Integrative analyses of circadian rhythms based on environment

我们使用多种高通量的方法，包括染色质免疫沉淀（ChIP）为基础的系统分析和DNA微阵列结合生物信息学，以产生全基因组的BMAL1靶基因的配置文件。我们发现，除了E盒，BMAL1识别的共识序列CCAATG引起强大的昼夜节律表达。BMAL1占据了150多个位点，包括所有已知的时钟基因。重要的是，BMAL1靶点中很大一部分包括编码代谢过程的中央调节因子的基因。本研究中产生的数据库构成了破译昼夜节律基因控制网络及其与几种基本生理功能的密切联系的有用资源

We use multiple high-throughput approaches including chromatin immunoprecipitation(ChIP)-based systematic analyses and DNA microarrays combined with bioinformatics, to generate genome-wide profiles of BMAL1 target genes. We reveal that, in addition to E-boxes, BMAL1 recognizes a consensus sequence CCAATG to elicit robust circadian expression. BMAL1 occupancy is found in more than 150 sites, including all known clock genes. Importantly, a significant proportion of BMAL1 targets include genes that encode central regulators of metabolic processes. The database generated in this study constitutes a useful resource to decipher the network of circadian gene control and its intimate links with several fundamental physiological functions