Biodegradation of photodegradable coating materials used for coated fertilizers by soil microorganisms

土壤微生物对包膜肥料用光降解包膜材料的生物降解

• 批准号: 13660069
• 负责人: SAKAI Masao
• 金额: $ 2.11万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660069/
• 关键词: Photodegradable polyethylenes; long-chain n-alkanes; rDNA; BOD; Coated fertilizers; 長鎖n-アルカン; 糸状菌; PCR-DGGE; 強酸性茶園土壌; コーティング肥料; 長鎖n-アルカン資化細菌

Photodegradable polyethylenes, which produce alkane-like substances following sustained exposure to sunlight, are used as a coating material for some agricultural fertilizers. This study examines the biodegradability of these substances in two different agricultural soil types using the biochemical oxygen demand (BOD) method After a 21 day test period, high molecular weight photodegradation products showed little biodegradation. However, the low molecular weight fractions extracted from the photodegradation products using hexane showed biodegradabilities of 8% to 18% within the same 21 day period. Furthermore, the two soils were found to have different biodegradation patterns, possibly due to differences in the microbial communities present. These results suggest that microbes that can degrade and utilize the low molecular weight photodegradation products of photodegradable polyethylenes exist in the tested agricultural soils.Microorganisms utilizing alkanes are widely distributed in nature, and such microorganisms are isolated from various locations. However, microorganisms that degrade and utilize long-chain n-alkanes have not been reported from acidic soil. In this study, we examined highly acidic soil samples (pH 3.2) collected from a tea field, and attempted to isolate and identify microorganisms utilizing long-chain n-alkanes. No bacteria utilizing long-chain n-alkanes were found, but filamentous fungi with the ability of utilizing long-chain n-alkanes were obtained. Phylogenetic analysis using SSU rDNA sequences suggested that the fungi obtained were Cordyceps sp. and Mortierella sp.

可光降解的聚乙烯在持续暴露于阳光下后产生类似烷烃的物质，被用作某些农业肥料的涂层材料。本研究探讨了这些物质在两种不同的农业土壤类型的生化需氧量（BOD）的方法，经过21天的测试期后，高分子量的光降解产物表现出很小的生物降解性。然而，使用己烷从光降解产物中提取的低分子量组分在相同的21天内显示出8%至18%的生物降解性。此外，这两种土壤被发现有不同的生物降解模式，可能是由于存在的微生物群落的差异。这些结果表明，在供试的农业土壤中存在能够降解和利用光降解聚乙烯的低分子量光降解产物的微生物，利用烷烃的微生物在自然界中广泛分布，并从不同的地方分离到这类微生物。然而，在酸性土壤中降解和利用长链正构烷烃的微生物还未见报道。在这项研究中，我们研究了高酸性土壤样品（pH 3.2），从茶园收集，并试图分离和鉴定微生物利用长链正构烷烃。没有发现利用长链正构烷烃的细菌，但获得了具有利用长链正构烷烃能力的丝状真菌。利用SSU rDNA序列进行系统发育分析表明，所分离的真菌分别为虫草属（Cordyceps sp.）和被孢霉属（Mortierella sp.）

项目成果

Sakai, M. et al.: "Biodegradability of photodegradable polyethylenes used for coating of coated fertilizers in agricultural soils"Japanese J. Soil Sci. Plant Nutr.. 75. 79-81 (2004)

Sakai，M.等人：“用于农业土壤中包膜肥料包膜的可光降解聚乙烯的生物降解性”日本土壤科学杂志。

Sakai, M. et al.: "Biodegradability of photodegradable coating materials used for coated fertilizers in agricultural soils"Soil Sci. Plant Nutr.. 49. 647-650 (2003)

Sakai，M.等人：“用于农业土壤中包膜肥料的光降解包膜材料的生物降解性”土壤科学。

Sakai, M.et al.: "Biodegradability of photodegradable coating materials used for coated fertilizers in agricultural soils"Soil Sci.Plant Nutr.. 49. 647-650 (2003)

Sakai，M.等：“农业土壤中用于包膜肥料的光降解包膜材料的生物降解性”Soil Sci.Plant Nutr.. 49. 647-650 (2003)

境 雅夫ら: "被覆肥料の被覆材として使用される光分解性ポリエチレンの畑土壌における生分解性"日本土壌肥料学雑誌. 75. 79-81 (2004)

Masao Sakai 等人：“用作田间土壤肥料覆盖材料的可光降解聚乙烯的生物降解性”日本土壤与肥料杂志 75. 79-81 (2004)。