EVOLUTION OF BACTERIAL AROMATIC-DEGRADING OPERONS BY ALTERATION OF THE MECHANISM OF TRANSCRIPTIONAL REGULATION

通过改变转录调控机制来进化细菌芳香降解操纵子

• 批准号: 13660080
• 负责人: NOJIRI Hideaki
• 金额: $ 2.56万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660080/
• 关键词: Transcriptional regulation; Operon; car gene cluster; Pseudomonas; Biodegradation of Aromatics; Evolution; Dioxin; Carbazole

Anthranilate (AN)-dependant P_<ant> promoter regulates the transcription of ant and car operons of P. resinovorans strain CA10, which encode the enzymes involved in CAR-degradation to AN and AN conversion to catechol, respectively. Genetic region involved in the transcriptional activation, a regulator protein, an effector molecule, and transcriptional start points of P_<ant> promoter have been characterized. δ^s・dependant near-constitutive P_<carAa> promoter is also involved in the transcription of car operon. P_<ant> promoter is originally located in the upstream region of ant operon, and the transposition of ISPre1 creates the additional copy of P_<ant> at the upstream of car operon. Resultantly, car operon became to be also induced strongly by AN, which is a metabolic intermediate of CAR.New CAR-degrading bacteria have been isolated from environmental samples. Genetic analyses of CAR-degrading car operons revealed that car operon of Janthinohacterium sp. strain J3 is an ancestral-type of that of P. resinovorans strain CA10. car operon of strain J3 is regulated by P_<u13> promoter, which is regulated by transcriptional repressor CarR. The effecter molecule of CarR is an unstable meta-cleavage compound in CAR degradative pathway. Trascriptional regulation mechanism and the expression profile in car operons of strain J3 are clearly different from that of strain CA10. It is shown that the recruitment of P_<ant> promoter by ISPre1 transposition has resulted in the effective CAR-degrading ability.

邻氨基苯甲酸盐（AN）依赖的P_<ant>启动子调控噬脂假单胞菌CA_（10）的ant和car操纵子的转录，这两个操纵子分别编码CAR降解为AN和AN转化为邻苯二酚的酶。研究了P_启动子转录激活的基因区、调控蛋白、效应分子和转录起始点<ant>。依赖δ^s·的近组成型P_<carAa>启动子也参与car操纵子的转录。P_<ant>promoter最初位于ant操纵子的上游区域，ISPre1的转座在car操纵子的上游产生了P_promoter的额外拷贝<ant>。因此，CAR操纵子也被CAR的代谢中间产物AN强烈诱导。CAR降解car操纵子的遗传分析显示，Janthinohacterium sp.菌株J3的car操纵子是P.resinovorans菌株CA 10的祖先型。菌株J3的car操纵子受P_<u13>启动子调控，而P_启动子受转录抑制子卡尔R调控。卡尔受体的效应分子是CAR降解途径中不稳定的间位裂解化合物。J3株的转录调控机制和汽车操纵子的表达谱与CA 10株明显不同。结果表明，<ant>通过ISPre1转座募集P_启动子导致了有效的CAR降解能力。

项目成果

Kengo Inoue 他11名: "Divergent structures of carbazole degradative car operons isolated from Gram-negative bacteria"Bioscience, Biotechnology, and Biochemistry. In press. (2004)

Kengo Inoue 和其他 11 人：“从革兰氏阴性细菌中分离出的咔唑降解汽车操纵子的不同结构”《生物科学、生物技术和生物化学》已出版（2004 年）。

Divergent structures of carbazole degradative car operons isolated from Gram-negative bacteria.

从革兰氏阴性细菌中分离出的咔唑降解汽车操纵子的不同结构。

• 发表时间: 2004
• 期刊: Bioscience, Biotechnology, and Biochemistry 68
• 作者: Inoue et al.

Divergent structures of the carbazole degradative car operons isolated from Gram-negative bacteria.

从革兰氏阴性细菌中分离出的咔唑降解汽车操纵子的不同结构。

• 发表时间: 2004
• 期刊: Bioscience, Biotechnology, and Biochemistry 68
• 作者: Inoue;K.;Widada;J.;Nakai;S.;Endoh;T.;Urata;M.;Ashikawa;Y.;Shintani;M.;Saiki;Y.;Yoshida;T.;Habe;H.;Omori;T.;Nojiri;H.
• 通讯作者: H.

Transcriptional regulation of the ant operon, encoding two-component anthranilate 1,2-dioxygenase, on the carbazole-degradative plasmid pCAR1 of Pseudomonas resinovorans strain CA10

• DOI: 10.1128/jb.186.20.6815-6823.2004
• 发表时间: 2004-10-01
• 期刊: JOURNAL OF BACTERIOLOGY
• 影响因子: 3.2
• 作者: Urata, M;Miyakoshi, M;Nojiri, H
• 通讯作者: Nojiri, H