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Analysis of GLP (germin-like protein) genes related to photoperiodic response

与光周期响应相关的GLP（胚芽样蛋白）基因分析

基本信息

批准号：
13640654
负责人：
ONO Michiyuki
金额：
$ 2.18万
依托单位：
University ofTsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640654/
关键词：
Ardbidopsis circadian rhythm day-length floral induction flowering germin-like protein Pharbitis nil photoperiodism

项目摘要

PnGLP (Pharbitis nil gemin-like protein) is a gene whose transcript is accumulated in leaves and cotyledons of the very sensitive short-day plant, P. nil cv. Violet during the flower-inductive dark-period and encodes a member of the leaf-type subfamily of GLP genes. The functions of GLP proteins are mostly unkown. Northern analysis revealed that the level of PnGLP mRNA showed a circadian rhythm, with maxima around 10 h after the beginning of the dark period, and this oscillation continued in lengthened continuous daikness. Homologues of PnGLP in Aratidcpsis (along-day plait), namely, AtGLP1 and AtGLP2 were isolated and characterized Accumulation of AtGLP1 and AtGLP2 mRNA in leafalso showed circadian rhythms, with maxima around 14 h after the beginning of the light period. These oscillaticns continued mostly under continuous light. The mode of regulation in AtGLP1 and AtGLP2 showed opposite to that of PnGLP, possibly reflecting the differences between short-day plants and long-day plants. To study the mdecularmechanisms ofleaf-type GLP gsne regulation in relati on to photoperiodism, we decided to analyze promoter region ofthese genes using a luciferase gene as a reporter. Because there were no conserved cis-lements among these ptomoteis, we are currently analyzing a series of promoter deletion constructs to identify which sequence motif is essential for circadian rhythmic expression. To deteimine the effects of PnGLP to photoperiodic induction of flowering. we are cumently studying tiansgenic plants expressing PnGLP cDNA. We used short-day tobacco plant (Nicotiona tabacum cv. Maryland Mammoth) as a host plant Tobacco plants that express PnGLP cDNA were likely to form flower earHerwhen they compared to control plant. Tobacco plants that express PnGLP cDNA were likely to from works as a mild stimulator for photoperiodic induction of flowering in short-day plants. Our experiment is currently underway on such hypothesis.

PnGLP（Pharbitis nil gemin 样蛋白）是一种基因，其转录本积累在非常敏感的短日照植物 P. nil cv. 的叶子和子叶中。花诱导黑暗期期间呈紫色，编码 GLP 基因叶型亚家族的成员。 GLP 蛋白的功能大多是未知的。 Northern分析显示，PnGLP mRNA水平呈现昼夜节律，在黑暗期开始后10小时左右达到最大值，并且这种振荡在延长的连续白日中持续存在。分离并表征了 Aratidcpsis（长日辫）中 PnGLP 的同源物 AtGLP1 和 AtGLP2。AtGLP1 和 AtGLP2 mRNA 在叶子中的积累也表现出昼夜节律，在光照期开始后 14 小时左右达到最大值。这些振荡大部分在连续光下持续存在。 AtGLP1和AtGLP2的调控模式与PnGLP相反，可能反映了短日照植物和长日照植物之间的差异。为了研究叶型GLP gsne调控与光周期相关的分子机制，我们决定使用荧光素酶基因作为报告基因来分析这些基因的启动子区域。由于这些 ptomoteis 之间没有保守的顺式元件，我们目前正在分析一系列启动子删除构建体，以确定哪个序列基序对于昼夜节律表达至关重要。确定 PnGLP 对光周期诱导开花的影响。我们正在研究表达 PnGLP cDNA 的 tiansgenic 植物。我们使用短日照烟草植物（Nicotiona tabacum cv. Maryland Mammoth）作为宿主植物。与对照植物相比，表达PnGLP cDNA的烟草植物很可能形成花穗。表达 PnGLP cDNA 的烟草植物可能作为光周期诱导短日照植物开花的温和刺激剂。我们的实验目前正在进行这样的假设。