Identification of juvenile hormone receptor by utilizing its inhibiting nature on 20E signaling

利用保幼激素受体对 20E 信号传导的抑制特性鉴定其

• 批准号: 13660046
• 负责人: MIURA Ken
• 金额: $ 2.3万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660046/
• 关键词: ecdysone; juvenile hormone; transcription Factor; 幼虫ホルモン; レセプター

The homologues of the coactivator, CREB-binding protein (CBP), the nuclear receptor, estrogen-related receptor (ERR), arylhydrocarbon receptor (AhR) and its heterodimeric partner, arylhydrocarbon receptor nuclear translocator (Arnt) were cloned from the mosquito Anopheles Stephensi. The mosquito CBP cDNA in the size of 10,974 bp encoded a pollypeptide with 3,658 amino acids. The mosquito ERR cDNA comprised 3,516bp, which encoded a polypeptide with 505 amino acids. The mosquito AhR was revealed to be composed of three alternativily spliced isoforms, namely AsAhR-a, -b, and -c. Both the a and c isoforms encoded polypeptides with over 980 amino acids, while the b isoform was a truncated version with 538 amino acids. As for the mosquito Arnt, it comprised two isoforms, which differed only in N-terminal vicinity. These mosquito homologues exhibited moderate amino acid sequence similarity against their vertebrate counterparts while showing the highest similarity to the Drosophila homologues.

从斯氏按蚊中克隆出了共激活物creb结合蛋白（CBP）、核受体、雌激素相关受体（ERR）、芳烃受体（AhR）及其异二聚体伙伴芳烃受体核转运子（Arnt）的同源物。蚊子CBP cDNA全长10,974 bp，编码一个包含3,658个氨基酸的多肽。蚊子ERR cDNA全长3516bp，编码了505个氨基酸的多肽。蚊子的AhR由三种选择性剪接的亚型组成，即AsAhR-a、-b和-c。a和c两种异构体编码的多肽都含有980多个氨基酸，而b异构体是一个截断的版本，含有538个氨基酸。蚊子的Arnt包含两个同工异构体，仅在n端附近不同。这些蚊子同源物与脊椎动物同源物具有中等程度的氨基酸序列相似性，而与果蝇同源物的相似性最高。

项目成果

Wojtased et al.: "Analysis of involvement of the 3'-untranslated regions in regulating mRNA stability for vitellogenin, cyanoprotein alpha, and cyanoprotein beta from the bean bug, Riptortus clavatus"Arch. Insect. Biochem. Physiol.. 51. 27-36 (2002)

Wojtased 等人：“分析豆蝽 Riptortus clavatus 中卵黄蛋白原、氰基蛋白 α 和氰基蛋白 β 的 3-非翻译区参与调节 mRNA 稳定性”Arch。

Miura et al.: "A Coup-TF/Svp homolog is highly expressed during vitellogenesis in the mosquito Aedes aegypti"J. Mol. Endocrinol.. 29. 223-238 (2002)

Miura 等人：“Coup-TF/Svp 同源物在埃及伊蚊的卵黄发生过程中高度表达”J.

Miura et al.: "A Coup-TF/Svp homolog is highly expressed during vitellogenesis in the mosquito Aedes aegypti"J.Mol.Endocrinol.. 29. 223-238 (2002)

Miura 等人：“Coup-TF/Svp 同源物在埃及伊蚊的卵黄发生过程中高度表达”J.Mol.Endocrinol.. 29. 223-238 (2002)