis (China) and Camellia sinensis var. assamica (Assam) are presented. Alignments were obtained by assuming two substitutions, at position 40 (adenine in the China) and position 948 (guanine in the China). The nucleotide sequences of the rbcL gene in Camellia sinensis var. sinensis (China) and Camellia sinensis var. assamica (Assam) are 99.8% similar. On the other hand, the 1,307 nucleotide sequences of rbcL of Camellia irrawadiensis and Camellia taliensis were similar sequences to Assam tea, with only a different base at position 627 in the rbcL gene. At this position in C. irrawadiensis and C. taliensis, thymine and adenine were observed, respectively, as specific bases.