The role of tissue factor on lung cancer metastasis

组织因子在肺癌转移中的作用

• 批准号: 13671371
• 负责人: MINAMIYA Yoshihiro
• 金额: $ 2.56万
• 依托单位: AKITA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671371/
• 关键词: non-small lung cancer; tissue factor; metastasis; inavasion; blood vessel; lymphatic vessel; 血管浸潤

Purpose; Tissue factor (TF), an initiator of the extrinsic coagulation cascade, is also expressed in a wide range of cancer cells and plays important roles in cancer progression, and metastasis, processes independent of the blood coagulation pathway. For example, by acting as an adhesion molecule enabling tissue invasion, TF may play a key role in the metastatic process and angiogenesis in non-small cell lung cancer (NSCLC). Methods: To further investigate the role of TF on tumor cell invasion in NSCLC, we measured TF mRNA expression in the tumors of 42 NSCLC patients using real time quantitative reverse transcription polymerase chain reaction carried out in a LightCycIer. We then compared TF mRNA expression with the histological evidence of invasion of blood and lymphatic vessels by tumor cells. Results: Although, there was no significant relationship between TF mRNA expression and invasion of lymphatic vessels, TF mRNA expression was significantly higher in tumors that invaded blood vessels (Log_<10 >TF mRNA/GAPDH mRNA = 2.16 ± 0.18) than in those that did not (1.59 ± 0.16, P = 0.03). Conclusion: These results suggest that TF plays a major role in blood vessel invasion by tumor cells in NSCLC.

目的;组织因子（TF）是外源性凝血级联反应的启动子，也在广泛的癌细胞中表达，并在癌症进展和转移中起重要作用，这些过程独立于血液凝固途径。例如，通过充当使组织侵入成为可能的粘附分子，TF可在非小细胞肺癌（NSCLC）的转移过程和血管生成中发挥关键作用。研究方法：为进一步探讨TF在非小细胞肺癌侵袭中的作用，我们采用真实的实时定量逆转录聚合酶链反应（RT-PCR）技术检测了42例非小细胞肺癌组织中TF mRNA的表达。然后，我们比较TF mRNA表达与肿瘤细胞侵袭血管和淋巴管的组织学证据。结果如下：TF mRNA的表达与淋巴管浸润无关，但有血管浸润的肿瘤TF mRNA的表达（LogTF <10 >mRNA/GAPDH mRNA = 2.16 ± 0.18）显著高于无血管浸润的肿瘤（1.59 ± 0.16，P = 0.03）。结论：TF在NSCLC的血管侵袭中起重要作用。