Effect of high shear stress-dependent platelet-derived microparticle for the interaction of monocyte and endothelial cell

高剪切应力依赖性血小板衍生微粒对单核细胞与内皮细胞相互作用的影响

• 批准号: 13670760
• 负责人: NOMURA Shosaku
• 金额: $ 2.18万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670760/
• 关键词: PDMP; MAPK; PKC; soluble cell adhesion molecule; chemokine; endothelial cell; type 2 diabetes mellitus; acute coronary syndrome; 高ずり応力; サイトカイン; プロテインキナーゼC; MAPキナーゼ; 単球; CD40L

We investigated the effect of high shear stress-dependent platelet-derived microparticle(PDMP) for the interaction of monocyte and endothelial cell. IL-6, TPO and EPO enhanced the generation of high shear stress-dependent PDMP. PDMP enhanced the ICAM-1 expression of endothelial cell, and PKC activation and COX-2 induction were observed in this event. In addition, the activation of p38, JNK1, and MAPK were observed in monocytic cell line(THP-1). On the other hand, the activation of PKC and MAPK at endothelial cell and THP-1 was suppressed by the anti-CD40L antibody. These results suggested PDMP generated by high shear stress caused the CD40L expression effected by cytokines, and enhanced the expression of cell adhesion molecules in vascular endothelial cell and monocyte in PKC/MAPK dependence. The relation between PDMP, chemokine, and soluble cell adhesion molecules in patients with diabetes mellitus and ischemic heart disease. The PDMP and soluble cell adhesion molecules in hypertensive patients with diabetes mellitus were significantly increased compared to that without diabetes mellitus. In addition, these parameters were obviously increased in the hyperlipidemic patients with diabetes mellitus.

我们研究了高剪切应力依赖性血小板衍生微粒（PDMP）对单核细胞和内皮细胞相互作用的影响。 IL-6、TPO 和 EPO 增强了高剪切应力依赖性 PDMP 的生成。 PDMP增强了内皮细胞的ICAM-1表达，并且在此事件中观察到PKC激活和COX-2诱导。此外，在单核细胞系（THP-1）中观察到p38、JNK1和MAPK的激活。另一方面，内皮细胞和THP-1处的PKC和MAPK的活化被抗CD40L抗体抑制。这些结果表明，高剪切应力产生的PDMP引起细胞因子影响的CD40L表达，并增强血管内皮细胞和单核细胞中PKC/MAPK依赖性细胞粘附分子的表达。糖尿病和缺血性心脏病患者PDMP、趋化因子和可溶性细胞粘附分子之间的关系。高血压合并糖尿病患者的PDMP和可溶性细胞粘附分子较非糖尿病患者显着升高。此外，这些参数在糖尿病合并高脂血症的患者中明显升高。

项目成果

Matsumoto N, et al.: "Association of platelet-derived microparticles with C-C chemokines on vascular complication in patients with acute coronary syndrome"Clin Appl Thrombosis/Hemostasis. 8. 279-286 (2002)

Matsumoto N 等人：“血小板衍生微粒与 C-C 趋化因子对急性冠脉综合征患者血管并发症的关联”Clin Appl 血栓/止血。

Nomura S, et al.: "Effects of eicosapentaenoic acid on platelet activation markers and cell adhesion molecules in hyperlipidemic patients with type 2 diabetes mellitus"Journal of Diabetes and its Complications. (in press). (2001)

Nomura S等人：“二十碳五烯酸对患有2型糖尿病的高脂血症患者的血小板活化标志物和细胞粘附分子的影响”糖尿病及其并发症杂志。

Matsumoto N, et al.: "Association of platelet-derived microparticles with C-C chemokines on vascular complication in patients with acute myocardial infarction"Clin Appl Thrombosis/Hemostasis. 8. 279-286 (2002)

Matsumoto N 等人：“血小板衍生微粒与 C-C 趋化因子对急性心肌梗死患者血管并发症的关联”Clin Appl 血栓/止血。

Matsumoto N, et al.: "Associatlon of platelet-derived microparticles with C-C chemokines on vascular complication in patients with acute myocardial infarction"Clin Appl Thrombosis / Hemostasis. 8. 279-286 (2002)

Matsumoto N 等人：“血小板衍生微粒与 C-C 趋化因子对急性心肌梗塞患者血管并发症的关联”Clin Appl 血栓/止血。

Nomura S, et al.: "Effects of Saiko-ka-ryukotsu-borei--to in patients with hyperlipidemia"Phytomecicine. 8. 165-173 (2001)

Nomura S 等人：“Saiko-ka-ryukotsu-borei 对高脂血症患者的影响”植物药。