The new type of receptor for antithrombin III that has anti-inflammatory effect

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Many reports indicated that antithrombin III is effective for not only disseminated intravascular coagulation syndrome but also sepsis. This suggests that antithrombin III has not only anticoagulatic action but also anti-inflammatory effect. The aim of this study is to find the new receptor for antithrombin III which take an anti-inflammatory action. It has been assumed that one of the glycosaminoglycan could be a receptor for antithrombin III. However, in this study we demonstrated that after cleaving heparin sulfate on the surface of the neutrophil with heparinase, antithrombin III binds to the neutrophil. This suggests that there must be a non-glycosaminoglycan receptor for antithrombin III. According to this hypothesis, we started to search the non-glycosaminoglycan receptor for antithrombin III. We searched for a kind of protein on the surface of the tumor cell, HT1080, binds to antithrombin III with mass spectrograph, after cleaving heparin sulfate on the surface of HT1080 with heparinase and sodium chlorate. However, unlike our first expectation, we found that antithrombin III directly binds to the core protein of syndecan-4 that belongs to glycosaminoglycan. The core protein of syndecan-4 acts as a totally new type of receptor for antithrombin III.