Bio-conversion of Substances and Information by Metal-containing Proteins and Enzymes

含金属蛋白质和酶对物质和信息的生物转化

• 批准号: 16074217
• 负责人: SHIRO Yoshitsugu
• 金额: $ 7.42万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16074217/
• 关键词: Metalloproteins; Bio-synthesis; Signal Transduction; Metal-enzymes; coordination Chemistry; ヘム鉄; 二原子酸素添加酵素; 二成分情報伝達系; センサー蛋白質; 一酸化窒素還元酵素; X線結晶構造解析; 配位構造; ヘム; トリプトファン代謝; キヌレニン経路; 老人性白内障; 感染症; 金属配位空間; ヒスチジンキナーゼ; 酵素センサー蛋白質; エチレンセンサー蛋白質

We have examined conversion of bio-substances and information in molecular level by metal-containing enzymes and proteins such as nitric oxide reductase, indoleamine 2, 3-dioxygenase, and oxygen sensor protein. We proposed the reaction mechansim of conversion of NO (nitric oxide) to N20 (nitrous oxide) on the basis of the ESR results of the reaction intermediates trapped by the rapid mixing-to-quench system, and the crystal structure of this enzyme. We also proposed the reaction mechanism of the tryptophan metabolism catalyzed by indoleamine 2,3-dioxygenase on the basis of its crystal structure. We found that the oxygen sensor protein can descrominate 02 from other two-atomic gas molecules such as CO and NO, and also discussed the pathway of the intra- and inter-molecular signal transduction on the basis of the strcuture of the sensor protein of the thermophilic bacterium, which is analogus to the oxygen sensor protein.

我们已经研究了含金属的酶和蛋白质如一氧化氮还原酶、吲哚胺2，3-双加氧酶和氧传感器蛋白质在分子水平上对生物物质和信息的转化。根据快速混合淬灭系统捕获的反应中间体的ESR结果和该酶的晶体结构，提出了NO（nitric oxide）转化为N2 O（nitric oxide）的反应机理。根据吲哚胺2，3-双加氧酶的晶体结构，我们还提出了吲哚胺2，3-双加氧酶催化色氨酸代谢的反应机理。我们发现该氧传感蛋白可以将O2从CO、NO等双原子气体分子中分离出来，并根据嗜热菌氧传感蛋白的结构，讨论了其分子内和分子间的信号转导途径。

项目成果

Resonance Raman Observation of Structural Dynamics of FixL on Signal Transduction and Ligand Discrimination

FixL 结构动力学对信号转导和配体辨别的共振拉曼观察

• 发表时间: 2007
• 期刊: Biochemistry 46
• 作者: Y. Hiruma;et. al.
• 通讯作者: et. al.

酸素添加酵素による物質変換の分子科学

氧化酶物质转化的分子科学

• 发表时间: 2007
• 作者: 城 宜嗣;他3名;城 宜嗣
• 通讯作者: 城 宜嗣

Reaction Mechanism of Heme-containing Oxygenases Based on Their Molecular Structures

基于分子结构的含血红素加氧酶的反应机制

• 发表时间: 2006
• 作者: Shiro Y;Sugimoto H
• 通讯作者: Sugimoto H

ヒト由来Tryptophan 2,3-dioxygenaseの分光学的解析

人源色氨酸 2,3-双加氧酶的光谱分析

• 发表时间: 2007
• 作者: 福村栄維子;杉本宏;永野真吾;瀧尾擴士;井柳尭;小倉尚志;城宜嗣
• 通讯作者: 城宜嗣

酸素添加酵素の分子メカニズム

加氧酶的分子机制

• 发表时间: 2006
• 期刊: 日本結晶学会誌 48
• 作者: 杉本 宏;永野真吾;城 宜嗣
• 通讯作者: 城 宜嗣