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A study on biocatalysts immobilized in nanospace

纳米空间固定化生物催化剂的研究

基本信息

批准号：
13650839
负责人：
ONAKA Makoto
金额：
$ 2.3万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13650839/
关键词：
Mesoporous silica Enzyme lipase Immobilized enzyme Biocatalyst system Green chemical process Sol-gel process Long-chain carboxylic acid Esterification グリーンケミストリー生体触媒メソ多孔体シリカ

项目摘要

The objectives of the research are to prepare stable biocatalyst systems encapsulated in nanoporous, hydrophobic space and to apply them to green-chemical organic synthesis in organic media. As a nanoporous material, we chose highly-ordered mesoporous silica with a pore size of 6-9 nanometer in diameter, which was prepared using Si(OEt)_4, and triblockcopolymer. The internal surface of the mesoporous silica was then modified by silane coupling agents having amino, hydroxyl, and carboxylic functional groups. Lipase enzyme was immobilized in the chemically-modified mesoporous silicas, and applied to esterification between an equimolar amount of carboxylic acid and alcohol under ambient conditions. We compared biocatalysis between lipase encapsulated in the chemically modified mesoporous silicas and commercially available lipase supported on Celite. We found several advantages: 1) Our lipase system showed 20 times as high biocatalysis in esterification as the conventional lipase, 2) the volume of our immobilized lipase is much smaller than that of Celite-supported lipase, 3) the stability of the lipase in mesoporous silica is much superior, and 4) no leaching of lipase from the pores of chemically-modified mesoporous silica was observed.

本课题的研究目标是在纳米多孔疏水空间中制备稳定的生物催化剂体系，并将其应用于有机介质中的绿色化学有机合成。作为纳米多孔材料，我们选择了孔径为6-9纳米的高有序介孔二氧化硅，该介孔二氧化硅是由Si(OEt)_4和三嵌段共聚物制备的。然后用具有氨基、羟基和羧基官能团的硅烷偶联剂修饰介孔二氧化硅的内表面。将脂肪酶固定在化学修饰的介孔二氧化硅中，并将其应用于等摩尔量的羧酸与醇之间的酯化反应。我们比较了包封在化学修饰的介孔二氧化硅中的脂肪酶和在Celite上负载的市售脂肪酶的生物催化作用。我们发现了几个优点：1)我们的脂肪酶体系在酯化反应中的生物催化作用是传统脂肪酶的20倍；2)我们的固定化脂肪酶的体积比白石石负载的脂肪酶的体积小得多；3)脂肪酶在介孔二氧化硅中的稳定性要好得多；4)化学修饰的介孔二氧化硅的孔中没有观察到脂肪酶的浸出。