Functions of neocortical neurons originating in the ganglionic eminence

起源于神经节隆起的新皮质神经元的功能

• 批准号: 12680731
• 负责人: TAMAMAKI Nobuaki
• 金额: $ 2.37万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680731/
• 关键词: Ganglionic eminence; inhibitory neuron; cell migration; guidance cue; Sema3A; Sema3F; 大脳基低核原基

Basically all the neocortical neurons have been believed to originate in the neocortical ventricular zone. However, the investigator ofthis project found that the GABAergic neurons in the neocortex originate in the ganglionic eminence and migrate to the neocortex at embryonic stages. On the way to migrate from the ganglionic eminence to the neocortex, we could not found any scaffold for the guidance of the tangential cell migration. Instead we found neuropilin-1 and 2 on the Dil labeled tangentially migrating cells. Ligands of neuropilin-1 and -2, Sema3A and 3F were expressed in the primordium of striatum and seemed to repel the neuropilin-1 and -2 positive cells to the neocortex, respectively. In addition, the neuropili-1 positive cells and the neuropilin-2 positive cells were segregated into the IZ and into the cortical plate by Sema3A expression in the VZ and Sema3F expression in the cortical plate, respectively. Neuropilin-1 and Dlx2 positive cells seemed to become the interneurons in the cortical plate. Neuropilin-2 and Dlx2 positive cells seemed to become the pioneer neurons andoligodendrocyte in the IZ. Some of the pioneer neurons seemedto remain in the white matter as white matter neurons which connectneocortices.

基本上所有的新皮质神经元都被认为起源于新皮质心室区。然而，该项目的研究者发现，新皮层中的gaba能神经元起源于神经节隆起，并在胚胎阶段迁移到新皮层。在从神经节隆起向新皮层迁移的过程中，我们找不到任何指导切向细胞迁移的支架。相反，我们在Dil标记的切向迁移细胞上发现了neuropilin-1和2。神经匹林-1和-2、Sema3A和3F的配体在纹状体原基表达，似乎分别排斥神经匹林-1和-2阳性细胞到新皮层。此外，通过VZ中Sema3A和皮质板中Sema3F的表达，分别将neuropili-1阳性细胞和neuropilin-2阳性细胞分离到IZ和皮质板中。Neuropilin-1和Dlx2阳性细胞似乎成为皮质板的中间神经元。Neuropilin-2和Dlx2阳性细胞似乎成为IZ的先驱神经元和少突胶质细胞。一些先驱神经元似乎作为连接新皮层的白质神经元留在了白质中。

项目成果

Furuta T: "In vivo transduction of central neurons using recombinant sindbis virus : Goli-like labeling of dendrites and axons with membrane-targeted fluorescent proteins"J Histochem Cytochem. 49. 1497-1508 (2002)

Furuta T：“使用重组辛德比斯病毒体内转导中枢神经元：用膜靶向荧光蛋白对树突和轴突进行 Goli 样标记”J Histochem Cytochem。

Fujimori,K.,Takeuchi,K.,Yazaki,T.,Uemura,K.,Nojyo,Y.,and Tamamaki,N.: "Expression of L1 and TAG-1 in the corticospinal callosal, and hippocampal commissuralneuron the developing rat telencephalon as revealed by retrograde and in situ hybridization double

Fujimori,K.、Takeuchi,K.、Yazaki,T.、Uemura,K.、Nojyo,Y. 和 Tamamaki,N.：“发育中的大鼠皮质脊髓胼胝体和海马连合神经元中 L1 和 TAG-1 的表达

Tamamaki N: "Evidence that Sema3A and 3F regulate the migration of GABAergic neurons in the developing neocortex"J Comp Neurol. (in press). (2002)

Tamamaki N：“Sema3A 和 3F 调节发育中新皮质中 GABA 能神经元迁移的证据”J Comp Neurol。

Tamamaki,N.,Nakamura,K.,and Kaneko,T.: "Cell migration from the corticostriatal angle to the basal telencephalon in rat embryos."Neuroreport. 12(印刷中). (2000)

Tamamaki, N.、Nakamura, K. 和 Kaneko, T.：“大鼠胚胎中从皮质纹状体角到基底端脑的细胞迁移”（Neuroreport）（2000 年）。

Tamamaki N: "Radial glias and radial fibers : What is the function of radial fibers?"Anatomical Sci. International. 77. 9-17 (2002)

Tamamaki N：“径向胶质细胞和径向纤维：径向纤维的功能是什么？”解剖科学。