General study of development of periodontal disease examination

牙周病检查发展概况研究

• 批准号: 13357018
• 负责人: KAWAGUCHI Hiroyuki
• 金额: $ 31.45万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13357018/
• 关键词: Periodontal Disease; Diagnosis; Screening; Preiodontal Pathogen; PCR method; cytokine

We developed a new method to detect periodontal pathogens. PCR method is very useful for making periodontal treatment plan and decision of prognosis. DNA microarray is also useful to know state of periodontal disease. On the other hand, we developed useful diagnosis method for disease sensitivity. Fc γ receptor gene regulates the production of inflammatory cytokines. Fc γ receptor polymorphisms affect the host response to bacterial attack. Profile analysis of inflammatory related factors from tissues, saliva, or gingival crevicular fluids (GCF) is available for the development of new therapy. Glycosaminoglycan level in GCF correlated with alveolar bone resorption. The measurement of calprotectin from GCF is associated with the activity of periodonttitis. Moreover we identified that volatile sulfer compound of expiration is closely correlated with periodontal disease. We developed a new method to detect traumatic occlusion using tooth fixation. To examine the effect of periodontitis for the body, we identified that high sensitivity CRP is very useful.

我们开发了一种新的方法来检测牙周病原体。PCR方法对牙周治疗方案的制定和预后判断有重要意义。DNA微阵列也有助于了解牙周病的状态。另一方面，我们开发了有用的诊断方法的疾病敏感性。Fc γ受体基因调控炎性细胞因子的产生。Fc γ受体多态性影响宿主对细菌攻击的反应。对组织、唾液或龈沟液（GCF）中的炎症相关因子进行谱分析可用于开发新的治疗方法。GCF中糖胺聚糖水平与牙槽骨吸收相关。GCF中钙卫蛋白的测定与牙周炎的活动性相关。此外，我们还发现呼气中的挥发性硫化物与牙周病密切相关。我们开发了一种新的方法来检测创伤咬合使用牙齿固定。为了检查牙周炎对身体的影响，我们确定了高灵敏度CRP是非常有用的。

项目成果

Sakamoto M, Umeda M, et al.: "Reclassification of Bacteroides forsythus as Tanerrela forsythensis corrig"gen.Nov.comb.Nov.Int J.Syst Evol Microbiol. 52. 841-849 (2002)

Sakamoto M、Umeda M 等人：“福赛拟杆菌重新分类为 Tanerrela forsythensis corrig”gen.Nov.comb.Nov.Int J.Syst Evol Microbiol。

Setoguchi T., Izumi Y., and et al.: "The effects of methyl-mercoptan on epithelial cell growth and proliferation"International Dental Journal. 52. 241-246 (2002)

Setoguchi T.、Izumi Y. 等人：“甲硫醇对上皮细胞生长和增殖的影响”国际牙科杂志。

Nishimura F., and et al.: "Periodontal disease and diabetes mellitus : the role of tumor nectosis factor-α in a 2-way relationship"J. Periodontal. 74. 97-102 (2003)

Nishimura F. 等人：“牙周疾病和糖尿病：肿瘤坏死因子-α 在双向关系中的作用”J.牙周病杂志 74. 97-102 (2003)

Setoguchi T, Izumi Y, et al.: "The effects of methylmercaptan an epithelial cell gromth and proliferation"International Dental Journal. 52. 241-246 (2002)

Setoguchi T、Izumi Y 等人：“甲硫醇对上皮细胞生长和增殖的影响”国际牙科杂志。

Oshima M, et al.: "Hepatocyte growth factor in saliva : HGF activator expression by gingival epithelial cells"J Oral Sci. 314. 129-134 (2002)

Oshima M 等人：“唾液中的肝细胞生长因子：牙龈上皮细胞的 HGF 激活剂表达”J Oral Sci。