EFFECTS OF ACTIVATING THE CHOLINERGIC VASODILATIVE SYSTEM ORIGINATING IN THE BASAL FOREBRAIN ON BLOOD FLOW AND DELAYED NEURONAL DEATH FOLLOWING TRANSIENT ISCHEMIA

激活源自基底前脑的胆碱能血管舒张系统对短暂性缺血后血流和延迟性神经元死亡的影响

• 批准号: 13670078
• 负责人: HOTTA Harumi
• 金额: $ 1.79万
• 依托单位: Tokyo Metropolitan Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670078/
• 关键词: BASAL FOREBRAIN; CEREBRAL CORTEX; HIPPOCAMPUS; RAT; REGIONAL CEREBRAL BLOOD FLOW; NUCLEUS BASALIS OF MEYNERT; DELAYED NEURONAL DEATH; TRANSIENT CEREBRAL ISCHEMIA; 中隔野; 電気刺激; 一過性虚血

An increase in cortical cerebral blood flow (CBF), independent of metabolic vasodilation, via the activation of cholinergic neurons originating in the nucleus basalis of Meynert (NBM) and septal complex in the basal forebrain and projecting to the cortex and hippocampus was recently demonstrated. In the present study, we aimed to clarify whether the increase in CBF following stimulation of the NBM or septal complex can improve delayed death of the cortical or hippocampal neurons following transient ischemia in rats.Cortical and hippocampal CBF was measured using a laser Doppler flowmeter, and delayed neuronal death of the cerebral cortex or hippocampus produced by intermittent (every 5 s) occlusions of the unilateral common carotid artery for 60 min was measured histologically in the cortical hemisphere. In the case of study on hippocampus, permanent ligation of bilateral vertebral arteries was previously performed.The increase in cortical CBF induced by NBM stimulation prevented the occlusion-induced decrease in cortical CBF, and the delayed death of the cortical neurons previously observed after the occlusions was scarcely observable. The increase in hippocampal CBF induced by stimulating the medial septum prevented the occlusion-induced decrease in hippocampal CBF, and the delayed death of the hippocampal CA1 neurons previously observed after the occlusions was scarcely observable.The present results suggest that basal forebrain-originating vasodilative activation can protect ischemia-induced delayed death of cortical and hippocampal neurons by preventing a blood flow decrease in cortex and hippocampus.

最近证实，皮质脑血流量（CBF）的增加，独立于代谢性血管舒张，通过激活胆碱能神经元起源于Meynert基底核（NBM）和隔复合体在基底前脑和投射到皮质和海马。在本研究中，我们的目的是澄清是否增加CBF后刺激的NBM或隔复合体可以改善延迟死亡的皮质或海马神经元短暂缺血大鼠。皮质和海马CBF测量使用激光多普勒流量计，和大脑皮层或海马的迟发性神经元死亡（每5秒）单侧颈总动脉闭塞60分钟在皮质半球中进行组织学测量。在海马的研究中，先前进行了双侧椎动脉的永久性结扎，NBM刺激引起的皮质CBF增加阻止了闭塞引起的皮质CBF减少，并且几乎没有观察到先前观察到的闭塞后皮质神经元的延迟死亡。刺激内侧隔引起的海马CBF的增加阻止了闭塞引起的海马CBF的减少，并且先前观察到的海马CA1区神经元的延迟死亡在闭塞后几乎没有观察到。本研究结果表明，基底前脑起源的血管舒张激活可以通过防止皮质和海马的血流量减少来保护缺血引起的皮质和海马神经元的延迟死亡。

项目成果

堀田晴美: "高齢者の体臭について"繊維機械学会誌. 56. 12-19 (2003)

Harumi Hotta：“关于老年人的体臭”《纤维机械学会杂志》56. 12-19（2003）。

Hotta, H., et al.: "Effects of stimulating the nucleus basalis of Meynert on blood flow and delayed neuonal death following transient ischemia in the rat cerebral cortex."Japanese Journal of Physiology. 52. 383-393 (2002)

Hotta, H. 等人：“刺激 Meynert 基底核对大鼠大脑皮层短暂性缺血后血流和延迟神经元死亡的影响。”日本生理学杂志。

Hotta, H., Uchida, S., Suzuki, A., Kagitani, F.: "Increases in regional blood flow in the cerebral cortex following activation of the nucleus basalis of Meynert by electrical stimulation can protect against ischemia-induced injury of cortical neurons in r

Hotta, H.、Uchida, S.、Suzuki, A.、Kagitani, F.：“通过电刺激激活 Meynert 基底核后大脑皮层局部血流量增加，可以防止缺血引起的皮质损伤

Suzuki, A., et al.: "The effects of age on somatocardiac reflexes in anesthetized rats."Japanese Journal of Physiology. 54. 137-141 (2004)

Suzuki, A., et al.：“年龄对麻醉大鼠躯体心脏反射的影响。”日本生理学杂志。

Hotta, H: "Stimulation of the nucleus basalis of Meynert increases diameter of the parenchymal blood vessels in rat's cerebral cortex."Neuroscience Letters. 358. 103-106 (2004)

Hotta，H：“Meynert 基底核的刺激会增加大鼠大脑皮层实质血管的直径。”《神经科学快报》。