Regulation of Rubisco operon in red algal lineage

红藻谱系中 Rubisco 操纵子的调控

• 批准号: 24870005
• 负责人: MINODA Ayumi
• 金额: $ 1.83万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24870005/
• 关键词: ルビスコ; 紅藻; YCf30; 葉緑体; 転写制御; Ycf30; 光合成; CO2; 光; 進化; 転写因子

During evolution, ancestral plastids arose from a single endosymbiosis and have lost their autonomy through integration into the eukaryotic cell. However, a transcription factor, Ycf30 is retained in currently known plastid genomes of red algal and Glaucophyte lineages. Previously, we suggested transcriptional activation model of Rubisco operon by Ycf30 through sensing of photosynthetic metabolites in response to the environmental changes.In this report, to test the model in vivo, we analyzed metabolites in response to the environmental changes. During the shift from high CO2 condition to air condition, 3-PGA and RuBP were specially accumulated. This result is agreed with the model suggested previously. Ycf30 senses the change in the accumulations of photosynthetic metabolites and activates the transcription of Rubisco operon in response to the environmental changes. Red algal plastidial transcription factors represent the last relics of the autonomy of the ancestral plastid.

在进化过程中，祖先质体从单一的内共生中产生，并通过整合到真核细胞中而失去了自主性。然而，转录因子Ycf 30保留在目前已知的红藻和绿藻谱系的质体基因组中。以前，我们提出了Rubisco操纵子通过Ycf 30感知光合代谢产物响应环境变化而转录激活的模型，在本报告中，我们在活体上分析了响应环境变化的代谢产物，以验证该模型。在由高CO_2条件向空气条件转换的过程中，3-PGA和RuBP在细胞内发生了特异性积累。这一结果与以前提出的模型一致。Ycf 30感知光合代谢产物积累的变化，并激活Rubisco操纵子的转录以响应环境变化。红藻质体转录因子代表了祖先质体自主性的最后遗迹。

项目成果

硫酸性温泉紅藻Galdieria sulphurariaによるランタノイドの回収

硫磺温泉红藻 Galdieria sulfuraria 回收镧系元素

• 发表时间: 2012
• 作者: 蓑田歩;山本高郁;鈴木石根;都筑幹夫
• 通讯作者: 都筑幹夫

循環型エネルギーを利用した硫酸性温泉紅藻Galdieria sulphurariaによるレアメタル回収システム

利用硫酸温泉红藻Galdieria sulfuraria利用循环能源的稀有金属回收系统

• 发表时间: 2012
• 作者: 蓑田歩;山本高郁;都筑幹夫
• 通讯作者: 都筑幹夫

External light conditions and internal cell cycle phases coordinate accumulation of chloroplast and mitochondrial transcripts in the red alga Cyanidioschyzon merolae.

• DOI: 10.1093/dnares/dss013
• 发表时间: 2012-06
• 期刊: DNA research : an international journal for rapid publication of reports on genes and genomes
• 作者: Kanesaki Y;Imamura S;Minoda A;Tanaka K
• 通讯作者: Tanaka K