Ion mobility mass spectrometer with electrospray ionisation (ESI-IMS-MS) for protein analysis

科隆大学新成立的植物代谢生物化学实验室结合了质谱学和生物化学方法，在环境相互作用的背景下研究植物的代谢过程，重点是蛋白质和蛋白质组分析。植物代谢生物化学系是植物科学英才小组(CEPLAS)的一部分，将与该小组的其他小组合作，为各种项目提供蛋白质组学方法。新的研究项目需要拟议的电喷雾离子迁移质谱仪(ESI-IMS-MS)，并在新的地点继续运行项目。植物与其生物和非生物环境相互作用中的信号转导过程是人们研究的重点。为了可靠地检测和量化蛋白质丰度的动态，蛋白质与其他蛋白质或配体的相互作用，以及个别蛋白质的翻译后修饰，需要高分辨率的高灵敏度质谱仪。除了疏水性分离(HPLC)、质荷比(m/z)分离(四极)和质量分析(离子捕捉器/飞行管)外，IMS质谱仪系统还增加了一个分析离子迁移率的模块，并将其用作进一步的分离标准。这种第四维的分离大大增加了分析的多肽的数量，从而在识别的蛋白质数量和定量的准确性方面都实现了显着的增加。此外，还需要IMS模块，以便能够根据分子的三维结构分离相同质量的分子。主要申请者的工作组以前设在汉诺威莱布尼茨大学植物蛋白质组学系。该部门有三台用于蛋白质组分析的质谱仪，使小组成员能够获得实践经验，并发展植物蛋白质组学的广泛专业知识。

The newly established plant metabolic biochemistry lab at the University of Cologne uses a combination of mass spectrometry and biochemical approaches to study plant metabolic processes in the context of environmental interactions with a focus on protein and proteome analyses. The Department of Plant Metabolic Biochemistry is part of the Cluster of Excellence on Plant Sciences (CEPLAS) and will contribute proteomic approaches to various projects in cooperation with other groups of the cluster. The proposed ion mobility mass spectrometer with electrospray ionization (ESI-IMS-MS) is required for new research projects and to continue running projects at the new location. Signaling processes in the interaction of plants with their biotic and abiotic environment are the focus of research interest. In order to reliably detect and quantify the dynamics of protein abundances, the interaction of proteins with other proteins or ligands, as well as post-translational modifications at individual proteins, a highly sensitive mass spectrometer with a high resolution is required. In addition to separation by hydrophobicity (HPLC), separation by mass-to-charge (m/z) ratio (quadrupole) and mass analysis (ion trap/flight tube), IMS mass spectrometry systems have an additional module that analyses ion mobility and uses it as a further separation criterion. This fourth dimension of separation significantly increases the number of peptides analyzed and thus achieves a dramatic increase in both the number of proteins identified and the accuracy of quantification. The IMS module is furthermore required to enable the separation of molecules of identical mass based on their three-dimensional structure. The lead applicant's working group was previously based in the Department of Plant Proteomics at Leibniz Universität Hannover. Three mass spectrometers for proteome analysis were available in the department allowing the group members to gain hands-on experience and develop a broad expertise for plant proteomics.