Developmental changes in cerebral optical parameters in neonates using near-infrared time-resolved spectroscopy

使用近红外时间分辨光谱研究新生儿脑光学参数的发育变化

• 批准号: 13671140
• 负责人: ISOBE Kenichi
• 金额: $ 1.6万
• 依托单位: Kanagawa University (Faculty of Medicine)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671140/
• 关键词: near-infrared spectroscopy; time-resolved spectroscopy; piglet; new born infant; differential pathlength factor; light reduced scattering coefficient; cerebral hemoglobin oxygen saturation; cerebral blood volume; 低出生体重児; 近赤外時間分解分光測定; 散乱係数; 吸光係数

We used a portable three-wavelength near-infrared time-resolved spectroscopy (TRS) system to measure the light absorption coefficient (μa), light reduced scattering coefficient (μ's), hemoglobin (Hb) concentration and Hb oxygen saturation (SO2) in the brain. In a basic study using a piglet hypoxic model, the relationship between differential pathlength factor (DPF) and hemoglobin oxygenation state in the brain was investigated. In a clinical study using neonates, the developmental changes in those parameters were investigated.(1)Measurement of cerebral light pathlength by TRS in a piglet model of hypoxiaWe investigated the relationships of mean light pathlength with arterial (SaO2) and sagittal sinus venous Hb oxygen saturation (SvO2) in the brain under the condition of stepwise hypoxia loading. The ratio of mean light pathlength to interoptode distance is defined as the DPF. At FiO2 of 0.21, the DPF values at 761,795 and 835 nm were estimated to be 5.02±0.36, (mean±SD), 5.31±0.32 and 4.92±0.34,respectively. There was a significant positive linear correlation between DPF at 761 and 795 nm and SaO2. The DPF and SvO2 also showed a significant positive linear correlation at 761 and 795 nm. The factors influencing the DPF were increase in deoxyHb and decrease in oxyHb during hypoxia.(2)Measurement of cerebral optical parameter,802 and cerebral blood volume by TRS in the developing brainThe subjects were 19 neonates whose gestational age was 25 to 41 weeks. The DPF values at 761,795 and 835 nm were estimated to be 4.9±0.52,5.02±0.53 and 4.64±0.46,respectively. The values of μ's at each wavelength were 6.30±1.30,6.04±1.11 and 6.37±1.45/cm respectively. The CBV was 2.2±0.6 ml/100g, which is similar to the value previously reported. The SO2 was 73±4.0% under 95-100% of SpO2. The SO2 and postconceptual weeks showed a significant negative linear relationship. The reason for this is the increase in cerebral oxygen consumption with development of the brain.

采用便携式三波长近红外时间分辨光谱仪（TRS）测量了脑组织的光吸收系数（μa）、光约化散射系数（μ s）、血红蛋白（Hb）浓度和血红蛋白氧饱和度（SO2）。在基础研究中，使用小猪缺氧模型，差分路径长度因子（DPF）和血红蛋白氧合状态之间的关系在脑中进行了研究。在一项使用新生儿的临床研究中，研究了这些参数的发育变化。（1）TRS法测定缺氧模型仔猪脑组织光程长度在逐步低氧负荷条件下，研究了脑组织平均光程长度与动脉血氧饱和度（SaO_2）和矢状窦静脉血红蛋白血氧饱和度（SvO_2）的关系。平均光路长度与尖端间距离的比值被定义为DPF。当FiO 2为0.21时，在761、795和835 nm处的DPF值分别为5.02±0.36、5.31±0.32和4.92± 0.34。761和795 nm处的DPF与SaO 2呈显著正相关。DPF和SvO 2在761和795 nm处也表现出显著的正线性相关。缺氧时影响DPF的因素是deoxyHb增加和oxyHb减少。（2）TRS法测定新生儿脑发育过程中的脑光学参数、802和脑血容量。在761、795和835 nm处的DPF值分别为4.9± 0.52、5.02 ±0.53和4.64± 0.46。各波长的μ s值分别为6.30± 1.30，6.04 ±1.11和6.37±1.45/cm。CBV为2.2±0.6 ml/100 g，与文献报道的CBV值相似。在SpO 2为95-100%时，SO2为73±4.0%。SO2与受孕后周数呈显著负线性关系。其原因是随着大脑的发育，大脑耗氧量增加。

项目成果

Isobe K, Kusaka T, Fujikawa Y, Okubo K, Nagano K, Yasuda S, Kondo M, Itoh S, Hirao K, Onishi S: "Measurement of cerebral oxygenation in neonates after vaginal delivery and cesarean section using full-spectrum infrared spectroscopy"Comp Biochem Phys. A132.

Isobe K、Kusaka T、Fujikawa Y、Okubo K、Nagano K、Yasuda S、Kondo M、Itoh S、Hirao K、Onishi S：“使用全光谱红外光谱测量阴道分娩和剖腹产后新生儿的脑氧合”

Kusaka T, Kawada K, Okubo K, Nagano K, Namba M, Okada H, Imai T, Isobe K, Itoh S: "Noninvasive optical imaging in the visual cortex in young infants"

Kusaka T、Kawada K、Okubo K、Nagano K、Namba M、Okada H、Imai T、Isobe K、Itoh S：“小婴儿视觉皮层的非侵入性光学成像”

Kusaka T, Hisamatsu Y, Kawada K, Okubo K, Okada H, Namba M, Imai T, Isobe K, Itoh S: "Measurement of Cerebral Optical Pathlength as a Function of Oxygenation Using Near-infrared Time-resolved Spectroscopy in a Piglet Model of Hypoxia"Opt Rev. 10. 466-469

Kusaka T、Hisamatsu Y、Kawada K、Okubo K、Okada H、Namba M、Imai T、Isobe K、Itoh S：“在仔猪模型中使用近红外时间分辨光谱测量脑光程长度作为氧合的函数

Isobe K, Kusaka T, Fujikawa Y, Okubo K, Nagano K, Yasuda S, Kondo M, Itoh S, Hirao K, Onishi S: "Measurement of cerebral oxygenation in neonates after vaginal delivery and cesarean section using full-spectrum near infrared spectroscopy"Comp Biochem Phys A

Isobe K、Kusaka T、Fujikawa Y、Okubo K、Nagano K、Yasuda S、Kondo M、Itoh S、Hirao K、Onishi S：“使用全谱近红外光谱仪测量阴道分娩和剖腹产后新生儿的脑氧合

Kusaka T, Okubo K, Nagano K, Yasuda S, Kawada K, Imai T, Isobe K, Itoh S: "Activation of the visual cortex in newborn infants under natural sleep using multichannel near-infrared spectroscopy"Adv Exp Med Biol. 510. 255-259 (2003)

Kusaka T、Okubo K、Nagano K、Yasuda S、Kawada K、Imai T、Isobe K、Itoh S：“使用多通道近红外光谱激活自然睡眠下新生儿的视觉皮层”Adv Exp Med Biol。