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Investigation of organaizers in vertebrate craniofacial development

脊椎动物颅面发育组织者的调查

基本信息

批准号：
10307043
负责人：
ETO Kazuhiro
金额：
$ 26.3万
依托单位：
Tokyo Medical and Dental University, Graduate School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

Craniofacial development of vertebrates involves several tissue components of early stage embryos, ectoderm, neural tube, cranial neural crest cells, mesodem, foregut endoderm. To investigate the mechanism of craniofacial development provides us with not only understanding of craniofacial anomalies but also basic data for craniofacial reconstitution. Our current research results have suggested that the initiation and development of head organs requires spatio-temporal localization and interaction of these tissues:1 ) mandible arch formation requires the interaction between the oral ectoderm and posterior midbrain crest cell-derived mesenchyme at the proximooral part of the first pharyngeal arch;2) tooth bud formation begins where oral ectoderm, foregut endoderm and neural crest cells meet;3) Rathke's pouch is derived from the ectoderm which locates just anterior to the rostral part of the neural plate and later composes the stomodeal roof with foregut endoderm;4) mouse coronal and sagittal sutures are formed at a neural crest-mesoderm interface, and the initiation of skull vault bone osteogenesis coincides with the dynamic growth of cerebral hemispheres caudally. This supports the idea of organizing center that tissue boundary generates positional information for organogenesis, proposed by Meinhardt. Based on these results we are planning to clarify the mechanism of the interaction, key molecules of the events and regulation of their expression.

脊椎动物颅面发育涉及早期胚胎、外胚层、神经管、颅神经嵴细胞、中胚层、前肠内胚层等组织成分。探讨颅面发育的机制，不仅有助于认识颅面畸形，而且为颅面重建提供基础资料。我们目前的研究结果表明，头部器官的发生和发育需要这些组织的时空定位和相互作用：1)下颌骨弓的形成需要口腔外胚层和后中脑嵴细胞来源的间质在第一咽弓近口部的相互作用；2)牙芽形成始于口腔外胚层、前肠内胚层和神经嵴细胞的交汇处；(3) Rathke's pouch来源于外胚层，位于神经板吻侧前部，后与前肠内胚层组成气孔顶；4)小鼠的冠状和矢状缝合线形成于神经嵴-中胚层交界面，颅骨穹窿骨成骨的起始与脑半球尾部的动态生长一致。这支持了Meinhardt提出的组织中心的观点，即组织边界为器官发生产生位置信息。基于这些结果，我们计划阐明相互作用的机制、事件的关键分子及其表达调控。