The Effect of the Diet Therapy on Endochondral Ossification in the growing stage

生长期饮食疗法对软骨内骨化的影响

• 批准号: 13307058
• 负责人: KIMURA Mitsutaka
• 金额: $ 20.55万
• 依托单位: Kyushu Dental college
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13307058/
• 关键词: growing stage; remodeling of bone; orthodontic force; labeling method; rat; 小児期; 骨リモデリング; 多重染色; 共焦点レーザー顕微鏡; p-QCT; 歯科矯正力; 顎骨; 2重ラベリング; 骨単位

It is conceivable that the bone formation carried out with the order called absorption-addition-formation after activation, as it is same the absorption and formation of the bone by the movement of a tooth with the orthodontic force. Therefore it was thought that we needed to measure the structure to the evaluation of the remodeling of the bone by the orthodontic force as the bone organization movement. We used the labeling methods with tetracycline and calcein which the nature had the bonding to calcification of bone formation. We used the labeling methods to the juvenile dogs, 6-week and 8-week of whistar rats for experiments. We carried out that the experiment animal were expanded their palate suture by the expansion appliance acted with power of period. Addition, we measured the amount or the quality of bone mineral by undestroyed methods with p-QCT and examined the labeling line of remodeling of bone at the specimen of palate suture under the fluorescence microscope and confocal l … More aser-scanning microscope.From the results, we concluded the following.1:It was able to observe the remodeling of the bone in the juvenile dog with three-dimension by the confocal laser-scanning microscope.2:In the juvenile dog, there were no significantly different between control and experiment group with the speed of osteoid calcification.3:In the juvenile dog, the peak of the speed of osteoid calcification were observed at 2 μm per day in the experiment group.4:It is thought that the 1/2 part between the labial side of rat incisor and the anterior palate foramen, was suited to the observation of the palate suture by the expansion5:In the rat, the speed of calcification was faster the 6-week age rat started expansion than 8-week age. However, there was not effect to the speed by excessive or few amount of the expansion. It was thought that the amount of expansion of 1.5mm was adequate in this study.6:Calcification was advances fairly among the 6-week age when the experiment is started from 6-week age rat.7:It is conceivable that the one that experimented from 6-week ages even in the measurement of cortex bone of bone mineral quantity, cortex bone of bone density and cortex bone cross-section area is easy to calcification. Less

可以想象，活化后的骨形成是按照吸收-添加-形成的顺序进行的，这与牙齿在正畸力的作用下移动骨的吸收和形成是一样的。因此，人们认为，我们需要测量结构来评估正畸力作为骨组织运动对骨的重塑。我们采用了四环素和钙黄绿素的标记方法，它们的性质与骨形成的钙化有结合。我们利用标记方法对幼犬、6周龄和8周龄的白鼠进行实验。我们通过周期动力作用的扩张器对实验动物进行了腭缝扩张。另外，我们用p-QCT以未破坏的方式测量了骨矿物质的量或质量，并在荧光显微镜和共焦激光扫描显微镜下检查了腭缝标本上骨重塑的标记线。从结果中，我们得出以下结论：1：能够通过三维观察幼犬骨的重塑标记线。 2：在幼犬中，对照组和实验组之间骨样钙化速度无显着差异。3：在幼犬中，实验组在每天2μm处观察到骨样钙化速度峰值。4：认为大鼠门牙唇侧与前腭孔之间的1/2部分适合 扩张后腭缝的观察5：在大鼠中，6周龄开始扩张的大鼠钙化速度比8周龄大鼠更快。然而，膨胀量过多或过少对速度没有影响。本研究认为1.5mm的扩张量就足够了。 6：从6周龄大鼠开始实验时，钙化在6周龄期间相当进展。 7：可以想象，即使在皮质骨骨矿物质量、皮质骨骨密度和皮质骨横截面积的测量中，从6周龄开始进行实验的大鼠也很容易钙化。较少的