Genome-wide analyses of genes predominantly expressed in the mouse neocortex

主要在小鼠新皮质中表达的基因的全基因组分析

• 批准号: 13670142
• 负责人: TAKUMI Toru
• 金额: $ 2.3万
• 依托单位: Osaka Bioscience Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670142/
• 关键词: Cerebral neocortex; DNAchip; in situ hybridization; pyramidal neuron; PCP

The mammalian cerebral neocortex occupies the largest area of the cerebral cortex and is cytoarchitectually composed of six layers (I-VI). Many of neurological diseases including the mental illnesses are due to the dysfunction of the neocortex. In spite of its functional importance, the neocortex is virtually an unexplored region by approaches of molecular biology including the genome-wide technology. The global screening using microarrays, combined with the systematic in situ hybridization, has provided us with several candidate genes that are expressed predominantly in the mouse neocortex. Among them, Fez, a zinc finger type transcription factor, was analyzed in further detail. No other genes in the neocortex have been known so far to have their expression the strongest in terms of specificity. Using two color in situ hybridization, we have shown that Fez is mainly expressed in the cortical layer V, not in the GABA neurons but in the pyramidal neurons, the projection neurons of the cerebral cortex. Molecules identified by our systematic analyses would be invaluable not only for molecular understanding of the development and function of the neocortex but also for the use of their promoters in the gene-manipulated animals as well as in conditional expression systems.

哺乳动物大脑新皮质占据大脑皮质的最大面积，并且在细胞结构上由六层（I-VI）组成。包括精神疾病在内的许多神经系统疾病都是由于新皮层功能障碍造成的。尽管其功能的重要性，新皮层实际上是一个未开发的区域的分子生物学方法，包括全基因组技术。使用微阵列的全球筛选，结合系统的原位杂交，为我们提供了几个主要在小鼠新皮层表达的候选基因。其中，Fez，锌指型转录因子，进行了进一步的详细分析。到目前为止，在新皮层中还没有其他已知的基因在特异性方面具有最强的表达。利用双色原位杂交技术，我们发现Fez主要表达于皮层V层，而不是GABA神经元，而是锥体神经元，即大脑皮层的投射神经元。通过我们的系统分析确定的分子不仅对于新皮层的发育和功能的分子理解是非常宝贵的，而且对于在基因操纵的动物中以及在条件表达系统中使用它们的启动子也是非常宝贵的。

项目成果

T.Takumi: "A rapid cDNA cloning by PCR screening(RC-PCR)"Methods Mol Biol. 192. 385-389 (2002)

T.Takumi：“通过 PCR 筛选快速 cDNA 克隆（RC-PCR）”Methods Mol Biol。

H.Ohyama, H.Kajita, K.Omori, T.Takumi, N.Hiramoto, T.Iwasaka, H.Matsuda: "Inhibition of cardiac delayed rectifier K^+ currents by an antisense oligodeoxynucleotide against Isk (MinK) and over-expression of Isk mutant D77N in neonatal mouse hearts"Pflugers

H.Ohyama、H.Kajita、K.Omori、T.Takumi、N.Hiramoto、T.Iwasaka、H.Matsuda：“针对 Isk (MinK) 和过表达的反义寡脱氧核苷酸对心脏延迟整流 K^ 电流的抑制

S.Bouret, V.Prevot, T.Takumi, JC.Beauvillain, V.Mitchell: "Regulation by gonadal steroids of the mRNA encoding for a type I receptor for TGF-β in the female rat hypothalamus"Neuroendocrinology. 76・1. 1-7 (2002)

S.Bouret、V.Prevot、T.Takumi、JC.Beauvillain、V.Mitchell：“性腺类固醇对雌性大鼠下丘脑中 TGF-β I 型受体编码的 mRNA 的调节”神经内分泌学 76・1。 1-7 (2002)

Y. Shigeyoshi, E. Meyer-Bernstein, K. Yagita,W. Fu, Y. Chen, T. Takumi, P. Schotland,A. Sehgal and H. Okamura: "Restoration of circadian behavioural rhythms in a period null Drosophila mutant (per^0) by mammalian period homologues mPer1 and mPer2."Genes C

Y. Shigeyoshi、E. Meyer-Bernstein、K. Yagita、W.

S. Bouret, V. Prevot, T. Takumi.JC. Beauvillain and V. Mitchell: "Gonadal Steroids regulation of the mRNA encoding for a type I receptor for TGFβ in the female rat hypothalamus."Neuroendocrinology. 76-1. 1-7 (2002)

S. Bouret、V. Prevot、T. Takumi.JC. Beauvillain 和 V. Mitchell：“性腺类固醇对雌性大鼠下丘脑中 TGFβ 受体编码的 mRNA 的调节”。神经内分泌学 76-1。 7 (2002)