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The better isolation and detection methods of cranial neural crest stem cells (CNCSC) and the therapeutic potentiality of CNCSC in the treatment of craniofacial abnormalities.

颅神经嵴干细胞（CNCSC）更好的分离和检测方法以及CNCSC在治疗颅面异常方面的治疗潜力。

基本信息

批准号：
13357015
负责人：
ETO Kazuhiro
金额：
$ 32.03万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

项目摘要

We tried isolation and isolation of Cranial Neural Crest Stem Cells for the cell-based therapy in the future indicated as below.1)Identification of the stem cell niche of Neural Crest Cellsa)Distribution pattern of Cranial Neural Crest Cells during Craniofacial morphogenesis in Wnt-1/Cre mouse.The distribution pattern of Cranial Neural Crest Cells (CNCCs) was examined in Wnt-1/Cre mouse, which indicate b-galactosidase activity in Neural Crest Cells, during craniofacial morphogenesis. B-galactosidase positive CNCCs were identified in the frontal and facial bone, and these pattern were constructed until E9.5.b)Method for identification of Mesenchymal Stem cells in Craniofacial region by chasing the Label-Retaining Cells (LRCs).2)Isolation of Cranial Neural Crest Stem Cells (CNCSCs).a)Modification the isolation methods of CNCSCs3)Molecular understanding of cell proliferation and apoptosis in cranial neural crest cellsa)Expression pattern of cell-cycle regulators in developing cranial sutu … More reb)Cell cycle regulation in terminal-differentiated cells inc)Twist-dependent regulation of cell proliferation and apoptosisWe examined Twist function in CNCCs in Twist null mice and found that Twist is essential for the Cranial nerve patterning, cell growth, and anti-apoptotic activity in CNCCs. We also developed the knockdown methods of Twist activity in vitro by DNazyme and, antisense morpholino nucleotide methods.d)The transcriptional regulation of cell-cycle regulator E2F1.e)Cell-cycle related acetylation of the p220/NPAT by CBP histone acetyltransferase4)Clinical potentialities of Cranial Neural Crest Cellsa)Periodontal tissue regeneration by in situ tissue engineering by the combination of growth factors and scaffoldsb)Periodontal tissue regeneration by in situ tissue engineering by the combination of cell and scaffoldsWe examined the clinical potentialities of cranial neural crest cells by tissue engineering methods, and data suggested that they will be a powerful tool in cell-based therapy in the future.5)Gene expression profiling of the Cranial Neural Crest Stem Cells Less

我们尝试分离和分离颅神经嵴干细胞用于未来的细胞治疗，如下所示。1)神经嵴细胞干细胞生态位的确定(1)Wnt-1/Cre小鼠颅面形态发生过程中颅神经嵴细胞的分布规律。研究了Wnt-1/Cre小鼠颅面形态形成过程中颅神经嵴细胞（CNCCs）的分布规律，表明神经嵴细胞中b-半乳糖苷酶活性。b -半乳糖苷酶阳性cncc出现在额骨和面骨，这种模式持续到E9.5。b)追踪标记保留细胞（label - preserving cells, lrc）鉴定颅面间充质干细胞的方法。2)颅神经嵴干细胞（CNCSCs）的分离。a) cncscs分离方法的改进3)颅神经嵴细胞增殖和凋亡的分子机制3)颅神经嵴细胞中细胞周期调控因子的表达模式更多reb)末梢分化细胞的细胞周期调控包括Twist依赖性细胞增殖和凋亡的调控我们在Twist阴性小鼠中检测了cncscs的Twist功能，发现Twist对脑神经的模式化、细胞生长和细胞凋亡至关重要。和抗凋亡活性。我们还开发了DNazyme法和反义morpholino核苷酸法在体外敲除Twist活性的方法。d)细胞周期调控因子E2F1的转录调控。e) CBP组蛋白乙酰转移酶对p220/NPAT细胞周期相关乙酰化的影响4)生长因子与支架结合原位组织工程再生牙周组织的临床潜力b)细胞与支架结合原位组织工程再生牙周组织我们考察了组织工程方法培养颅神经嵴细胞的临床潜力。数据表明，它们将成为未来细胞疗法的有力工具。5)颅神经嵴干细胞基因表达谱分析

项目成果

期刊论文数量（43）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of the curly tail genotype on neuroepithelial integrity and cell proliferation during late stages of primary neurulation.

卷尾基因型对初级神经形成后期神经上皮完整性和细胞增殖的影响。

DOI：
发表时间：
2001
期刊：
J.Anat 199
影响因子：
0
作者：
Hall M.;Gofflot F.;Iseki S.;Morriss-Kay G.M.
通讯作者：
Morriss-Kay G.M.

Twist is involved in establishment of the coronal suture.

扭转涉及冠状缝的建立。

DOI：
发表时间：
期刊：
J.Anatomy In press
影响因子：
0
作者：
Yoshida T.;Phylactou LA.;Uney JB.;Ishikawa I.;Eto K.;Iseki S.
通讯作者：
Iseki S.

Kubota K, Iseki S, Kuroda S, Oida S, Iimura T, Duarte WR, Ohya K, Ishikawa I, Kasugai S.: "Synergistic effect of fibroblast growth factor-4 in ectopic bone formation in-duced by bone morphogenetic protein-2"Bone. 31. 465-471 (2002)

Kubota K、Iseki S、Kuroda S、Oida S、Iimura T、Duarte WR、Ohya K、Ishikawa I、Kasugai S.：“成纤维细胞生长因子 4 在骨形态发生蛋白 2 诱导的异位骨形成中的协同作用

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Expression of p16(INK4a) in tumors.

p16(INK4a)在肿瘤中的表达。

DOI：
发表时间：
2003
期刊：
Oral Oncology. 9
影响因子：
0
作者：
Auerkari E.I.;Suwelo I.S.;Tjarta A.;Cornain S.;Sarwono A.T.;Widurini;Joewono;V;Handjari;D.R.;Eto;K.;Ikeda;M.A.
通讯作者：
M.A.

Development and cell dynamics of PLCb2 cells in mouse taste buds.

小鼠味蕾中 PLCb2 细胞的发育和细胞动力学。