Development of automated dynamic headspace extraction method for determination of halomethanes and application to bacterial culture samples

开发用于测定卤代甲烷的自动动态顶空萃取方法及其在细菌培养样品中的应用

• 批准号: 20510013
• 负责人: HASHIMOTO Shinya
• 金额: $ 3.08万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20510013/
• 关键词: ハロカーボン; 微生物; 色素分析; 共存系; 動態解析; ダイナミックヘッドスペース法; ガスクロマトグラフ質量分析装置; バクテリア; イソプレン; 植物プランクトン; 共存; 微量ガス; GC-MS; 共存培養

Halomethane produced in the marine environment are thought to play a key role in atmospheric reactions, particularly those involved in the global radiation budget and the depression of tropospheric and stratospheric ozone. To evaluate halomethane concentrations in the bacterial culture samples, we developed an automated dynamic headspace extraction method for the determination of 15 halomethanes. The halomethanes in the gas phase above the culture samples were measured using dynamic headspace (GESTEL DHS)-gas chromatograph (Agilent 6890N)- mass spectrometer (Agilent 5975C). Productions of halomethane were observed for several days.

据认为，海洋环境中产生的卤代甲烷在大气反应中发挥关键作用，特别是在全球辐射收支和对流层及平流层臭氧降低中发挥关键作用。为了评估细菌培养样品中卤代甲烷的浓度，我们开发了一种自动化动态顶空萃取法用于测定15种卤代甲烷。使用动态顶空（GESTEL DHS）-气相色谱仪（Agilent 6890 N）-质谱仪（Agilent 5975 C）测量培养物样品上方的气相中的卤代甲烷。几天来一直观察到卤代甲烷的产生。

项目成果

Production of volatile organic compounds in cultures of cryptophytes

隐植物培养物中挥发性有机化合物的产生

• 发表时间: 2010
• 作者: T.Yamakoshi;M.Kurihara;S.Hashimoto
• 通讯作者: S.Hashimoto

Production and air-sea flux of methyl halides in the western subarctic Pacific in relation to phytoplankton pigment concentrations during iron fertilization experiment(SEEDS II)

亚北极西太平洋卤代甲烷的产生和海气通量与铁施肥实验中浮游植物色素浓度的关系(SEEDS II)

• 发表时间: 2009
• 期刊: Deep Sea Research II
• 作者: S. Hashimoto;S. Toda;K. Suzuki;S. Kato;Y. Narita;M. K. Kurihara;Y. Akatsuka;H. Oda;T. Nagai;I. Nagao;I. Kudo;M. Uematsu
• 通讯作者: M. Uematsu

Optimization of dynamic headspace extraction system for measurement of halogenated volatile organic compounds in liquid or viscous samples

用于测量液体或粘性样品中卤代挥发性有机化合物的动态顶空萃取系统的优化

• 发表时间: 2010
• 作者: G.Taniai;H.Oda;M.Kurihara;S.Hashimoto
• 通讯作者: S.Hashimoto

DHS-GC/MS分析法によるCH_3Brを含むハロカーボンの測定

DHS-GC/MS分析法测定CH_3Br等卤代烃

• 发表时间: 2010
• 作者: 谷合元;ら
• 通讯作者: ら

Distributions of short-lived iodocarbons and biogenic trace gases in the open ocean and atmosphere in the western North Pacific

• DOI: 10.1016/j.marchem.2009.12.001
• 发表时间: 2010-02
• 期刊: Marine Chemistry
• 影响因子: 3
• 作者: M. Kurihara;M. Kimura;Yoko Iwamoto;Y. Narita;A. Ooki;Y.-J. Eum;A. Tsuda;Koji Suzuki;Y. Tani-Y.-T