Application of endogenous autofluorescence signal for functional brain imaging

内源性自发荧光信号在功能性脑成像中的应用

• 批准号: 13358013
• 负责人: SHIBUKI Katsuei
• 金额: $ 32.53万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13358013/
• 关键词: Flavoprotein; Autofluorescence; Functional brain imaging; Associative learning; Mouse; Auditory cortex; Somatosensory cortex; Visual cortex; フラビン蛋白質; 光学的解析法; 脳切片; 大脳皮質

We developed a method of neural imaging using activity-dependent changes in green fluorescence of flavoproteins excited by blue light. We observed an increase in endogenous green fluorescence in response to electrical stimulation in the slices of the rat auditory cortex. The amplitude of the peak increase, which was observed 2-3 s after the initiation of the repetitive electrical stimulation, was as large as about 30% at room temperature. They were also not observed in the slices perfused with medium lacking calcium, glucose or oxygen. These data indicate that the green fluorescent responses are attributed to the fluorescence of flavoproteins involved in the energy metabolism in mitochondria. In anesthetized rats with urethane, we observed clear images of the green fluorescent changes on the exposed surface of the auditory cortex following sound stimuli. These fluorescent changes were observed without using averaging, since they were much larger than the optical noises caused by heartb … More eats or breathing. We also tried to investigate spatial pattern of correlation between spontaneous neural activites using fluctuations in flavoprotein fluorescence. In the normal somatosensory cortex covered with agar, positive correlation around a ROI was observed in the fluorescence signals after correction for breathing movements. Tetanic stimulation applied around the ROI increased the magnitude of the positive correlation around the ROI. This method was also used to visualize learning-induced changes of neural activities in the rat primary somatosensory cortex. A water-deprived rat was trained to discriminate the frequency (20 Hz or 40 z) of floor vibration in a Skinner box. After this discrimination learning was achieved in consecutive 3-5 days, the somatosensory neural responses to the rewarded frequency were larger than that in response to the unrewarded frequency in all of the rats tested. In mice, the transparent skull of mice allowed trans-cranial autofluorescence imaging of cortical activites. Less

项目成果

関 健二朗 他2名: "Sequence dependence of posttetanic potentiation after sequential heterosynaptic stimulation in the rat auditory cortex."Journal of Physiology (London). 533. 503-518 (2001)

Kenjiro Seki 和其他 2 人：“大鼠听觉皮层连续异质突触刺激后强直后增强的序列依赖性。”生理学杂志（伦敦）533. 503-518 (2001)。

Murakami H, Kamatani D, Hishida R, Takao T, Kudoh M, Kawaguchi T, Tanaka R, Shibuki K: "Short-term plasticity visualized with flavoprotein autofluorescence in the somatosensory cortex of anesthetized rats."Eur J Neurosci. 19. 1352-1360 (2004)

Murakami H、Kamatani D、Hishida R、Takao T、Kudoh M、Kawaguchi T、Tanaka R、Shibuki K：“麻醉大鼠体感皮层黄素蛋白自发荧光可视化的短期可塑性。”Eur J Neurosci。

菱田竜一他4名: "Anisotrapic functional connections between the auclitory cortex and area18a in rat cerebral slices"Neuroscience Research. (in press). (2003)

Ryuichi Hishida 和其他 4 人：“大鼠大脑切片中听觉皮层和区域 18a 之间的各向异性功能连接”神经科学研究（2003 年）。

関健二郎 他2名: "Polysynaptic slow depolarization and spiking activity elicited after induction of long-term potentiation in rat auditory cortex"Brain Research. 988. 114-120 (2003)

Kenjiro Seki 和其他 2 人：“在大鼠听觉皮层诱导长期增强后引发多突触缓慢去极化和尖峰活动”《大脑研究》988. 114-120 (2003)。

Seki K, Kudoh M, Shibuki K: "Polysynaptic slow depolarization and spiking activity elicited after induction of long-term potentiation in rat auditory cortex."Brain Res.. 988. 114-120 (2003)

Seki K、Kudoh M、Shibuki K：“在大鼠听觉皮层诱导长期增强后引发多突触缓慢去极化和尖峰活动。”Brain Res.. 988. 114-120 (2003)