Identification of endothelium-derived hyperpolarizing factor (EDHF) in human arteries.

人体动脉中内皮源性超极化因子（EDHF）的鉴定。

• 批准号: 13670724
• 负责人: HIRAKAWA Yoji
• 金额: $ 2.24万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670724/
• 关键词: EDHF; Vascular endothelial cells; EDRFs; Hydrogen peroxide; 内皮依存性過分極因子; ブラジキニン; カタラーゼ; ギャップジャンクション

We have recently identified that endothelium-derived hydrogen peroxide (H202) is an endothelium-derived hyperpolarizing factor (EDHF) in mice (Journal of Clinical Investigation, 2002). In this series of experiments, we have examined whether H202 is also and EDHF in human arteries.In isolated human mesenteric arteries, EDHF-mediated responses were inhibited by catalase that specifically decomposes H202 into water and oxygen. Exopgenous H202 also exerted relaxations and hyperpolarizations of vascular smooth muscle. By contrast, we were unable to confirm other hypotheses on the nature of EDHF, including epoxyeicosatrienoic acids (EETs), K ion, and gap junctions.These results indicate that H202 is also an EDHF in human mesenteric arteries.

我们最近已经鉴定出内皮衍生的过氧化氢（H2O2）是小鼠中的内皮衍生的超极化因子（EDHF）（Journal of Clinical Investigation，2002）。在这一系列的实验中，我们检测了H2O2是否也是人动脉中的EDHF，在离体的人肠系膜动脉中，EDHF介导的反应被特异性地将H2O2分解成水和氧的过氧化氢酶抑制。外源性H_（202）对血管平滑肌也有舒张和超极化作用。与此相反，我们无法证实其他关于EDHF性质的假设，包括环氧二十碳三烯酸（EHF）、K离子和间隙连接。

项目成果

Matoba T, et al.: "Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in human mesenteric arteries"Biophys Biochem Res Comm.. 290. 909-913 (2002)

Matoba T 等人：“过氧化氢是人肠系膜动脉中内皮衍生的超极化因子”Biophys Biochem Res Comm.. 290. 909-913 (2002)

Yada T, et al.: "Rho-kinase mediates hypoxia-induced downregulation of endothelial nitric oxide synthase"Circulation. 107. 1040-1045 (2003)

Yada T 等人：“Rho 激酶介导缺氧诱导的内皮一氧化氮合酶下调”循环。

Matoba T, et al,: "Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in human mesenteric arteries."Biophyc Biochem Res Commun.. 290. 909-913 (2002)

Matoba T 等人：“过氧化氢是人肠系膜动脉中内皮衍生的超极化因子。”Biophyc Biochem Res Commun.. 290. 909-913 (2002)

Yada T, et al.: "Hydrogen peroxide, an endogenous EDHF, plays an important role in coronary autoregulation in vivo"Circulation. (in press). (2003)

Yada T 等人：“过氧化氢是一种内源性 EDHF，在体内冠状动脉自动调节中发挥着重要作用”。

Tetsuya Matoba: "Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in human mesenteric arteries"Biochemical and Biophysical Research Communication. 290. 909-913 (2002)

Tetsuya Matoba：“过氧化氢是人类肠系膜动脉中内皮衍生的超极化因子”生物化学和生物物理研究通讯。