Function of Dell in Embryonic Development

戴尔在胚胎发育中的作用

• 批准号: 13670027
• 负责人: HIDAI Chiaki
• 金额: $ 1.98万
• 依托单位: Nihon University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670027/
• 关键词: Endothelia cell; Development; Angiogenesis; 形態形成; 心筋; 肥大

Vascular remodeling is an essential process that allows the primary vascular plexus to develop into matured vasculature during embryonic development. Gene-targeting experiments have revealed that such remodeling is regulated by the products of several different genes. These include secreted molecules, receptor tyrosine kinases, and transcription factors. In mutant mice lacking these molecules, the primary vascular plexus fails to assemble into large vessels during development. Unfortunately, these 'loss of function' experiments do not advance our understanding of how vascular remodeling controls branching morphogenesis during development. Dell is an extracellular matrix protein that is exclusively expressed in embryonic endothelial cells. To investigate Dell function in developing embryos, we used a cytomegalovirus enhancer seguence to generate Dell overexpression mice, using. In transgenic mice, the total vascular bed was decreased in the alimentary tracts because of accelerated vascular remodeling at mid stage gestation. In addition to the quantitative change, overexpression of Dell reorganized vascular branching patterns, resulting in dendritic vessels. The present study indicates that Dell overexpression accelerates vascular remodeling and causes changes in branching morphogenesis during embryonic development

血管重塑是胚胎发育过程中初级血管丛发育为成熟血管的重要过程。基因靶向实验表明，这种重塑是由几个不同基因的产物调节的。这些包括分泌分子、受体酪氨酸激酶和转录因子。在缺乏这些分子的突变小鼠中，初级血管丛在发育过程中无法组装成大血管。不幸的是，这些“功能丧失”的实验并没有推进我们对血管重塑如何控制发育过程中分支形态发生的理解。Dell是一种细胞外基质蛋白，仅在胚胎内皮细胞中表达。为了研究Dell在胚胎发育中的功能，我们使用巨细胞病毒增强子序列产生Dell过表达小鼠，使用。在转基因小鼠中，总血管床减少，在消化道，因为在中期妊娠加速血管重塑。除了数量上的变化，过表达戴尔重组血管分支模式，导致树突状血管。目前的研究表明，戴尔过度表达加速血管重塑，并导致在胚胎发育过程中的分支形态发生的变化

项目成果

Aoka Y, Johnson FL, Penta K, Hirata Ki K, Hidai C, Schatzman R, Vamer JA, Quertermous T.: "The embryonic angiogenic factor dell accelerates tumor growth by enhancing vascular formation"Microvasc Res.. 64. 148-161 (2002)

Aoka Y、Johnson FL、Penta K、Hirata Ki K、Hidai C、Schatzman R、Vamer JA、Quertermous T.：“胚胎血管生成因子 dell 通过增强血管形成来加速肿瘤生长”Microvasc Res.. 64. 148-161（

Aoka Y: "The embryonic angiogenic factor Del1 accelerates tumor growth by enhancing vascular formation"Microvascular Research. 64(1). 148-161 (2002)

Aoka Y：“胚胎血管生成因子 Del1 通过增强血管形成加速肿瘤生长”微血管研究。

日台 智明 他7名: "The embryonic angiogenic factor dell accelerates tumor growth by enhancing vascular formation"Microvascular Res.. 64. 148-161 (2002)

Tomoaki Nichidai 等 7 人：“胚胎血管生成因子 dell 通过增强血管形成来加速肿瘤生长”微血管研究 64. 148-161 (2002)