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Cell-cell communication in the ovarian tissue and its regulation by pituitary prolactin : in case of GnRH and annexin 5

卵巢组织中的细胞间通讯及其垂体催乳素的调节：以 GnRH 和膜联蛋白 5 为例

基本信息

批准号：
13660306
负责人：
HASHIMOTO Inoru
金额：
$ 2.05万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660306/
关键词：
pseudopergnancy GnRH corpus luteum prolactin annexin 5 Cell-cell communication progesterone apoptosis 卵巣黄体形成

项目摘要

In the ovarian tissue, the expression of annexin 5, a calcium-phospholipid binding protein and a novel target of GnRH, in the corpus luteum is suppressed by prolactin. During pseudopregnancy of rats in which two daily prolactin surges occur, inhibition of prolactin secretion by dopamine agonist, 2α-bromoergocriptine mesylate (CB-154), resulted in the augmentation of annexin 5 expression and the occurrence of TUNEL positive reaction in the same luteal cells. Local administration of GnRH antagonist, Cetrorelix, to unilateral ovarian bursa of a CB-154 administered pseudopregnant rat with an osmotic pump completely suppressed both apoptosis and annexin 5 expression, while they were observed in the luteal cells of the contralateral non-treated ovary. The direct effect of GnRH on luteal cells was examined by administering GnRH agonist (GnRHa) with an osmotic pump to hemilateral ovarian bursa of a pseudopregnant rat. GnRHa induced apoptosis and annexin 5 expression in the same luteal cells. Since the administration of progesterone did not suppressed apoptosis and annexin 5 expression in the CB-154 treated pseudopregnant rats, prolactin is thought to inhibit GnRH action directly in the ovary. Getting together, it is suggested that prolactin maintains the functional luteal phase at least partly by suppressing local GnRH action. (this work was published in Endocrinology 2003 144:3625-3631).

在卵巢组织中，膜联蛋白5（一种钙磷脂结合蛋白和GnRH的新靶点）在黄体中的表达受到催乳素的抑制。在每天出现两次催乳素高峰的大鼠假绝经期间，多巴胺激动剂2α-溴麦角隐亭甲磺酸盐（CB-154）抑制催乳素分泌，导致同一黄体细胞中Annexin 5表达增加和TUNEL阳性反应的发生。GnRH拮抗剂，西曲瑞克，局部给药的CB-154管理的假孕大鼠的单侧卵巢囊与渗透泵完全抑制细胞凋亡和膜联蛋白5的表达，而它们被观察到在对侧未处理的卵巢的黄体细胞。用渗透泵将促性腺激素释放激素激动剂（GnRHa）注入假孕大鼠一侧卵巢囊，观察促性腺激素释放激素（GnRH）对黄体细胞的直接作用。GnRHa诱导同一黄体细胞凋亡和膜联蛋白5的表达。由于孕激素给药不能抑制CB-154处理的假孕大鼠的细胞凋亡和膜联蛋白5表达，因此认为催乳素直接抑制卵巢中的GnRH作用。总之，催乳素至少部分通过抑制局部GnRH作用维持功能性黄体期。(this工作发表于Endocrinology 2003 144：3625-3631）。