The Effect of the Diet Therapy on Endochondral Ossification in the growing stage

生长期饮食疗法对软骨内骨化的影响

• 批准号: 13307058
• 负责人: KIMURA Mitsutaka
• 金额: $ 20.55万
• 依托单位: Kyushu Dental college
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13307058/
• 关键词: growing stage; remodeling of bone; orthodontic force; labeling method; rat; 小児期; 骨リモデリング; 多重染色; 共焦点レーザー顕微鏡; p-QCT; 歯科矯正力; 顎骨; 2重ラベリング; 骨単位

It is conceivable that the bone formation carried out with the order called absorption-addition-formation after activation, as it is same the absorption and formation of the bone by the movement of a tooth with the orthodontic force. Therefore it was thought that we needed to measure the structure to the evaluation of the remodeling of the bone by the orthodontic force as the bone organization movement. We used the labeling methods with tetracycline and calcein which the nature had the bonding to calcification of bone formation. We used the labeling methods to the juvenile dogs, 6-week and 8-week of whistar rats for experiments. We carried out that the experiment animal were expanded their palate suture by the expansion appliance acted with power of period. Addition, we measured the amount or the quality of bone mineral by undestroyed methods with p-QCT and examined the labeling line of remodeling of bone at the specimen of palate suture under the fluorescence microscope and confocal l … More aser-scanning microscope.From the results, we concluded the following.1:It was able to observe the remodeling of the bone in the juvenile dog with three-dimension by the confocal laser-scanning microscope.2:In the juvenile dog, there were no significantly different between control and experiment group with the speed of osteoid calcification.3:In the juvenile dog, the peak of the speed of osteoid calcification were observed at 2 μm per day in the experiment group.4:It is thought that the 1/2 part between the labial side of rat incisor and the anterior palate foramen, was suited to the observation of the palate suture by the expansion5:In the rat, the speed of calcification was faster the 6-week age rat started expansion than 8-week age. However, there was not effect to the speed by excessive or few amount of the expansion. It was thought that the amount of expansion of 1.5mm was adequate in this study.6:Calcification was advances fairly among the 6-week age when the experiment is started from 6-week age rat.7:It is conceivable that the one that experimented from 6-week ages even in the measurement of cortex bone of bone mineral quantity, cortex bone of bone density and cortex bone cross-section area is easy to calcification. Less

可以想象的是，骨形成在活化后以称为吸收-添加-形成的顺序进行，因为它与通过牙齿在正畸力下的移动而进行的骨的吸收和形成相同。因此，我们认为，我们需要测量的结构，以评价骨改建的正畸力作为骨组织的运动。我们采用四环素和钙黄绿素标记法，这两种标记物的性质与骨形成的钙化有密切的关系。采用标记法对幼犬、6周龄和8周龄的Wistar大鼠进行实验。采用周期性扩张器对实验动物腭部缝进行扩张。另外，我们用p-QCT非破坏性方法测量骨矿含量或质量，并在荧光显微镜和共聚焦显微镜下观察腭缝标本骨重建标记线。 ...更多信息 结果表明：1、共聚焦激光扫描显微镜能三维观察到幼犬的骨改建; 2、幼犬类骨质钙化速度与对照组无明显差异; 3、幼犬类骨质钙化速度峰值出现在实验组每天2 μm处; 4、幼犬切牙唇侧与腭前孔之间的1/2处适合于腭缝的扩张观察; 5、幼犬类骨质钙化速度峰值出现在实验组每天2 μm处。在大鼠中，6周龄开始扩张的钙化速度比8周龄快。而膨胀量的大小对速度的影响不大。认为1.5mm的膨胀量在本研究中是足够的。6：从6周龄开始实验的大鼠，钙化在6周龄之间进行得相当好。7：可以想象，即使在测量皮质骨的骨矿含量、皮质骨的骨密度和皮质骨的横截面积时，从6周龄开始实验的大鼠也容易钙化。少