The better isolation and detection methods of cranial neural crest stem cells (CNCSC) and the therapeutic potentiality of CNCSC in the treatment of craniofacial abnormalities.

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We tried isolation and isolation of Cranial Neural Crest Stem Cells for the cell-based therapy in the future indicated as below.1)Identification of the stem cell niche of Neural Crest Cellsa)Distribution pattern of Cranial Neural Crest Cells during Craniofacial morphogenesis in Wnt-1/Cre mouse.The distribution pattern of Cranial Neural Crest Cells (CNCCs) was examined in Wnt-1/Cre mouse, which indicate b-galactosidase activity in Neural Crest Cells, during craniofacial morphogenesis. B-galactosidase positive CNCCs were identified in the frontal and facial bone, and these pattern were constructed until E9.5.b)Method for identification of Mesenchymal Stem cells in Craniofacial region by chasing the Label-Retaining Cells (LRCs).2)Isolation of Cranial Neural Crest Stem Cells (CNCSCs).a)Modification the isolation methods of CNCSCs3)Molecular understanding of cell proliferation and apoptosis in cranial neural crest cellsa)Expression pattern of cell-cycle regulators in developing cranial sutu … More reb)Cell cycle regulation in terminal-differentiated cells inc)Twist-dependent regulation of cell proliferation and apoptosisWe examined Twist function in CNCCs in Twist null mice and found that Twist is essential for the Cranial nerve patterning, cell growth, and anti-apoptotic activity in CNCCs. We also developed the knockdown methods of Twist activity in vitro by DNazyme and, antisense morpholino nucleotide methods.d)The transcriptional regulation of cell-cycle regulator E2F1.e)Cell-cycle related acetylation of the p220/NPAT by CBP histone acetyltransferase4)Clinical potentialities of Cranial Neural Crest Cellsa)Periodontal tissue regeneration by in situ tissue engineering by the combination of growth factors and scaffoldsb)Periodontal tissue regeneration by in situ tissue engineering by the combination of cell and scaffoldsWe examined the clinical potentialities of cranial neural crest cells by tissue engineering methods, and data suggested that they will be a powerful tool in cell-based therapy in the future.5)Gene expression profiling of the Cranial Neural Crest Stem Cells Less