Development of new method for early detection & diagnosis of adenoma malignum of the uterin cervix

开发早期检测新方法

• 批准号: 13670168
• 负责人: ISHII Keiko
• 金额: $ 0.7万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670168/
• 关键词: uterine cervix; adenoma malignum; HIK1083; latex agglutination test; gland mucus cell type mucin; α4GnT; RT-PCR; 子宮頸部; 悪性腺種

1. We evaluated the usefulness of a rapid, simple method for the diagnosis of adenoma malignum (AM) and related lesions using a latex agglutination test. Cervical mucus samples from 120 patients including 10 gastric-type cervical lesions and 5 positive controls were included in this study. We employed a latex agglutination test kit developed by Kanto Chemical. Clear agglutination confirmed its specificity was observed in only 10 gastric-type cervical lesions and all positive controles. It has demonstrated that the HIK1083-labeled latex agglutination test is highly specific for the identification of gastric mucin in the cervical mucus. This method enables us to diagnose AM and related lesions at their clinically early stage.2. We evaluated the usefulness of the RT-PCR assay targeted to α 1,4-N-Acetylglucosaminyltransferase (α4GnT) for the detection of AM and lerated lesions, α 4GnT is a glycosyltransferase that forms a unique glycan, specifically present in gastric gland mucous cell-type mucin. To detect gastric type cancer cells circulating in the peripheral blood of AM and related lesions, we quantitatively analyzed the expression level of α4GnT mRNA in the mononuclear cell fraction of peripheral blood using RT-PCR. The transcripts of α4GnT were detected in the mononuclear cell fraction isolated from all patients but not from any of 23 healthy individuals. These results altogether indicate that the quantitative analysis of α4GnT mRNA expressed in the peripheral blood is useful for the detection and, possibly, monitoring of AM and related lesions as well as gastric cancer.

1.我们评估了一种快速，简单的方法诊断腺瘤（AM）和相关病变使用乳胶凝集试验的有用性。本研究包括120例患者的宫颈粘液样本，包括10例胃型宫颈病变和5例阳性对照。我们采用了由关东化学开发的乳胶凝集试验试剂盒。明确的凝集证实其特异性仅在10个胃型宫颈病变和所有阳性对照中观察到。已证明HIK 1083标记的乳胶凝集试验对于鉴定宫颈粘液中的胃粘蛋白具有高度特异性。这种方法使我们能够在临床早期诊断AM和相关病变。我们评价了靶向α 1，4-N-乙酰氨基葡萄糖转移酶（α4GnT）的RT-PCR检测方法在AM和变应性病变检测中的有效性。α 4GnT是一种糖基转移酶，可形成独特的聚糖，特异性存在于胃腺粘液细胞型粘蛋白中。为检测AM及相关病变外周血中的胃癌细胞，采用RT-PCR方法定量分析AM患者外周血单个核细胞中α4GnT mRNA的表达水平。α4GnT的转录本在从所有患者中分离的单核细胞组分中检测到，但在23名健康个体中未检测到。结果表明，定量分析外周血中α4GnT mRNA的表达，对AM及其相关病变的检测和胃癌的监测有一定的意义。

项目成果

Shimizu Fumiaki: "Usefulness of the real-time reverse transcription-polymerase chain reaction assay targeted to α1,4-N acetylglucosaminyltrass ferase for the detection of gastric cancer"Lab Invest. 83. 187-197 (2003)

Shimizu Fumiaki：“针对 α1,4-N 乙酰氨基葡萄糖酶的实时逆转录聚合酶链式反应测定在检测胃癌中的用途”Lab Invest。 83. 187-197 (2003)

Keiko Ishii: "Histo-and cytochemistry of gland mucus cell type muchin and its practical application for pathologic and clinical diaqnosis"Acta Histochem Cytochem. 35. 48 (2002)

Keiko Ishii：“muchin 型腺体粘液细胞的组织和细胞化学及其在病理和临床诊断中的实际应用”Acta Histochem Cytochem。

Fumiaki Shimizu: "Usefulness of the real-time reverse transcription-polymerase chain reaction assay targeted to α1,4-Nacetylglucosaminyltransferase for the detection of Gastric Cancer"Laboratory Inerstigation. 83. 187-197 (2003)

Fumiaki Shimizu：“针对 α1,4-N 乙酰葡糖胺基转移酶的实时逆转录聚合酶链式反应测定在检测胃癌中的用途”Laboratory Inerstigation 83. 187-197 (2003)。

Keiko Ishii: "Histo- and cytochemistry of gland mucus cell type mucin and its practical application for pathologic and clinical diagnosis"Acta Histochem cytochem. 35. 48 (2002)

Keiko Ishii：“腺体粘液细胞型粘蛋白的组织和细胞化学及其在病理和临床诊断中的实际应用”Acta Histochem cytochem。