A study on biocatalysts immobilized in nanospace

纳米空间固定化生物催化剂的研究

基本信息

  • 批准号:
    13650839
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

The objectives of the research are to prepare stable biocatalyst systems encapsulated in nanoporous, hydrophobic space and to apply them to green-chemical organic synthesis in organic media. As a nanoporous material, we chose highly-ordered mesoporous silica with a pore size of 6-9 nanometer in diameter, which was prepared using Si(OEt)_4, and triblockcopolymer. The internal surface of the mesoporous silica was then modified by silane coupling agents having amino, hydroxyl, and carboxylic functional groups. Lipase enzyme was immobilized in the chemically-modified mesoporous silicas, and applied to esterification between an equimolar amount of carboxylic acid and alcohol under ambient conditions. We compared biocatalysis between lipase encapsulated in the chemically modified mesoporous silicas and commercially available lipase supported on Celite. We found several advantages: 1) Our lipase system showed 20 times as high biocatalysis in esterification as the conventional lipase, 2) the volume of our immobilized lipase is much smaller than that of Celite-supported lipase, 3) the stability of the lipase in mesoporous silica is much superior, and 4) no leaching of lipase from the pores of chemically-modified mesoporous silica was observed.
本课题的研究目标是在纳米多孔疏水空间中制备稳定的生物催化剂体系,并将其应用于有机介质中的绿色化学有机合成。作为纳米多孔材料,我们选择了孔径为6-9纳米的高有序介孔二氧化硅,该介孔二氧化硅是由Si(OEt)_4和三嵌段共聚物制备的。然后用具有氨基、羟基和羧基官能团的硅烷偶联剂修饰介孔二氧化硅的内表面。将脂肪酶固定在化学修饰的介孔二氧化硅中,并将其应用于等摩尔量的羧酸与醇之间的酯化反应。我们比较了包封在化学修饰的介孔二氧化硅中的脂肪酶和在Celite上负载的市售脂肪酶的生物催化作用。我们发现了几个优点:1)我们的脂肪酶体系在酯化反应中的生物催化作用是传统脂肪酶的20倍;2)我们的固定化脂肪酶的体积比白石石负载的脂肪酶的体积小得多;3)脂肪酶在介孔二氧化硅中的稳定性要好得多;4)化学修饰的介孔二氧化硅的孔中没有观察到脂肪酶的浸出。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T.Itoh, Y.Nishimura, M.kashiwagi, M.Onaka: "Efficient Lipase-catalyzed Enantioselective Acylation in an Ionic Liquid Solvent System"Ionic Liquids as Green Solvents : Progress and Prospects, ACS Symposium Series. 856. 251-261 (2003)
T.Itoh、Y.Nishimura、M.kashiwagi、M.Onaka:“离子液体溶剂系统中的高效脂肪酶催化对映选择性酰化”离子液体作为绿色溶剂:进展与前景,ACS 研讨会系列。
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    0
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T.Itoh, Y.Nishimura, M.Kashiwagi, M.Onaka: "Efficient Lipase-catalyzed Enantioselective Acylation in an Ionic Liquid Solvent Syste"Ionic.Liquids as Green Solvents ; Progress and Prospects, ACS Symposium Series 856. 251-261 (2003)
T.Itoh、Y.Nishimura、M.Kashiwagi、M.Onaka:“离子液体溶剂系统中的高效脂肪酶催化对映选择性酰化”离子液体作为绿色溶剂;
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    0
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T.Itoh, N.Ouchi, Y.Nishimura, H.S.Hui, N.Katada, M.Niwa, M.Onaka: "Novel Supporting Materials of Lipase PS Suitable for Use in an Ionic Liquid Solvent System"Green Chemistry. 5. 494-496 (2003)
T.Itoh、N.Ouchi、Y.Nishimura、H.S.Hui、N.Katada、M.Niwa、M.Onaka:“适用于离子液体溶剂体系的新型脂肪酶 PS 支持材料”绿色化学。
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    0
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ONAKA Makoto其他文献

ONAKA Makoto的其他文献

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{{ truncateString('ONAKA Makoto', 18)}}的其他基金

Stabilization effects on labile chemical species by confinement in the zeolite's nanopores and its application to novel organic reactions
沸石纳米孔中的限制对不稳定化学物质的稳定作用及其在新型有机反应中的应用
  • 批准号:
    16H04562
  • 财政年份:
    2016
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of highly porous carbon nitraide and its application to catalytic reactions
高孔氮化碳的研制及其在催化反应中的应用
  • 批准号:
    25630361
  • 财政年份:
    2013
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
The Problem of Annexation of the Baltic States during the World WarII, 1939-1940.
第二次世界大战期间波罗的海国家的吞并问题,1939-1940。
  • 批准号:
    18730121
  • 财政年份:
    2006
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Development of Heterogeneous Metathesis Catalysts with Nanopores
纳米孔多相复分解催化剂的开发
  • 批准号:
    18065004
  • 财政年份:
    2006
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Development of Nanoporous Material-based Heterogeneous Catalysts for Metathesis
纳米孔材料基复分解多相催化剂的开发
  • 批准号:
    16360399
  • 财政年份:
    2004
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
THE DEVELOPMENT OF NANOPOROUS ALUMINA-SUPPORTED RHENIUM OXIDE CATALYST FOR OLEFIN METATHESIS
纳米孔氧化铝负载氧化铼烯烃复分解催化剂的研制
  • 批准号:
    11650805
  • 财政年份:
    1999
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
THE DEVELOPMENT OF NANO MEDIA FOR HIGHLY EFFICIENT SYNTHESIS OF PORPHYRINS
高效合成卟啉的纳米介质的开发
  • 批准号:
    10555277
  • 财政年份:
    1998
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
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