权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis for volatile organic compounds by cryogenic oven trapping capillary gas chromatography

低温烘箱捕集毛细管气相色谱法分析挥发性有机化合物

基本信息

批准号：
13670420
负责人：
WATANABE Kanako
金额：
$ 2.18万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Recently, a microcomputer-controlled device for lowering oven temperature below 0℃ has become available for new types of gas chromatography (GC) instruments. This device was originally designed for rapid cooling of an oven to reduce the time for analysis. In our laboratories, we are using it for trapping volatile organic compounds (VOCs) inside a capillary column at cryogenic oven temperatures ; as much as 5 mL of headspace vapor can be injected into a medium-bore capillary columun without any loss, giving sensitivity 10-100 times higher than that by the conventional headspace GC method. In addition to the above high sensitivity, much better resolution (separation) of compounds can be also achieved, probably because VOCs are trapped at a cryogenic oven temperature in a quite narrow zone of the front part of a capillary column.The method is recommendable for wide use in forensic and environmental toxicology, because it is simple and requires no special GC operations in addition to the above high sensitivity and high resolution. We named the method cryogenic oven trapping with capillary gas chromatography (COT-GC).We had studied analysis of some important volatile organic compounds (VOCs) such as chloroform, dichloromethane, trichloroethylene, diethyl ether, the components of solvent thinner, xylene isomers, cyanide, ethanol, hexane, general anesthetics in human body fluids, and we had got good results and had published analysis methods in many international journals of toxicology.In this research project, we especially studied new methods for analysis of styrene and related compounds, alkyl alcohol as decomposition products of alkyl nitrites and amphetamines with derivatization in human body fluids with COT-GC. We have presented each detailed procedure for their sensitive assays in some international journals of toxicology.

最近，一种微电脑控制的将炉温降低到0℃以下的装置已经可以用于新型的气相色谱(GC)仪器。该设备最初是为快速冷却烤箱而设计的，以减少分析时间。在我们的实验室中，我们正在使用它在低温下将挥发性有机化合物(VOCs)捕获到毛细管柱中；多达5毫升的顶空蒸汽可以无损失地注入中等口径的毛细管柱，使灵敏度比传统的顶空GC方法高10-100倍。除了上述高灵敏度外，可能是因为VOCs被捕获在低温烘箱温度下的毛细管柱前部相当窄的区域内，所以该方法还可以实现更好的分辨(分离)。该方法简单，除了上述高灵敏度和高分辨率外，不需要特殊的GC操作，因此推荐在法医学和环境毒理学中广泛使用。我们将这一方法命名为毛细管气相色谱(CoT-GC)，对人体体液中的氯仿、二氯甲烷、三氯乙烯、二乙醚、溶剂稀释剂成分、二甲苯异构体、氰化物、乙醇、正己烷、全身麻醉剂等重要挥发性有机化合物进行了分析，取得了较好的效果，并在许多国际毒理学期刊上发表了分析方法。我们已经在一些国际毒理学期刊上详细介绍了他们的敏感分析方法。