An ion mobility-mass spectrometry based platform for structural proteomics

基于离子淌度-质谱的结构蛋白质组学平台

• 批准号: LE240100135
• 负责人: Prof Tara Pukala
• 金额: $ 58.68万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2024
• 资助国家: 澳大利亚
• 起止时间: 2024-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE240100135
• 关键词: ion; mobility; mass; spectrometry; based

This project aims to establish a nationally unique facility dedicated to structural proteomics, combining high resolution ion mobility mass spectrometry with advanced separation, hydrogen/deuterium exchange and imaging platforms. Such technology is critical to characterise 3D biomacromolecular structures, dynamics, interactions and spatial location on a proteome-wide scale, and overcome current analytical limitations for structure determination from complex biological samples, particularly for closely related (isomeric) components. Servicing a diverse research community, this will enable new molecular insights to better understand the natural world, and accelerate cutting edge biotechnology advances intersecting life and chemical sciences.

该项目旨在建立一个致力于结构蛋白质组学的全国独一无二的设施，将高分辨率离子迁移质谱仪与先进的分离、氢/氢交换和成像平台相结合。这种技术对于在蛋白质组范围内表征三维生物大分子结构、动力学、相互作用和空间位置至关重要，并克服了目前从复杂生物样品中确定结构的分析限制，特别是对密切相关的(同分异构体)组分。这将为多样化的研究社区提供服务，使新的分子洞察力能够更好地了解自然世界，并加速生命科学和化学科学交叉的尖端生物技术进步。