Studies on the ion channel activity of influenza C virus CM2 protein

丙型流感病毒CM2蛋白离子通道活性研究

基本信息

  • 批准号:
    11670287
  • 负责人:
  • 金额:
    $ 2.43万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

The sites for fatty acylation, disulfide bond formation and phosphorylation of influenza C virus CM2 were investigated by site-specific mutagenesis. Cysteine 65 in the cytoplasmic tail was identified as the site for palmitoylation. Removal of one or more of three cysteine residues in the ectodomain showed that all of cysteines 1, 6, and 20 can participate in the formation of disulfide- linked dimers and/or tetramers, although cysteine 20 may play the most important role in tetramer formation. Furthermore, it was found that serine 78, located within the recognition motifs for mammary gland casein kinase and casein kinase I, is the predominant site for phosphorylation, although serine 103 is phosphorylated to a minor extent by proline -dependent protein kinase. The effects of acylation and phosphorylation on the formation of disulfide-linked oligomers were also studied. The results showed that, while palmitoylation has no role in oligomer formation, phosphorylation accelerates tetramer formation without influencing dimer formation. CM2 mutants defective in acylation, phosphorylation or disulfide bond formation were all transported to the cell surface, suggesting that none of these modifications is required for proper oligomerization. When proteins solubilized in detergent were analysed on sucrose gradients, however, the mutant lacking cysteines 1, 6 and 20 sedimented as monomers, raising the possibility that disulfide bond formation, although not essential for proper oligomerization, may stabilize the CM2 multimer. This was supported by the results of chemical cross-linking analysis which showed that the triple cysteine mutant can form multimers.
用定点突变的方法研究了C型流感病毒CM2的脂肪酰化、二硫键形成和磷酸化的位点。胞质尾部的半胱氨酸65被确定为棕榈酰化的位点。胞外结构域中三个半胱氨酸残基中的一个或多个的去除表明,所有的半胱氨酸1、6和20都可以参与二硫键连接的二聚体和/或四聚体的形成,尽管半胱氨酸20可能在四聚体的形成中发挥最重要的作用。此外,研究还发现,位于乳腺酪蛋白激酶和酪蛋白激酶I识别基序中的丝氨酸78是主要的磷酸化位点,尽管丝氨酸103被脯氨酸依赖的蛋白激酶有少量的磷酸化。还研究了酰化和磷酸化对二硫键低聚物形成的影响。结果表明,棕榈酰化对低聚物的形成没有影响,而磷酸化促进了四聚体的形成,而不影响二聚体的形成。在酰化、磷酸化或二硫键形成方面存在缺陷的CM2突变体都被运输到细胞表面,表明这些修饰都不是适当的寡聚所必需的。然而,当对溶解在洗涤剂中的蛋白质进行蔗糖梯度分析时,缺乏半胱氨酸1、6和20的突变体以单体形式沉淀,这增加了二硫键形成的可能性,尽管二硫键的形成对适当的齐聚不是必需的,但可能稳定CM2多聚体。化学交联分析结果表明,三重半胱氨酸突变体可以形成多聚体。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Matsuzaki Y., Mizuta K., Kimura H., Sugawara K., Tsuchiya E., Suzuki H., Hongo S., Nakamura K.: "Characterization of antigenically unique influenza C virus strains isolated in Yamagata and Sendai Cities, Japan, during 1992-1993."J.Gen. Virol.. 81(6). 1447
Matsuzaki Y.、Mizuta K.、Kimura H.、Sukawara K.、Tsuchiya E.、Suzuki H.、Hongo S.、Nakamura K.:“在日本山形市和仙台市分离的抗原独特的丙型流感病毒株的表征,
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    0
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Muraki Y., Hongo S., Sugawara K., Matsuzaki Y., Takashita E., Kitame F., Nakamura K.: "Location of a linear epitope recognized by monoclonal antibody S16 on the hemagglutinin-esterase glycoprotein of influenza C virus."Virus Res.. 61(1). 53-61 (1999)
Muraki Y.、Hongo S.、Sukawara K.、Matsuzaki Y.、Takashita E.、Kitame F.、Nakamura K.:“丙型流感病毒血凝素酯酶糖蛋白上单克隆抗体 S16 识别的线性表位的位置。
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    0
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Matsuzaki,Y.: "Characterization of the antigenically unique influenza C strains isolated in Yamagata and Sendai Cities, Japan during 1992/1993"J.Gen.Virol.. 81・6. 1447-1452 (2000)
Matsuzaki, Y.:“1992/1993 年日本山形市和仙台市分离的抗原性独特的丙型流感病毒株的特征”J.Gen.Virol.. 81・6 (2000)。
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Matsuzaki Y.: "Characterization of the antigenically unique influenza C strains isolated in Yamagata and Sendai Cities,Japan during 1992/1993"J Gen Virol. (in press).
Matsuzaki Y.:“1992/1993 年在日本山形市和仙台市分离的抗原性独特的丙型流感病毒株的特征”J Gen Virol。
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    0
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Alamgir,A.S.M.: "Phylogenetic analysis of influenza C virus nonstructual (NS) protein genes and identification of the NS2 protein"J.Gen.Virol.. 81. 1933-1940 (2000)
Alamgir,A.S.M.:“丙型流感病毒非结构 (NS) 蛋白基因的系统发育分析和 NS2 蛋白的鉴定”J.Gen.Virol.. 81. 1933-1940 (2000)
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HONGO Seiji其他文献

