New method of evaluation and diagnosis for DIC using endothelial cell-derived microparticles

使用内皮细胞微粒评估和诊断 DIC 的新方法

• 批准号: 23590694
• 负责人: NOMURA Shosaku
• 金额: $ 3.49万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590694/
• 关键词: 血管内皮細胞由来マイクロパーティクル; HMGB1; リコンビナントトロンボモジュリン; SIRS; DIC; 造血器腫瘍; EDMP; TNFα; CD144; TF; L-selectin; Mac-1; MAPK; PKC

We found that, although TRAP-stimulated platelets could induce up-regulation of some molecules such as CD40, CD80, CD83, CD86 and HLA-DR to some extent, the addition of MPs further increased this expression compared with expression induced by TRAP-stimulated platelets. In addition, the enhancement of expression of these molecules in response to TRAP-stimulated platelets or MPs was completely inhibited by anti-CD154 antibody. For DIC patients with hematologic malignancy, univariate analysis showed that PT, fibrinogen, CRP, PDMP, EDMP, sCD40L, IL-6, TNF-alpha, RANTES and MCP-1 were significantly associated with HMGB1. DIC patients exhibited significant decrease in HMGB1 levels after rTM therapy. In addition, the levels of platelet activation markers and cytokines were significantly decreased after rTM therapy in DIC patients. Our findings indicate the role of EDMP and HMGB1 in DIC patients with hematologic malignancy.

我们发现，尽管TRAP刺激的血小板可以在一定程度上诱导CD40、CD80、CD83、CD86和人类白细胞抗原-DR等分子的表达上调，但与TRAP刺激的血小板相比，MPS的加入进一步增加了这种表达。此外，抗CD154抗体可完全抑制这些分子在TRAP刺激的血小板或MPS刺激下的表达增强。单因素分析显示，PT、Fg、CRP、PDMP、EDMP、sCD40L、IL-6、TNF-α、RANTES、MCP-1与HMGB1显著相关。DIC患者经RTM治疗后HMGB1水平显著降低。此外，DIC患者经RTM治疗后，血小板活化标志物和细胞因子水平显著降低。我们的研究结果提示EDMP和HMGB1在血液系统恶性肿瘤患者DIC中的作用。

项目成果

An intimate link between dendritic cells and platelets in the maintenance of allergic response

树突状细胞和血小板在维持过敏反应中的密切联系

• 发表时间: 2012
• 作者: Takahisa Nakanishi;Tomoki Ito;Muneo Inaba;Shousaku Nomura
• 通讯作者: Shousaku Nomura

造血器疾患と止血凝固(第1回) 造血幹細胞移植に関連した凝固異常病態

造血系统疾病与凝血（一）造血干细胞移植相关的凝血异常

• 发表时间: 2013
• 期刊: Thrombosis Medicine
• 作者: 湊宏;他;野村昌作
• 通讯作者: 野村昌作

多発性骨髄腫と凝固線溶異常

多发性骨髓瘤与凝血和纤溶异常

• 发表时间: 2013
• 期刊: Thrombosis Medicine
• 作者: 藤田真也;野村昌作
• 通讯作者: 野村昌作

DICの診断基準と治療薬の知見

DIC诊断标准及治疗药物知识

• 发表时间: 2013
• 期刊: 日本内科学会雑誌
• 作者: Kawamura Y;Sugata K;Ihira M;Mihara T;Mutoh T;Asano Y;Yoshikawa T;野村昌作
• 通讯作者: 野村昌作

マイクロパーティクルの測定と標準化

微粒测量和标准化

• 发表时间: 2012
• 期刊: 細胞
• 作者: 野村 昌作;藤田 真也;玉置 岳史;東 秀二;永井 豊
• 通讯作者: 永井 豊