Molecular bases of electron transport component selectivity in aromatic ring-hydroxylating dioxygenase system

芳环羟基化双加氧酶系统电子传递组分选择性的分子基础

• 批准号: 20248010
• 负责人: NOJIRI Hideaki
• 金额: $ 31.2万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20248010/
• 关键词: 芳香族化合物; オキシゲナーゼ; 電子伝達; タンパク質間相互作用; 結晶構造; 結合定数; biodegradation; 細菌; 芳香環水酸化オキシゲナーゼ; タンパク質問相互作用

Crystal structures of reduced form of ferredoxin component (Fd) and complex form of oxidized Fd and reduced oxygenase (Oxy) of carbazole 1,9a-dioxygeanse were solved. Cleavages of salt-bridge between Oxy R118 and Fd E43 and hydrogen-bonding between Oxy R210 and Fd E55 were detected along with the reduction of Oxy. Alanine replacement analyses revealed these bindings are cooperatively enhanced the binding between Oxy and Fd. Isothermal titration calorimetry analyses also revealed that the binding through hydrophobic interaction between the components were stabilized by surrounding salt-bridge and hydrogen-bonding.

解析了铁氧还蛋白组分（Fd）的还原态和咔唑1，9 α-二氧合酶的氧化态Fd与还原态加氧酶（Oxy）的复合态的晶体结构。Oxy R118和Fd E43之间的盐桥断裂以及Oxy R210和Fd E55之间的氢键沿着Oxy的还原。丙氨酸替代分析显示这些结合协同增强Oxy和Fd之间的结合。等温滴定量热分析还表明，通过疏水相互作用的结合的组分之间的稳定周围的盐桥和氢键。

项目成果

含窒素芳香族化合物初発酸化酵素carbazole 1,9a-dioxygenaseにおける酸化酵素-ferredoxin間の結合解離メカニズムの解明

阐明含氮芳香族化合物的初级氧化酶咔唑1,9a-双加氧酶中氧化酶与铁氧还蛋白之间的键解离机制

• 发表时间: 2012
• 作者: 松澤、野尻;ら7名
• 通讯作者: ら7名

クラスIII型carbazole 1,9a-dioxygenaseにおけるferredoxin-terminal oxygenase間の結合乖離メカニズムの解明

阐明 III 类咔唑 1,9a-双加氧酶中铁氧还蛋白和末端加氧酶之间的解离机制

• 发表时间: 2012
• 作者: 松澤、野尻;ら6名
• 通讯作者: ら6名

Crystallization and preliminary X-ray diffraction studies of a ferredoxin reductase component of carbazole 1,9a-dioxygenase from Novosphingobium sp.KA1

来自Novosphingobium sp.KA1的咔唑1,9a-双加氧酶的铁氧还蛋白还原酶组分的结晶和初步X射线衍射研究

• 发表时间: 2010
• 期刊: Acda Crystallographica Section F.Structural Biology and Crystallization Communication
• 作者: Umeda T;Katsuki J;Ashikawa Y;Usami Y;Inoue K;Noguchi H;Fujimoto Z;Yamane H;Nojiri H
• 通讯作者: Nojiri H

Crystallization and preliminary X-ray diffraction studies of a terminal oxygenase of carbazole 1,9a-dioxygenase from Novosphingobium sp.KA1

新鞘氨醇菌KA1咔唑1,9a-双加氧酶末端加氧酶的结晶和初步X射线衍射研究

• 发表时间: 2010
• 期刊: Acda Crystallographica Section F.Structural Biology and Crystallization Communication
• 作者: Umeda T;Katsuki J;Ashikawa Y;Usami;Y;Inoue K;Noguchi H;Fujimoto Z;Yamane H;Nojiri H
• 通讯作者: Nojiri H

Specific Interactions between the Ferredoxin and Terminal Oxygenase Components of a Class IIB Rieske Nonheme Iron Oxygenase, Carbazole 1,9a-Dioxygenase

• DOI: 10.1016/j.jmb.2009.07.029
• 发表时间: 2009-09-18
• 期刊: JOURNAL OF MOLECULAR BIOLOGY
• 影响因子: 5.6
• 作者: Inoue, Kengo;Ashikawa, Yuji;Nojiri, Hideaki
• 通讯作者: Nojiri, Hideaki