Studies on the invasive potential of malignant gliomas

恶性胶质瘤侵袭潜力的研究

• 批准号: 13671469
• 负责人: NAGASHIMA Tadashi
• 金额: $ 1.6万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671469/
• 关键词: invasion; fast blue; HGF; MIB-1SI; P53; GEAP; fibronectin; グリオーマ; 多発性腫瘍; 弧発性腫瘍; MIB-1; ファイブロネクチン; ラミニン; ヌードラット; 蛍光染色; トグリオーマ株細胞; 腫瘍細胞浸潤; U251MG; U87MG

5×10^5U87MG cells stained with fast blue were inoculated in to the right basal ganglia of nude rat (F344/Njcl-mu). Five and 10 days after inoculation, hepatocytic growth factor (HGF) was injected into the brain of each nude rat. Two to three weeks after tumor inplantation, the brain of each nude rat were excised, fixed, and cut into 6 micro mm frozen sections.We observed the brain tumor and its surrounding brain tssue of each frozen section with fluorescein microscope. We found that tumor cells were invaded into the sorrounding brain tissue markedly as compared with control section which prepared from the nude rat without HGF administration. This result suggests that HGF increase the invasive potential of glial tumor cells.We investigated the biological characteristics such as proliferative potential estimated from MIB-1 SI, and expression of P53 GFAP, fibronectin of multicentric glioblastomas, and compare them with those of singlecentric glioblastomas. In this study, paraffin enbedded sections of four multicentric glioblastomas and five singlecentric glioblastomas were used for immunohistochemical staining of MIB-1, P53, GEAP and fibronectin. The value of MIB-1SI and the degree of expression of P53, GFAP, fibronectin appere to be no significant difference between multicentric glioblastomas and singlecentric glioblastomas. These results suggest that some other intrinsic factors, such as MMP-2 and VEGF, may participate in invasion of glioblastomas.

将5×10^5U87MG细胞接种于裸鼠（F344/Njcl-mu）右侧基底节区。接种后第5天和第10天，将肝细胞生长因子（HGF）注射到每只裸大鼠的脑中。于肿瘤植入后2 ~ 3周，切除裸鼠脑组织，固定，制成6 μ mm冰冻切片，荧光显微镜下观察肿瘤及其周围脑组织。我们发现，与未注射HGF的裸大鼠制备的对照切片相比，肿瘤细胞明显侵入周围脑组织。本研究通过MIB-1 SI检测多中心性胶质母细胞瘤的增殖能力、P53、GFAP、FN的表达等生物学特性，并与单中心性胶质母细胞瘤进行比较。本研究应用免疫组织化学方法对4例多中心性胶质母细胞瘤和5例中央性胶质母细胞瘤进行了MIB-1、P53、GEAP和纤维连接蛋白的染色。MIB-1 SI值及P53、GFAP、FN的表达在多中心性胶质母细胞瘤与多中心性胶质母细胞瘤之间无显著性差异。提示MMP-2、VEGF等内源性因子可能参与胶质母细胞瘤的侵袭。

项目成果

Matsuno A: "Prolactin-secreting gangliocytoma:Letter to the editor"J Neurosurg. 95. 167-168 (2001)

松野 A：“泌乳素神经节细胞瘤：致编辑的信”J Neurosurg。

長島 正: "神経膠腫の成長因子"脳神経外科Advance Practice. 5. 164-167 (2002)

Tadashi Nagashima：“神经胶质瘤的生长因子”神经外科高级实践。5. 164-167 (2002)。

松野 彰: "ラット下垂体GHRHとSRIFの投与下でのRab3b蛋白の形態学的名発現様式とGH分泌との関連"ホルモンと臨床O2臨床増刊号内分泌病理学最近の進歩2002. 50. 41-44 (2002)

Akira Matsuno：“大鼠垂体 GHRH 和 SRIF 给药后 Rab3b 蛋白的形态表达模式及其与 GH 分泌的关系”激素与临床 O2 临床特刊内分泌病理学最新进展 2002. 50. 41-44 (2002)

Matsuno A: "Endocrinologic aspects of twenty-three patients with Rathke's cleft cyst"Endocrinologist. 11. 245-246 (2001)

松野 A：“23 名 Rathke 裂囊肿患者的内分泌方面”内分泌学家。

長島 正(分担): "脳神経外科 Advanced Practice 5神経膠腫"高倉公朋 メジカルビュー社. 190 (2002)

Tadashi Nagashima（撰稿人）：“神经外科高级实践 5 胶质瘤” Kimitomo Takakura Medical View Publishing 190（2002）。