HONGO Seiji的其他文献

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{{ truncateString('HONGO Seiji', 18)}}的其他基金

The role of CM2 ion channel protein in influenza C virus replication and pathogenesis
CM2离子通道蛋白在丙型流感病毒复制和发病机制中的作用
  • 批准号:
    20590465
  • 财政年份:
    2008
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The effect of the regulatory mechanism of splicing on influenza C virus replication
剪接调控机制对丙型流感病毒复制的影响
  • 批准号:
    17590413
  • 财政年份:
    2005
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The biochemical features and functions of NS gene product of influenza C virus
丙型流感病毒NS基因产物的生化特征及功能
  • 批准号:
    13670293
  • 财政年份:
    2001
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The biosynthesis mechanism of influenza C virus CM2 protein and its ion channel activity
丙型流感病毒CM2蛋白的生物合成机制及其离子通道活性
  • 批准号:
    09670307
  • 财政年份:
    1997
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Characterization of a protein candidate for the ion channel of influenza C virus and its expression.
丙型流感病毒离子通道候选蛋白的表征及其表达。
  • 批准号:
    07670337
  • 财政年份:
    1995
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Effect of cytoplasmic tail of CM2 protein on influenza C virus replications
CM2蛋白胞质尾部对丙型流感病毒复制的影响
  • 批准号:
    16K19136
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Involvement of the CM2 ion channel activity in the influenza C virus replication cycle
CM2离子通道活性参与丙型流感病毒复制周期
  • 批准号:
    24590560
  • 财政年份:
    2012
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of functional domains of influenza C virus CM2 protein involved in viral replication
丙型流感病毒CM2蛋白参与病毒复制的功能域的鉴定
  • 批准号:
    23790494
  • 财政年份:
    2011
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Functional analysis of CM2 in influenza C virus replication by using the mutants of the posttranslational modification sites
利用翻译后修饰位点突变体分析CM2在丙型流感病毒复制中的功能
  • 批准号:
    22790439
  • 财政年份:
    2010
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
MRI: Development of Molecular Beam Expitaxy Apparatus for Growth of Two Dimensional Electron Gas Systems with Mobility of 100,000,000 cm2/Vs
MRI:开发用于生长迁移率达 100,000,000 cm2/Vs 的二维电子气系统的分子束外延装置
  • 批准号:
    0923369
  • 财政年份:
    2009
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Standard Grant
Role of CM2 in the influenza C virus replication cycle
CM2 在丙型流感病毒复制周期中的作用
  • 批准号:
    21590505
  • 财政年份:
    2009
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of CM2 ion channel protein in influenza C virus replication and pathogenesis
CM2离子通道蛋白在丙型流感病毒复制和发病机制中的作用
  • 批准号:
    20590465
  • 财政年份:
    2008
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
FUNCTIONS OF THE INFLUENZA C VIRUS CM2 AND P31 PROTEINS
C 型流感病毒 CM2 和 P31 蛋白的功能
  • 批准号:
    6012922
  • 财政年份:
    1999
  • 资助金额:
    $ 2.43万
  • 项目类别:
C型インフルエンザウイルスCM2蛋白の翻訳後修飾部位の決定
丙型流感病毒CM2蛋白翻译后修饰位点的测定
  • 批准号:
    09770198
  • 财政年份:
    1997
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Encouragement of Young Scientists (A)
The biosynthesis mechanism of influenza C virus CM2 protein and its ion channel activity
丙型流感病毒CM2蛋白的生物合成机制及其离子通道活性
  • 批准号:
    09670307
  • 财政年份:
    1997
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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