Research on the intermediate progenitors of GABAergic neurons found in the subventricular zone of the mouse neocortex

小鼠新皮质脑室下区 GABA 能神经元中间祖细胞的研究

• 批准号: 20300121
• 负责人: TAMAMAKI Nobuaki
• 金额: $ 12.65万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20300121/
• 关键词: 再生医学; 神経科学; 大脳皮質; 神経前駆細胞; 神経細胞; intermediate progenitor; GABA; neocortex; proliferation; array analysis

In the embryonic neocortex, neuronal precursors are generated in the ventricular zone(VZ) and accumulate in the cortical plate. Recently, the subventricular zone(SVZ) of the embryonic neocortex was recognized as an additional neurogenic site for both principal excitatory neurons and GABAergic inhibitory neurons. To gain insight into the neurogenesis of GABAergic neurons in the SVZ, we investigated the characteristics of intermediate progenitors of GABAergic neurons(IPGNs) in mouse neocortex by immunohistochemistry, immunocytochemistry, single-cell RT-PCR, and single-cell array analysis. IPGNs were identified by their expression of some neuronal and cell cycle markers. Moreover, we investigated the origins of the neocortical IPGNs by Cre-loxP fate mapping in transgenic mice and the transduction of part of the telencephalic VZ by Cre-reporter plasmids, and found them in the medial and lateral ganglionic eminence. Therefore they must migrate tangentially within the telencephalon to reach the neocortex. Cell-lineage analysis by simple-retrovirus transduction revealed that the neocortical IPGNs self-renew and give rise to a small number of neocortical GABAergic neurons and a large number of granule and periglomerular cells in the olfactory bulb. IPGNs are maintained in the neocortex and may act as progenitors for adult neurogenesis.

在胚胎的新皮质中，神经元前体在脑室带(VZ)产生并聚集在皮质板中。最近，胚胎新皮质的脑室下区(SVZ)被认为是主要兴奋性神经元和GABA能抑制神经元的附加神经发生部位。为了深入了解SVZ内GABA能神经元的神经发生，我们用免疫组织化学、免疫细胞化学、单细胞RT-PCR和单细胞阵列分析等方法研究了小鼠新皮质内GABA能神经元的中间前体(IPGNs)特征。IPGNs是通过一些神经元和细胞周期标志物的表达来鉴定的。此外，我们还通过Cre-loxP在转基因小鼠中的命运定位以及Cre报告基因对部分端脑VZ的转导来研究新皮质IPGNs的起源，并在内侧和外侧神经节隆起发现了它们。因此，它们必须在端脑内切向迁移，才能到达新皮质。单纯逆转录病毒转导的细胞谱系分析表明，新皮质IPGNs可自我更新，并在嗅球内产生少量的新皮质GABA能神经元和大量的颗粒细胞和球周细胞。IPGNs维持在新皮质中，可能作为成体神经发生的祖细胞。

项目成果

Noradrenergic excitation of a subpopulation of GABAergic cells in the basolateral amygdala via both activation of nonselective cationic conductance and supression of resting K+conductance : A study using GAD67-GFP knock-in mice

通过非选择性阳离子电导的激活和静息 K 电导的抑制来激发基底外侧杏仁核中 GABA 能细胞亚群的去甲肾上腺素能：使用 GAD67-GFP 敲入小鼠的研究

• 发表时间: 2008
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Kaneko K;Tamamaki N;Owada H;Kakizaki T;Kume N;Totsuka M;Yamamoto T;Yawo H;Yagi T;Obata K;Yanagawa Y.
• 通讯作者: Yanagawa Y.

Draxin, a repulsive axon guidance protein, is involved in hippocampal development

• DOI: 10.1016/j.neures.2009.09.1710
• 发表时间: 2010-01-01
• 期刊: NEUROSCIENCE RESEARCH
• 影响因子: 2.9
• 作者: Zhang, Sanbing;Su, Yuhong;Tanaka, Hideaki
• 通讯作者: Tanaka, Hideaki

Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice

• DOI: 10.1016/j.jchemneu.2009.04.003
• 发表时间: 2009-09
• 期刊: Journal of Chemical Neuroanatomy
• 影响因子: 2.8
• 作者: Yayun Wang;Yan‐Yan Wei;Jing Huang;Wei Wang;Nobuaiki Tamamaki;Yun-Qing Li;Sheng-Xi Wu

Excess Salt Causes Cerebral Neuronal Apoptosis and Inflammation in Stroke-Prone Hypertensive Rats Through Angiotensin II-Induced NADPH Oxidase Activation

• DOI: 10.1161/strokeaha.108.517284
• 发表时间: 2008-11-01
• 期刊: STROKE
• 影响因子: 8.3
• 作者: Yamamoto, Eiichiro;Tamamaki, Nobuaki;Kim-Mitsuyama, Shokei
• 通讯作者: Kim-Mitsuyama, Shokei

5-HT3A receptor subunit is expressed in a subpopulation of GABAergic and enkephalinergic neurons in the mouse dorsal spinal cord

5-HT3A 受体亚基在小鼠背脊髓 GABA 能和脑啡肽能神经元亚群中表达

• DOI: 10.1016/j.neulet.2008.04.105
• 发表时间: 2008-08-15
• 期刊: NEUROSCIENCE LETTERS
• 影响因子: 2.5
• 作者: Huang, Jing;Wang, Ya-Yun;Wu, Sheng-Xi
• 通讯作者: Wu, Sheng-Xi