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Role of miRNAs on the regulation of juvenile hormone synthesis in mosquitoes

miRNA对蚊子保幼激素合成调节的作用

基本信息

批准号：
10176404
负责人：
Marcela Nouzova
金额：
$ 14.75万
依托单位：
FLORIDA INTERNATIONAL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10176404
关键词：
Adult Aedes Animals Area Basic Science Binding Biogenesis Biology Blood Chemicals Chikungunya fever Coenzyme A Corpora Allata Culicidae Dengue Dengue Fever Development Digestion Enzymes Event Excretory function Experimental Models Female Gene Expression Gene Expression Regulation Genes Genetic Transcription Goals High-Throughput Nucleotide Sequencing Hormonal Immunoprecipitation In Vitro Incidence Insect Control Insect Viruses Insecta Insecticides Intake Juvenile Hormones Knowledge Lead Libraries Link Liquid substance Malaria Mass Spectrum Analysis Messenger RNA MicroRNAs Molting Monitor Mosquito Control Mosquito-borne infectious disease Nutrient Nutritional status Organism Ovary Pathway interactions Physiological Plants Play Proteins Public Health Pupa Quantitative Reverse Transcriptase PCR Regulation Regulator Genes Reproduction Reproductive Process Research Resistance Role Sampling Signal Transduction Small RNA Testing Tissues Transcript Untranslated RNA ZIKA absorption base chikungunya design differential expression egg experience experimental study hormonal signals hormone biosynthesis in vivo inhibitor/antagonist novel strategies public health relevance success sugar transcriptome transcriptome sequencing transcriptomics vector control zika fever

项目摘要

PROJECT SUMMARY Recent dramatic increases in the incidence of mosquito-borne diseases, like Malaria, Zika, Chikungunya and Dengue Fever, and the wide-spread resistance of mosquitoes to insecticides emphasizes the need for new approaches for insect control based on mosquito-specific agents. The discovery of such mosquito-specific control agents depends on continued basic research on the biology of mosquitoes. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression in organisms as diverse as plants, animals, insects and viruses. Sequence specific binding of miRNAs to complementary regions on mRNAs can either down-regulate or up- regulate expression of the encoded protein. A growing body of evidence indicates that miRNAs contribute to the control of tissue- and stage-specific gene expression in mosquitoes; playing key roles modulating sugar absorption, fluid excretion, blood intake and digestion, egg maturation and survival in Ae. aegypti. On the other hand, little is known about the role of miRNAs on the regulation of juvenile hormone (JH) biosynthesis in insects. In the female mosquito, JH signals the completion of the ecdysis to the adult stage, and initiates reproductive processes. JH titer is essentially determined by the rate at which the corpora allata (CA) synthesizes JH. High- throughput small RNA sequencing of CA showed dramatic alterations of miRNA profiles among the CA of pupa (low JH synthesis), sugar-fed (high JH synthesis) and blood fed (low JH synthesis) adult females. We now intend to further study the role of CA-specific and differentially expressed miRNAs in the regulation of JH biogenesis. Completing the proposed research could lead to the identification of targets for designing new, specific and affordable strategies suitable for mosquito control.

项目总结

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Juvenile hormone mediates lipid storage in the oocytes of Dipetalogaster maxima.

DOI：
10.1016/j.ibmb.2020.103499
发表时间：
2020-11
期刊：
Insect biochemistry and molecular biology
影响因子：
3.8
作者：
F. O. Ramos;J. Leyria;M. Nouzová;Leonardo L. Fruttero;F. G. Noriega;L. Canavoso
通讯作者：
F. O. Ramos;J. Leyria;M. Nouzová;Leonardo L. Fruttero;F. G. Noriega;L. Canavoso

Approaches and Tools to Study the Roles of Juvenile Hormones in Controlling Insect Biology.

DOI：
10.3390/insects11120858
发表时间：
2020-12-03
期刊：
Insects
影响因子：
3
作者：
Noriega FG;Nouzova M
通讯作者：
Nouzova M

Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.

DOI：
10.1038/s41598-022-25009-4
发表时间：
2022-11-28
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Nouzova, Marcela;Edwards, Marten J.;DeGennaro, Matthew;Leyva, Dennys;Tose, Lilian V.;Fernandez-Lima, Francisco;Noriega, Fernando G.
通讯作者：
Noriega, Fernando G.

Role of Methoprene-tolerant in the regulation of oogenesis in Dipetalogaster maxima.

DOI：
10.1038/s41598-022-18384-5
发表时间：
2022-08-20
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Ramos, Fabian O.;Nouzova, Marcela;Fruttero, Leonardo L.;Leyria, Jimena;Ligabue-Braun, Rodrigo;Noriega, Fernando G.;Canavoso, Lilian E.
通讯作者：
Canavoso, Lilian E.

Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage.