The biochemical features and functions of NS gene product of influenza C virus

丙型流感病毒NS基因产物的生化特征及功能

• 批准号: 13670293
• 负责人: HONGO Seiji
• 金额: $ 2.24万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670293/
• 关键词: Influenza C virus; NS gene; NS2; virion; incorporation

Recently we identified the 182 amino acid NS2 protein as a protein of 〜22kDa in influenza C virus-infected cells. In this study, it was demonstrated that the NS2 protein of influenza C virus was present in purified virions as follows.1. Western blot analysis with anti-NS2 serum detected the NS2 protein in egg-grown influenza C/Yamagata/1/88 virions purified by centrifugation through a linear gradient of 5%-40% potassium tartrate. NS2 was also present either in purified virions of other strains grown in eggs or in purified C/Yamagata/1/88 virions grown in other host cells(MDCK or HMV-II).2. The NS2 protein was detected in influenza C virions purified by using the binding activity either to the rat red blood cells or to anti-Hemagglutinin-esterase(HE) monoclonal antibody-conjugated protein A sepharose.3. The NS2 protein associated with influenza C virions purified by centrifugation through a linear gradient of 5%-40% potassium tartrate was completely resistant to the treatment with 200 μg/ml of TPCK-trypsin for 30 min at 37℃.4. These data indicate that the NS2 protein of influenza C virus is present inside the viral envelope.

最近，我们在丙型流感病毒感染细胞中发现了182个氨基酸的NS2蛋白，其大小约为22 kDa。本研究证明丙型流感病毒的NS2蛋白存在于纯化的病毒粒子中，如下所示。用5%-40%酒石酸钾线性梯度离心法纯化的鸡蛋生长的流感病毒C/Yamagata/1/88病毒粒子，用抗NS2血清进行Western印迹分析，检测到NS2蛋白。NS2也存在于其他毒株的纯化病毒粒子中，或存在于其他宿主细胞(MDCK或HMV-II)中的纯化C/Yamagata/1/88病毒粒子中。利用与大鼠红细胞或抗血凝素-酯酶(HE)单抗结合蛋白A琼脂糖凝胶的结合活性，在纯化的C型流感病毒粒子中检测到NS2蛋白。经5%-40%酒石酸钾线性梯度离心纯化的与C流感病毒粒子相关的NS2蛋白对200μg/mlTPCK-胰酶在37℃作用30min完全耐受。这些数据表明，C型流感病毒的NS2蛋白存在于病毒被膜内。

项目成果

Muyanga J. et al.: "Antigenic and genetic analyses of influenza B viruses isolated in Lusaka, Zambia in 1999"Arch. Virol.. 146. 1667-1679 (2001)

Muyanga J. 等人：“1999 年在赞比亚卢萨卡分离的乙型流感病毒的抗原和遗传分析”Arch.

Muyanga, J.: "Antigenic and genetic analyses of influenza B viruses isolated in Lusaka,Zambia in 1999"Archives of Virology. 46・9. 1667-1679 (2001)

Muyanga, J.：“1999 年在赞比亚卢萨卡分离的乙型流感病毒的抗原和遗传分析”病毒学档案 46・9 (2001)。

Tsuchiya E. et al.: "Antigenic structure of the haemagglutinin of human influenza A/H2N2 virus."J. Gen. Virol.. 82. 2475-2484 (2001)

Tsuchiya E.等人：“人甲型流感/H2N2流感病毒血凝素的抗原结构”。

Tsuchiya E. et al.: "Antigenic structure of the haemagglutinin of human influenza A/H2N2 virus"J. Gen. Virol.. 82. 2475-2484 (2001)

Tsuchiya E.等：“人甲型流感/H2N2流感病毒血凝素的抗原结构”J.

Tsuchiya E. et al.: "Role of overlapping glycosylation sequons in antigenic properties, intracellular transport and biological activities of influenza A/H2N2 virus haemagglutmin"J. Gen. Virol.. 83. 3067-3074 (2002)

Tsuchiya E.等人：“重叠糖基化序列在甲型/H2N2流感病毒血凝素的抗原特性、细胞内转运和生物活性中的作用”J.