Structural Analysis of Ethylene (Plant Hormone) Sensor Protein
乙烯(植物激素)传感器蛋白的结构分析
基本信息
- 批准号:12680666
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Ethylene is a plant hormone, which is significantly involved in life cycle of plants. Ethylene exhibits its physiological activities by virtue of binding to its sensor protein, ETR1. ETR1 belongs to the two-component regulatory system in cells, and mainly consists of two functional domain, sensor domain and histidine kinase domain. Most recently, it has been found that the ETR1 sensor domain contains Cu(I). In the present project,we have been trying to obtain the direct evidence that Cu(I) is the ethylene binding site of ETR1. We constructed expression system of the recombinant protein of the ETR1 sensor domain (trans-membrane protein) in E. coli, established it purification procedure, and measured the Cu K-edge XAFS spectra. The spectra is significantly different in the presence and absence of ethylene, showing that ethylene bind to the Cu(I) sitel of ETR1 and causes its coordination structural changes.
乙烯是一种植物激素,与植物的生活史密切相关。乙烯通过与其感受器蛋白ETR1结合而显示其生理活性。ETR1属于细胞内的双组分调控系统,主要由两个功能结构域组成,即感受器结构域和组氨酸激活区。最近,人们发现ETR1传感器结构域中含有铜(I)。在本项目中,我们一直试图获得铜(I)是ETR1的乙烯结合位点的直接证据。我们构建了ETR1感受区重组蛋白(跨膜蛋白)在大肠杆菌中的表达系统,建立了其纯化程序,并测定了其铜K边XAFS光谱。有无乙烯的光谱有明显的不同,表明乙烯与ETR1的铜(I)中心结合并引起其配位结构的变化。
项目成果
期刊论文数量(42)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Kumita, S.Yamada, H.Nakamura, Y.Shiro: "Chimeric sensory kinases containing O2 sensor domain of FixL, and histidine kinase domain from thermophile"Biochem.Biophys.Acta. (印刷中). (2003)
H.Kumita、S.Yamada、H.Nakamura、Y.Shiro:“含有 FixL 的 O2 传感器结构域和来自嗜热菌的组氨酸激酶结构域的嵌合感觉激酶”Biochem.Biophys.Acta(出版中)。
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K. Sakai,Y. Matsui, T. Kouyama, Y. Shiro and S.Adachi: "Optical monitoring of freeze-trapped reaction intermediates in protein crystals: a microspectrophotometer for cryogenic protein crystallography"J. Appl. Cryst. 35. 270-273 (2002)
K.酒井,Y.
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Miyatake,H.,Mukai,M.,Park,S-Y.,Adachi,S.,Tamura,K., et al: "Sensory Mechanism of Oxygen Sensor FixL from Rhizobium meliloti : Crystallographic Mutagenesis and Resonance Raman Spectroscopic Studies"J.Mol.Biol.. 301. 415-431 (2000)
Miyatake,H.、Mukai,M.、Park,S-Y.、Adachi,S.、Tamura,K.等人:“苜蓿根瘤菌氧传感器 FixL 的感觉机制:晶体诱变和共振拉曼光谱研究”J.Mol
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Y.Shiro, Y.Isogai, H.Nakamura, T.Iizuka: "Molecular Anatomy of Cellular System"編者(I.Endo, T.Kudo, H.Osada, T.Shibata, I.Yamaguchi) Elsevier Science B.V.. 231(189-204) (2002)
Y.Shiro、Y.Isogai、H.Nakamura、T.Iizuka:《细胞系统的分子解剖学》编辑(I.Endo、T.Kudo、H.Osada、T.Shibata、I.Yamaguchi)Elsevier Science B.V.. 231 (189-204) (2002)
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R. Makino, E. Obayashi, N. Homma, Y. Shiro and H. Hori: "YC-1 Facilitates Release of the Proximal His Residue in the NO and CO Complexes of Soluble Guanylate Cyclase"J. Biol. Chem.. (in press). (2003)
R. Makino、E. Obayashi、N. Homma、Y. Shiro 和 H. Hori:“YC-1 促进可溶性鸟苷酸环化酶的 NO 和 CO 复合物中近端 His 残基的释放”J。
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SHIRO Yoshitsugu其他文献
SHIRO Yoshitsugu的其他文献
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{{ truncateString('SHIRO Yoshitsugu', 18)}}的其他基金
Molecular Evolution of Respiratory Enzymes; Functional Conversion from NO Reduction to O2 Reduction
呼吸酶的分子进化;
- 批准号:
21245041 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Bio-conversion of Substances and Information by Metal-containing Proteins and Enzymes
含金属蛋白质和酶对物质和信息的生物转化
- 批准号:
16074217 - 财政年份:2004
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Peroxygenase P450 as a Novel Bio-material
过氧化酶 P450 作为一种新型生物材料
- 批准号:
14580616 - 财政年份:2002
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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