Search for target genes of STAT3 that maintains pluripotency of embryonic stem cells

STAT3 is a transcriptional factor that is involved in self-renewal of embryonic stem cells. Toidentify target genes of STAT3 in the self-renewal，we searched for a gene that shows higher expression in undifferentiated ES cells than indifferentiated ES cells by using subtraction method and DNA microarrayand found several candidate genes. Of他们，1. zinc-finger protein (zfp) 57 Oct-3/4，pluripotent stem cell-specific transcription factor，plays a critical role in the self-renewal of ES cells. Several evidences suggest that gene表达式via Oct-3/4 requires an unidentified co-factor(s). We found that Zfp57 is expressedthe self-renewal of ES cells，它的表达式是减少upon differentiation. By co-immunoprecipitation assay，Zfp57 interacts with Oct-3/4. Furthermore，Zfp57 enhanced the transcriptional activity of Oct-3/4 towards the promoter of rex-1，an Oct-3/4 target gene, in humMore an embryonic kidney 293 cells. Our data suggest that Zfp57, a target of STAT3is one of the Oct-3/ 4co -factorsand raise the possibility that this protein may cooperate with Oct-3/4 in induction of Rex-12. Embryonic ectoderm which in turn may play an important role in the self-renewal of ES cells. Embryonic ectodermdevelopment (eed) Recent studies revealed that gene expression is tightly associatedacetylation of histone，histone acetyltransferases and histpne deacylases (HDACs). Bed，a member of the polycomb family这是已知的结合，我们发现之前是一个统计统计的downstream molecules TreatmentES cells with trichostatin A, an HDAC inhibitor, led to the initiation of differentiation，histone acetylation is involved in regulation of the self-renewal. Interestingly，我们发现之前forms a complex also with Rex-1.在常规结果中自定义repression of gene expression，these results suggest the possibility that the Eed Rex-1 HDAC complex may maintain theundifferentiated state of ES cells by suppressing the expression of a differentiation-inducing•(s) . less

STAT3 is a transcriptional factor that is involved in self-renewal of embryonic stem (ES) cells. To identify target genes of STAT3 in the self-renewal, we searched for a gene(s) that shows higher expression in undifferentiated ES cells than in differentiated ES cells by using subtraction method and DNA microarray, and found several candidate genes. Of them, we focused our attention on the following two genes.1. zinc-finger protein (zfp) 57 Oct-3/4, a pluripotent stem cell-specific transcription factor, plays a critical role in the self-renewal of ES cells. Several evidences suggest that gene expression via Oct-3/4 requires an unidentified co-factor(s). We found that Zfp57 is expressed during the self-renewal of ES cells, while its expression is reduced upon differentiation. By co-immunoprecipitation assay, it was also found that Zfp57 interacts with Oct-3/4. Furthermore, Zfp57 enhanced the transcriptional activity of Oct-3/4 towards the promoter of rex-1, an Oct-3/4 target gene, in hum … More an embryonic kidney 293 cells. Our data suggest that Zfp57, a target of STAT3, is one of the Oct-3/4 co-factors, and raise the possibility that this protein may cooperate with Oct-3/4 in induction of Rex-1, which in turn may play an important role in the self-renewal of ES cells.2. Embryonic ectoderm development (eed) Recent studies revealed that gene expression is tightly associated with acetylation of histone, which is controlled by histone acetyltransferases and histpne deacylases (HDACs). Bed, a member of the polycomb family, is known to bind with HDAC. We found that Bed is one of the downstream molecules of STATS. Treatment of ES cells with trichostatin A, an HDAC inhibitor, led to the initiation of differentiation, suggesting that histone acetylation is involved in regulation of the self-renewal. Interestingly, we found that Bed forms a complex also with Rex-1. Since deacylation ofhistone generally results in repression of gene expression, these results suggest the possibility that the Eed・Rex-1・HDAC complex may maintain the undifferentiated state of ES cells by suppressing the expression of a differentiation-inducing gene(s). Less