Identification of virulent factors related to rearrangement of skeleton Proteins in mycobacterial infections.

鉴定与分枝杆菌感染中骨架蛋白重排相关的毒力因子。

• 批准号: 13670289
• 负责人: KIRIKAE Teruo
• 金额: $ 1.92万
• 依托单位: Research Institute, International Medical Center of Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670289/
• 关键词: Mycobacterium tuberculosis; Rhodococcus equi; Macrophage; TACO; ロドコッカク エクイ; BCG

To identify new virulent factors of intracellular bacteria, such as Mycobacterium tuberculosis and Rhodococcus equi, murine macrophage-like J774. 1 cells were infected with virulent or avirulent R. equi and observed by using immunocytological methods. Observations by light microscopy and transmission electron microscopy showed that the virulent bacteria increased into single membrane vacuoles. Observations by confocal fluorescence microscopy with an endosomal marker and lysosomal markers revealed that most phagosomes containing virulent bacteria were fused with lysosomes. To monitor acidification of phagosomes containing R. equi, the cells infected with R. equi were stained with acidotropic probe. The phagosomes containing avirulent R. equi became acidic. In contrast, virulent R. eqiu did not change pH. The medium of virulent R. equi was suppressed acidification of acidic compartments in macrophages. These results suggested that virulent R. equi can survive phagolysosomal environment in macrophages by secreting substance which suppress acidification of phagolysosome.

为了鉴定细胞内细菌的新有毒因素，例如结核分枝杆菌和Equi，鼠类巨噬细胞样J774。 1个细胞用毒或无毒的R. Equi感染，并使用免疫细胞学方法观察到。通过光学显微镜和透射电子显微镜观察表明，毒细菌增加到单膜液泡中。与内体标记和溶酶体标记物通过共聚焦荧光显微镜观察表明，大多数含有毒细菌的吞噬体与溶酶体融合在一起。为了监测含有R. equi的吞噬体的酸化，用酸性探针染色了。含有无毒性的R. equi的吞噬体变成酸性。相反，有毒的R. Eqiu没有改变pH。毒性R. Equi的培养基被抑制巨噬细胞中酸性室的酸化。这些结果表明，毒性菌Equi可以通过分泌抑制吞噬体酸化的物质来在巨噬细胞中生存。

项目成果

Funatogawa, K., et al.: "Use of Immunoglobulin Enriched Bovine Colostrum against Oral Challenge with Enterohaemorrhagic Escherichia coli O157:H7 in Mice"Microbiol. Immunol.. 46. 761-766 (2002)

Funatokawa, K. 等人：“使用富含免疫球蛋白的牛初乳对抗小鼠肠出血性大肠杆菌 O157:H7 的口服攻击”微生物学。

Hashimoto, H., et al.: "Structural study on lipid A and the 0-specific polysaccharide of the lipopolysaccharide from a clinical isolate Bacteroides vulgatus from a patient with Crohn's disease., 269:3715-3721, 2002."Eur. J. Biochem.. 269. 3715-3721 (2002)

Hashimoto, H. 等人：“对来自克罗恩病患者临床分离的普通拟杆菌的脂质 A 和脂多糖的 0 特异性多糖的结构研究。”，269：3715-3721，2002 年。

Watando, A. et al.: "Mycobacterium avium infection in an immunocompetent young adult related to use of home bath with a circulating water system"Jpn. J. Infect. Dis.. 54. 151-152 (2001)

Watando, A. 等人：“免疫功能健全的年轻人中与使用带有循环水系统的家庭浴室有关的鸟分枝杆菌感染”Jpn。

Takahara, M., et al.: "Pulmonary Mycobacterium avium infection in an immunocompetent aged woman related to use of home bath with a circulating water system"Jpn. J. Infect. Dis.. 55. 213-214 (2002)

Takahara, M., et al.：“免疫功能正常的老年妇女的肺分枝杆菌感染与使用带有循环水系统的家庭浴室有关”Jpn。