Signal Transduction relevant to Cherno-klinokinesis of schistosome miracidiai toward intermediate snail host

血吸虫向中间蜗牛宿主的切尔诺运动相关的信号转导

• 批准号: 13670251
• 负责人: AOKI Yoshiki
• 金额: $ 2.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670251/
• 关键词: schistosome; miracidium; secretion of snail; chemo-klinokinesis; chemotaxis; lectin; WGA; cAMP; 感覚器; ケモ・クリノカイネシス; 情報伝達

Schistosorne miracidia show chemo-klinokinesis toward the secretions of the intermediate snails (Snail Conditioned Water : SCW). A certain kinds of nematode larvae are reported to loose their chennotactic activity when their sensory papillae are overspread by some kinds of lectin. Miracidia must recognize SCW by the sensory papillae on their body surface. The present study was attempted to show the presence of receptor on the miracidia by using lectin binding. Materials and Method : Miracidia were incubated with any hind of lectin. They were then examined for their swimming behavior and for cheinotactic action toward SCW. Lectin used was WGA, PNA, GS-II and MPA. Results and Discussion : Of lectin examined, only WGA, at high concentration (100 ug/ml), caused the behavior, change (frequent change in swimming direction). And miracidia pre-incubated with WGA, at 250 ug/ml for 10 min, did not show the cherotactic action toward SCW. These results indicate that 1) the receptors of SCW are pre … More sent on the body surface of miracidia, and 2) miracidia loose chemotactic action when the receptors are overspread by WGA. Recently we reported that CAMP induces the frequent change in swimming direction of miracidia as well as SCW does. Then an attempt was made to examine whether WGA inhibits cAMP-induced miracidial behavior change. Miracidia pre-incubated with WGA showed the frequent change of swimming direction. Since this result suggests the role of cAMP as a 2^<nd> messenger of the signal transduction of chemo-klinokinesis of miracidia, an attempt was made to verify our hypothesis. Miracidia were incubated with the reagents that lower the intercellular e_AMP concentration (MDL-12,330A, SQ 22526, imidazole) or inhibitor of protein kinase A (PKI(14-22) amid), and then examined for their chemotactic action toward SCW. As was expected, these miracidia did reduce the chemotacitc activity toward SCW. These results clearly indicate that cAMP plays an important role in chemo-klinokinesis. of miracidia toward SCW. Less

血吸虫毛蚴对中间型蜗牛的分泌物（蜗牛调理水：SCW）显示化学趋化作用。某些线虫幼虫的感觉乳头被某些凝集素覆盖后，其趋化性丧失。毛蚴必须通过体表的感觉乳头识别SCW。本研究试图用凝集素结合的方法来证明毛蚴上受体的存在。材料与方法：毛蚴与外源凝集素孵育。然后检查它们的游泳行为和对SCW的趋化作用。所用凝集素为WGA、PNA、GS-Ⅱ和MPA。结果和讨论：在检测的凝集素中，只有高浓度（100 μ g/ml）的WGA引起行为改变（游泳方向的频繁改变）。250 μ g/ml的WGA预孵育毛蚴10 min，毛蚴对SCW无定向作用。这些结果表明：（1）超临界水的受体是前体细胞， ...更多信息 2）毛蚴在WGA的覆盖下失去趋化作用。最近，我们报道了CAMP和SCW一样，能引起毛蚴游动方向的频繁改变。然后尝试检查WGA是否抑制cAMP诱导的毛蚴行为改变。毛蚴经WGA预孵育后，游动方向发生频繁变化。由于这一结果提示cAMP在毛蚴化学<nd>趋化的信号转导中起着2^-信使的作用，因此我们试图验证我们的假设。将毛蚴与降低细胞内e_AMP浓度的试剂（MDL-12、330 A、SQ 22526、咪唑）或蛋白激酶A抑制剂（PKI（14-22）amid）孵育，观察它们对SCW的趋化作用。正如预期的那样，这些毛蚴确实降低了对SCW的趋化活性。这些结果清楚地表明cAMP在化学运动中起重要作用。毛蚴向SCW发展。少

项目成果

Matsuyama H., Aoki Y.et al.: "The involvement of cyclic adenosine monophosphate in the control of Schistosome miracidium cilia"Journal of Parasitology. 90. 8-14 (2004)

Matsuyama H.、Aoki Y.等人：“环磷酸腺苷参与控制血吸虫纤毛”寄生虫学杂志。