The screaning of endothelial function of young adult patients with Kawasaki disease history

对象：由于动脉粥样硬化被认为是一种炎症后疾病，因此川崎（KD）被认为是动脉粥样硬化的一个危险因素。然而，有临床证据证明了这一假设。为了澄清上述问题，有必要建立一个简单和可重复的筛选。因此，我们评估了%FMD、PWV、ADMA+SDMA/L-ARG和NOX（NO2- + NO3-）作为有KD病史的年轻成人患者的内皮功能和NO相关因素。方法：（1）%FD：用高分辨率超声测量静息和持续性反应性充血时的肱动脉直径，（2）PWV：使用PWN/ABI（Kolin Inc，Japan），（3）血浆ADMA、SDMA和L-ARG：使用HPLC，（4）NOX：使用毛细管电泳（CE）。结果与讨论：（1）（2）冠状动脉病变（CAL）患者的%FMD和PWV均显著升高。两者之间无显著相关性。尽管PWV比%FMD更容易且更可重复，但怀疑%FMD比PWV更敏感。(3)荧光标记的RF-HPLC法在特定条件下可检测血浆中的ADMA、SDMA和L-ARG，而紫外检测法在常规条件下可检测血浆中的ADMA、SDMA和L-ARG。(4)血浆或尿液中的NOX：NOX可以使用基于OFM缓冲液的CE在185 nm处检测。然而，由于我们花费了大量的时间来建立这些生化指标的可靠检测条件，我们无法在我们的计划中获得足够的关于KD患者的数据。我们仍在进行这项研究，并计划在不久的将来提出详细的结果

Objects: It is suspected that Kawasaki disease (KD) is a risk factor pre-mature of atherosclerosis, because atherosclerosis is regarded as a post-inflammatory disease. However, there is clinical evidence of the hypothesis. To clarify the above, it is necessary to establish an easy and reproducible screening. Therefore, we evaluated %FMD, PWV, ADMA+SDMA/L-ARG and NOX (NO2- + NO3-) as endothelial function and NO related factors in young adult patients with KD history. Method: (1) %FD: the diameter of brachial arteries at rest and durion reactive hyperemia was measured using high-resolution ultrasound, (2) PWV: using from PWN/ABI (Kolin Inc, Japan), (3) plasma ADMA, SDMA and L-ARG: using HPLC, (4) NOX: using capillary electrophoresis (CE). Results and Discussion: (1) (2) both %FMD and PWV were significantly elevated in the patients with coronary arterial lesions (CAL). There was no significant correlation between both of them. It was suspected that %FMD is more sensitive than PWV, though PWV was rather easier and more reproducible than %FMD. (3) Plasma ADMA, SDMA and L-ARG could be detected under the specific condition for RF-HPLC using fluoreccence label, but under an ordinary condition for UV detection. (4) Plasma or urine NOX: NOX could be detected using OFM buffer based CE at 185nm. Unfortunately, we could not get enough data about KD patients in our schedule, because we spent too much time to establish the trustworthy detecting-condition for these biochemical factors. We still proceed this study, and plan to present the detail results in near future