Experimental study for reconstruction of portal vein and inferior vena cava in liver surgery

肝脏手术中门静脉及下腔静脉重建的实验研究

• 批准号: 13671343
• 负责人: YAMAGUCHI Masahiko
• 金额: $ 1.92万
• 依托单位: Dokkyo University School of Medicine (2002)Showa University (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671343/
• 关键词: reconstruction of IVC; hepatectomy; hepatocyte growth factor; ecdothelial seeding

Results in 2001: Adenovirus vector containing hepatocyte growth factor cDNA (AdHGF) was propagated in 293 cells and concectrated with purification. Vascular endothelial cells (ECs) were isolated from canine jugular vein and cultured in M199+10%FCS. Cytotoxicity and HGF production were investigated to determine the most effective dose of AdHGF. As a result, it was concluded that 50 PFU of AdHGF per cell were the most effective dose.Results in 2003: Replacement of IVC with PTFE graft where AdHGF-transfected ECs were seeded was performed using 5 dogs after right hepatectomy. Two out of 5 dogs were survived, but three other dogs were dead within a week after the operation. Causes of death included untolerable damages and bleeding by the operation. Survived two dogs were sacrificed 4 weeks after the operation to investigate the liver regeneration and seeds grafts. There was no clot adherent to the graft where viable ECs were attached. Liver regeneration of hepatectomized dogs with seeded IVC graft was not different from hepatectomized dogs with unseeded graft. Serum concentrations of HGF were measured in the operation. However, levels of HGF in all serum samples were less than measurable ones. It is suggested that HGF expression levels from AdHGF-transfected ECs on the graft might not be enough to show biological activity.

结果：2001年，携带肝细胞生长因子基因(AdHGF)的腺病毒载体在293细胞中扩增，纯化后浓缩。从犬颈静脉分离血管内皮细胞，在M199+10%胎牛血清中培养。研究AdHGF的细胞毒性和HGF的产生，以确定AdHGF的最有效剂量。结果：2003年，5只犬右肝切除后，用携带AdHGF基因的血管内皮细胞种植的PTFE移植物替代下腔静脉。5只狗中有2只活了下来，但另有3只狗在手术后一周内死亡。死因包括手术造成的无法忍受的损伤和出血。存活的两只犬于术后4周处死动物，观察肝再生情况及种子移植情况。移植物上没有凝块附着在可存活的内皮细胞上。带种子下腔静脉的肝切除犬与未植入种子的切除肝犬肝再生无明显差异。术中检测血清HGF浓度。然而，所有血清样本中的HGF水平都低于可测量的水平。提示AdHGF转导的内皮细胞在移植物上的HGF表达水平可能不足以显示其生物学活性。

项目成果

M.Yamaguchi, H.Nemoto, et al.: "Effects of Insulin-like growth factor-1 on short bowel syndrome"Eur Serg Res. 33. 291-296 (2001)

M.Yamaguchi、H.Nemoto 等人：“胰岛素样生长因子-1 对短肠综合征的影响”Eur Serg Res。

Min Li, Genshu Tate, Masahiko Yamaguchi, et al.: "Hepatocellular Carcinoma in a Patient with Acromegaly and High Serum Levels of Insulin-like Growth Factor I: Report of a Case"Showa Univ J Med Sci. 14. 169-173 (2002)

Min Li、Genshu Tate、Masahiko Yamaguchi 等：“肢端肥大症和高血清胰岛素样生长因子 I 水平患者的肝细胞癌：病例报告”Showa Univ J Med Sci。

山口真彦, 浅川清人, 高木悦子, 中島政博, 根本洋, 真田裕, 熊田馨: "回盲部合併小腸大量切除に対する実験的検討:成分栄養、IGF-1投与の効果について"外科と代謝・栄養. 36. 49-54 (2002)

Masahiko Yamaguchi、Kiyoto Asakawa、Etsuko Takagi、Masahiro Nakajima、Hiroshi Nemoto、Yutaka Sanada、Kaoru Kumada：“大规模小肠切除术联合回盲部的实验研究：成分营养和 IGF-1 给药的影响”手术与代谢。营养。 36. 49-54 (2002)

Okuyama T, Oya M, Yamaguchi Ml: "Budding(sprouting)as a useful prognostic marker in colorectal mucinous carcinoma"Jpn J Clin Oncol. 32. 412-416 (2002)

Okuyama T、Oya M、Yamaguchi Ml：“出芽（发芽）作为结直肠粘液癌的有用预后标志物”Jpn J Clin Oncol。

山口真彦, 吉澤康男, 緑川武正, 他: "重症壊死性膵炎後の膵仮性嚢胞に対する胃瘻を介した外瘻術の1例"外科. 63・1. 106-109 (2001)

山口正彦、吉泽康夫、绿川武正等：“重症坏死性胰腺炎后经胃造口术治疗胰腺假性囊肿的一例”Surg. 106-109。