The screaning of endothelial function of young adult patients with Kawasaki disease history

有川崎病史的青年患者内皮功能筛查

• 批准号: 13670826
• 负责人: SAKATA Koichi
• 金额: $ 0.77万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670826/
• 关键词: Kawasaki disease; endothelial function; athersclerosis; screening; NO関連因子

Objects: It is suspected that Kawasaki disease (KD) is a risk factor pre-mature of atherosclerosis, because atherosclerosis is regarded as a post-inflammatory disease. However, there is clinical evidence of the hypothesis. To clarify the above, it is necessary to establish an easy and reproducible screening. Therefore, we evaluated %FMD, PWV, ADMA+SDMA/L-ARG and NOX (NO2- + NO3-) as endothelial function and NO related factors in young adult patients with KD history. Method: (1) %FD: the diameter of brachial arteries at rest and durion reactive hyperemia was measured using high-resolution ultrasound, (2) PWV: using from PWN/ABI (Kolin Inc, Japan), (3) plasma ADMA, SDMA and L-ARG: using HPLC, (4) NOX: using capillary electrophoresis (CE). Results and Discussion: (1) (2) both %FMD and PWV were significantly elevated in the patients with coronary arterial lesions (CAL). There was no significant correlation between both of them. It was suspected that %FMD is more sensitive than PWV, though PWV was rather easier and more reproducible than %FMD. (3) Plasma ADMA, SDMA and L-ARG could be detected under the specific condition for RF-HPLC using fluoreccence label, but under an ordinary condition for UV detection. (4) Plasma or urine NOX: NOX could be detected using OFM buffer based CE at 185nm. Unfortunately, we could not get enough data about KD patients in our schedule, because we spent too much time to establish the trustworthy detecting-condition for these biochemical factors. We still proceed this study, and plan to present the detail results in near future

目的：由于动脉粥样硬化被认为是一种炎症后疾病，故认为川崎病(KD)是动脉粥样硬化的危险因素。然而，有临床证据证明了这一假设。为了澄清上述问题，有必要建立一种简单且可重复性的筛查方法。因此，我们评估%FMD、PWV、ADMA+SDMA/L-精氨酸和NOX(NO_2+NO_3-)是有KD病史的年轻成人患者的内皮功能，而不是相关因素。方法：(1)%FD：采用高分辨率超声测量静息状态和硬膜反应性充血时的肱动脉内径；(2)PWV：采用日本科林公司生产的PWV；(3)血浆ADMA、SDMA和L精氨酸：采用高效液相色谱法；(4)NOX：采用毛细管电泳法。结果与讨论：(1)(2)冠状动脉病变(CAL)患者的%FMD和PWV均显著升高。两者之间没有显著的相关性。有人怀疑%FMD比PWV更敏感，尽管PWV比%FMD更容易和更具重复性。(3)荧光标记法在特定条件下能检测到血浆ADMA、SDMA和L-精氨酸，而在普通UV检测条件下检测不到。(4)基于OFM缓冲液的CE在185 nm波长处可以检测到血浆或尿液中的NOx：NOx。不幸的是，我们无法在我们的时间表上获得足够的KD患者的数据，因为我们花费了太多的时间来建立这些生化因素的可靠检测条件。我们仍在进行这项研究，并计划在不久的将来公布详细的结果

项目成果

坂田耕一: "川崎病血管炎におけるhHGFとMMP-9の関与"Progress in Medicine. 21(7). 1669-1675 (2001)

Koichi Sakata：“hHGF 和 MMP-9 在川崎病血管炎中的参与”医学进展 21(7) 1669-1675 (2001)。