Biochyperhemical evaluation of isolated porcine liver system perfused with oxygenated blood by ECMO

ECMO灌注含氧血离体猪肝系统的生物热化学评价

• 批准号: 13671262
• 负责人: IKAWA Hiromiti
• 金额: $ 2.3万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671262/
• 关键词: Liver transplantation; perfusion preservation; hyperglyicemia

Aim : The optimal condition of liver perfusion was investigated in order to prolong the preservation time of liver graft and also to avoid primary non-function or re-perfusion injury.Materials and Methods : Twenty one porcine livers were purfused with oxygenated blood by ECMO, using venous-venous bypass via portal vein to infrahepatic IVC. After supra IVC and hepatic artery were ligated, thliver was harvested and kept on perfusing in 37℃ lactate Ringer solution.Results : Since some amount of bile juice was excreted throughout the experiment, biological activity of the liver grafts seem to be maintained essentially by meaning of perfusion with oxygenated blood for more than 12 hours. However, it was made clear that the oxygenated perfusion via portal vein be responsible for severe metabolic and biochemical problems. They were hyponatremia, metabolic asidosis and hyperglycemia which were resistant to insulin administration.

目的：材料与方法：21例猪肝经体外膜肺氧合（ECMO）氧合血灌注，经门静脉转流至肝下下腔静脉（IVC）。结果：由于在实验过程中有一定量的胆汁排出，含氧血灌流12 h以上，移植肝的生物活性基本维持。然而，经门静脉的氧合灌注是造成严重代谢和生化问题的原因。低钠血症、代谢性酸中毒和高血糖是胰岛素抵抗的表现。

项目成果

生体部分肝移植周術期における細菌感染とその対策

活体部分肝移植围术期细菌感染及其对策

• 发表时间: 2003
• 期刊: 日小外会誌 39
• 作者: 増山 宏明;伊川廣道;et
• 通讯作者: et

乳幼児生体部分肝移植の問題点と対策

婴儿活体部分肝移植存在的问题及对策

• 发表时间: 2002
• 期刊: 日小外会誌 38
• 作者: 増山 宏明;伊川廣道;et
• 通讯作者: et

Problems and measures of reduced-size orthotropic liver Transplantation in children.

儿童减体积正交异性肝移植的问题与措施。

• 发表时间: 2002
• 期刊: J.J Soc Pediatr.Surg 38
• 作者: H Masuyama;H Ikawa
• 通讯作者: H Ikawa

Optical timing of liver transplantation and quality of life in the child with biliary atresia.

肝移植的光学时机和胆道闭锁儿童的生活质量。

• 发表时间: 2001
• 期刊: J.J Soc Pediatr.Surg 37
• 作者: H Okajima;S Okamoto;H Ikawa
• 通讯作者: H Ikawa

Bacterial infection in peri-operative period in the children operated on reduced-size orthotropic liver transplantation.

儿童减体积正交异性肝移植围术期细菌感染的分析

• 发表时间: 2003
• 期刊: J.J Soc Pediatr.Surg 39
• 作者: H Masuyama;H Ikawa
• 通讯作者: H Ikawa