THE ANALYSIS OF CHROMOSOMAL GAINS AND LOSSES IN PRIMARY MALIGNANTMELANOMA-COMPARISON WITH SPITZ NEVUS-

原发性恶性黑色素瘤染色体增益和缺失的分析-与斯皮茨痣的比较-

• 批准号: 13670875
• 负责人: MATSUMOTO Kazuhiko
• 金额: $ 0.26万
• 依托单位: SHINSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670875/
• 关键词: MELANOMA; SPITZ NEVUS; MICRODISSECIION; COMPARATIVE GENOMIC HYBRIDIZATION

SPITZ NEVUS IS A BENIGN NEOPLASM OF MELANOCYTES THAT CAN BE DIFFICULT OR IMPOSSIBLE TO DISTINGUISH FROM MELANOMA BY CLINICAL AND HISTOPATHOLOGIC EXAMINATION. WE TRIED TO FIND CHROMOSOMAL GAIN AND LOSSES BY COMPARATIVE GENOMIC HYBRIDIZATION (CGH) IN FORMALIN-FIXED, PARAFFIN ENMBEDDED TISSUES FROM MELANOMA, AND COMPARE WITH THAT FROM SPITZ NEVUS. HOWEVER, THE SIZE OF DNA EXTRACTED FROM FORMARIN-FIXED, PARAFFIN-EMBEDDED TISSUE IS TOO SMALL FOR CGH. THEREFORE, WE FIRST TRIED TO ESTABLISH THE CGH METHOD USING MELANOMA CELL LINE, MMG1. REFERENCE DNA WAS EXTRATED FROM NORMAL FEMALE PERIPHERAL BLOOD LYMPHOCYTES. WE GOT THE FOLLOWING RESULTS.1. EXTRACTION OF DNA : DNA EXTRACTOR WB KIT (WAKO, OSAKA) CAN BE USED FOR THE EXTRACTION FROM THE CELL LINES.2. LABELING TIME : ONE AND A HALF HOUR FOR MMG1 CELLS. THREE HOURS FOR PERIPHERAL BLOOD LYMPHOCYTES.3. PRECIPITATION TIME WITH ETHANOL : -80℃, ONE HOUR4. HYBRIDIZATION TIME: 37℃, 3 DAYSWE HAVE ESTABLISHED THE CGH METHOD IN OUR LABORATORY THE FINAL PROBLEM IS THE DNA EXTRACTION FROM FORMARIN-FIXED, PARAFFIN-EMBEDDED TISSUES. WE WILL SOLVE THE PROBLEM SOON AND WE CAN FIND CHROMOSOMAL GAIN AND LOSSES BY COMPARATIVE GENOMIC HYBRIDIZATION (CGH) IN FORMALIN-FIXED, PARAFFIN ENMBEDDED TISSUES FROM MELANOMA AND SPITZ NEVUS.

斯皮茨痣是一种良性黑素细胞肿瘤，临床和组织病理学检查很难或不能与黑色素瘤鉴别。我们尝试用比较基因组杂交（CGH）技术，在石蜡包埋的石蜡包埋组织中，从黑色素瘤中寻找染色体的增益和损失，并与Spitz痣的染色体进行比较。然而，从福尔马林固定、石蜡包埋的组织中提取的DNA的大小对于CGH来说太小了。因此，我们首先尝试用黑色素瘤细胞系MMG 1建立CGH方法。从正常雌性外周血淋巴细胞中提取参考DNA。我们得到了以下证据。DNA的提取：DNA Extractor WB Kit（和子，OSAKA）可用于细胞系的提取。2.标记时间：MMG 1细胞为一个半小时。三个小时的外周血淋巴细胞。乙醇沉淀时间：-80℃，1小时4。混合时间：37℃，3天，我们已经建立了CGH方法在我们的实验室，最后的问题是从福尔马林固定，石蜡包埋组织中提取DNA。我们将很快解决这个问题，我们可以通过比较基因组杂交（CGH）在福尔马林固定的石蜡包埋的黑色素瘤和斯皮茨痣组织中发现染色体的增益和损失。

项目成果

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FUKUZAWA M: "A CASE OF MALIGNANT MELANOMA WITH PARTIAL RFGRESSION OF PRIMARY LESION AND VITILIGO"RINNSHO HIFUKA. 56. 245-248 (2002)

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松本和彦: "左拇趾球部に生じた小型の悪性黒色腫"Visual Dermatology. 1. 40-41 (2002)

Kazuhiko Matsumoto：“左拇指球部的小恶性黑色素瘤”《Visual Dermatology》1. 40-41 (2002)。

福沢正男: "白斑を合併し、原発巣の部分退縮を示した悪性黒色腫の1例"臨床皮膚科. 56. 245-248 (2002)

Masao Fukuzawa：“恶性黑色素瘤并发白癜风并显示原发肿瘤部分消退的病例”《临床皮肤病学》56. 245-248 (2002)。

KAGESHITA T: "LOSS OF β-CATHENIN EXPRESSION ASSOCIATED WITH DISEASE PROGRESSION IN MALIGNANT MELANOMA"BRITISH JOURNAL OF DERMATOLOGY. 145. 210-216 (2002)

KAGESHITA T：“β-Cathenin 表达的丧失与恶性黑色素瘤的疾病进展相关”，《英国皮肤病学杂志》145. 210-216 (2002)。