Pre-clinical study on pulmonary perfusion preservation using modified ET-Kyoto solutions

使用改良 ET-Kyoto 溶液保存肺灌注的临床前研究

• 批准号: 23592056
• 负责人: BANDO TORU
• 金额: $ 3.33万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592056/
• 关键词: 呼吸器外科; 移植・再生医療; トランスレーショナルリサーチ; 外科

We evaluated the effects of continuous perfusion on pulmonary preservation using a rat and a dog ex vivo perfusion models aiming at clinical application of modified ET-K solution as a perfusate. Neither a potassium-reduced ET-K nor an ET-K without HES demonstrated sufficient pulmonary preservation for reliable clinical practice. In addition, supplementation with red blood cells to the perfusion solution also failed to show any significant improvements of the preservation effect. A further extensive modification is needed for the clinical application.

为探讨改良ET-K液作为肺保存液的临床应用价值，我们采用大鼠和犬的体外肺灌流模型，评价了持续灌流对肺保存的影响。无论是去钾的ET-K组还是不含HES的ET-K组，对于可靠的临床实践都没有足够的肺保护作用。此外，在灌注液中添加红细胞也没有显示出任何明显的保存效果改善。临床应用还需要进一步的大范围改进。