Function of tyrosine phosphorylation of BIT in photic entrainment of the circadian clock in rats

BIT酪氨酸磷酸化在大鼠生物钟光牵引中的作用

• 批准号: 13670122
• 负责人: OKUMURA Nobuaki
• 金额: $ 1.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670122/
• 关键词: Circadian rhythm; BIT; SHIPS-1; Hypothalamus; Tyrosine phosphorylation; サーカディアンリズム; 光同調

Circadian rhythms of mammals are generated by a circadian oscillation of master pacemaker genes in the suprachiasmatic nucleus of the hypothalamus (SCN), and entrained by environmental factors such as 24-h light-dark cycles. We have previously shown that light exposure during the dark period enhanced tyrosine phosphorylation of brain immunoglobulin-like molecule with tyrosine-based activation motifs (BIT) in the rat SCN. Our major findings from last year were as follows, (1) BIT was tyrosine phosphorylated in response to light in the retina, (2) BIT was tyrosine phosphorylated on cold exposure in various nuclei of the hypothalamus, (3) BIT was associated with pl37GPI-anchored protein and clathrin heavy chain, (4) BIT was internalized by endocytosis and this process might be regulated by its tyrosine phosphorylation.

哺乳动物的昼夜节律是通过下丘脑（SCN）的肌核核中主是起搏器基因的昼夜节律振荡产生的，并由环境因素（例如24小时的浅黑色循环）所包含。我们先前已经表明，黑暗时期的光暴露增强了大鼠SCN中基于酪氨酸的活化基序（BIT）的脑免疫球蛋白样分子的酪氨酸磷酸化。我们去年的主要发现如下，（1）（1）位对视网膜中的光响应酪氨酸磷酸化。磷酸化。

项目成果

奥村宣明, 永井克也: "二次元ゲル電気泳動"ゲノミクス・プロテオミクスの新展開(エヌ・ティー・エス社). 468-473 (2004)

Nobuaki Okumura、Katsuya Nagai：“二维凝胶电泳”基因组学和蛋白质组学的新进展（NTS Inc.）468-473（2004）。

Yasushi Isojima, Nobuaki Okumura, Katsuya Nagai: "Molecular mechanism of mammalian circadian clock."J.Biochem.. 134. 777-784 (2003)

Yasushi Isojima、Nobuaki Okumura、Katsuya Nagai：“哺乳动物生物钟的分子机制。”J.Biochem.. 134. 777-784 (2003)

奥村宣明, 永井克也: "プロテオミクスにおける二次元電気泳動"Molecular Medicine. 39巻. 158-165 (2002)

Nobuaki Okumura、Katsuya Nagai：“蛋白质组学中的二维电泳”《分子医学》39. 158-165 (2002)。

Y.Nakahara et al.: "Light-induced Tyrosine Phosphorylation of BIT in the Rat Suprachiasmatic Nucleus"Journal of Neurochemistry. 74-11. 2436-2444 (2000)

Y.Nakahara 等人：“大鼠视交叉上核中 BIT 的光诱导酪氨酸磷酸化”神经化学杂志。

Hiroyuki Taniguchi, Nobuaki Okumura, Juri Hamada, Miho Inagaki, Yauskazu Nakahata, Shin-ichiro Sano, Katsuya Nagai: "Cold exposure induces tyrosine phosphorylation of BIT through NMDA receptors in the rat hypothalamus"Biochem.Biopohys.Res.Comm.. (in press

Hiroyuki Taniguchi、Nobuaki Okumura、Juri Hamada、Miho Inagaki、Yauskazu Nakahata、Shin-ichiro Sano、Katsuya Nagai：“冷暴露通过大鼠下丘脑中的 NMDA 受体诱导 BIT 酪氨酸磷酸化”Biochem.Biopohys.Res.Comm..（in