The better isolation and detection methods of cranial neural crest stem cells (CNCSC) and the therapeutic potentiality of CNCSC in the treatment of craniofacial abnormalities.

我们尝试分离和分离脑神经嵴干细胞，以用于以下所示的未来基于细胞的治疗：1）神经嵴细胞的干细胞生态位的鉴定a）Wnt-1/Cre小鼠颅面形态发生过程中脑神经嵴细胞的分布模式检测了Wnt-1/Cre小鼠中脑神经嵴细胞（CNCC）的分布模式，其表明在颅面形态发生期间神经嵴细胞中的β-半乳糖苷酶活性。在额骨和面骨中鉴定出B-半乳糖苷酶阳性CNCC，b）通过追踪标记保留细胞（LRC）鉴定颅面区域中的间充质干细胞的方法2）颅神经嵴干细胞（CNCSC）的分离a）CNCSC分离方法的改进3）颅神经嵴细胞中细胞增殖和凋亡的分子理解a）颅缝发育过程中细胞周期调控因子的表达 ...更多信息 reb）终末分化细胞中的细胞周期调节inc）细胞增殖和凋亡的Twist依赖性调节我们检测了Twist缺陷小鼠CNCC中的Twist功能，发现Twist对于CNCC中的颅神经图案形成、细胞生长和抗凋亡活性是必需的。我们还开发了DNazyme体外敲除Twist活性的方法，反义吗啉代核苷酸方法。d）细胞周期调节因子E2 F1的转录调节。e）CBP组蛋白乙酰转移酶对p220/NPAT的细胞周期相关乙酰化。4）颅神经嵴细胞的临床潜力。a）通过生长因子和支架组合的原位组织工程进行的牙周组织再生。b）细胞与支架结合原位组织工程牙周组织再生我们通过组织工程方法检测了颅神经嵴细胞的临床潜力，数据表明它们将成为未来细胞治疗的有力工具。5）颅神经嵴干细胞的基因表达谱研究

We tried isolation and isolation of Cranial Neural Crest Stem Cells for the cell-based therapy in the future indicated as below.1)Identification of the stem cell niche of Neural Crest Cellsa)Distribution pattern of Cranial Neural Crest Cells during Craniofacial morphogenesis in Wnt-1/Cre mouse.The distribution pattern of Cranial Neural Crest Cells (CNCCs) was examined in Wnt-1/Cre mouse, which indicate b-galactosidase activity in Neural Crest Cells, during craniofacial morphogenesis. B-galactosidase positive CNCCs were identified in the frontal and facial bone, and these pattern were constructed until E9.5.b)Method for identification of Mesenchymal Stem cells in Craniofacial region by chasing the Label-Retaining Cells (LRCs).2)Isolation of Cranial Neural Crest Stem Cells (CNCSCs).a)Modification the isolation methods of CNCSCs3)Molecular understanding of cell proliferation and apoptosis in cranial neural crest cellsa)Expression pattern of cell-cycle regulators in developing cranial sutu … More reb)Cell cycle regulation in terminal-differentiated cells inc)Twist-dependent regulation of cell proliferation and apoptosisWe examined Twist function in CNCCs in Twist null mice and found that Twist is essential for the Cranial nerve patterning, cell growth, and anti-apoptotic activity in CNCCs. We also developed the knockdown methods of Twist activity in vitro by DNazyme and, antisense morpholino nucleotide methods.d)The transcriptional regulation of cell-cycle regulator E2F1.e)Cell-cycle related acetylation of the p220/NPAT by CBP histone acetyltransferase4)Clinical potentialities of Cranial Neural Crest Cellsa)Periodontal tissue regeneration by in situ tissue engineering by the combination of growth factors and scaffoldsb)Periodontal tissue regeneration by in situ tissue engineering by the combination of cell and scaffoldsWe examined the clinical potentialities of cranial neural crest cells by tissue engineering methods, and data suggested that they will be a powerful tool in cell-based therapy in the future.5)Gene expression profiling of the Cranial Neural Crest Stem Cells Less