Quantitative Study of Invasiveness of Malignant Gliomas

恶性胶质瘤侵袭性的定量研究

• 批准号: 02670623
• 负责人: NAGASHIMA Tadashi
• 金额: $ 1.34万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670623/
• 关键词: malignant glioma; invasion; cell-cell adhesion; N-cadherin; 回転培養; Nーカドヘリン; mーRNA; ラットグリオ-マ細胞株; グリア系株化腫瘍細胞; locomotion; 無血清培地; 細胞外マトリックス

At presnt, it is still difficult to cure patients with malignant glial tumors, mainly because of invasive growth into surrounding normal brain tissue. To clarify the invasion of glial tumors, we studied one of the cell adhesion molecules, N-cadherin, which is responsible for cell-cell adhesion in neural tissue. In this study, we investigated the correlation between expression of N-cadherin mRNA in rat glioma cell lines 9L,C6, RG12 and the extent of adhesion of these cells evaluated by in vitro cell aggregation assay. In Northern blot analysis, three different sizes of N-cadherin transcripts were identified in all three rat glioma cell lines, wheres only two sizes of bands were recognized in normal brain tissue. The degree of expression of these three bands varied amang each band size as well as each cell line. Especially, the degree of expression of the largest band (5.2Kb) varied considerably, strong in RG12, medium in 9L and weak in C6, and the degree of expression was related to the extent of adhesion of these cell lines estimated by in vitro cell aggregation assay. These results suggest that N-cadherin plays a role in cell-cell adhesion in glioma cells, and is involved in the invasion of glial tumors.

目前，恶性胶质瘤患者仍难以治愈，主要是因为其侵袭性生长至周围正常脑组织。为了阐明神经胶质瘤的侵袭，我们研究了一种细胞粘附分子 N-钙粘蛋白，它负责神经组织中的细胞间粘附。在本研究中，我们研究了大鼠神经胶质瘤细胞系9L、C6、RG12中N-钙粘蛋白mRNA的表达与通过体外细胞聚集测定评估的这些细胞的粘附程度之间的相关性。在 Northern 印迹分析中，在所有三种大鼠神经胶质瘤细胞系中均鉴定出三种不同大小的 N-钙粘蛋白转录本，而在正常脑组织中仅识别出两种大小的条带。这三个条带的表达程度因每个条带大小以及每个细胞系而异。特别是最大条带(5.2Kb)的表达程度差异很大，RG12强，9L中中，C6中弱，并且表达程度与体外细胞聚集测定估计的这些细胞系的粘附程度有关。这些结果表明N-钙粘蛋白在神经胶质瘤细胞的细胞粘附中发挥作用，并参与神经胶质瘤的侵袭。

项目成果

長島 正他: "転移性脳腫瘍" 診断と治療. 78. 2002-2006 (1990)

Masaru Nagashima 等：“转移性脑肿瘤”诊断和治疗。78。2002-2006（1990）。

長島 正,他: "脳腫瘍・テント上腫瘍" Clinical Neuroscience. 8. 983-986 (1990)

Tadashi Nagashima 等人：“脑肿瘤/幕上肿瘤”《临床神经科学》8. 983-986 (1990)。

長島 正 他: "転移性脳腫瘍" 診断と治療. 78. 2002-2006 (1990)

Tadashi Nagashima 等：“转移性脑肿瘤”诊断和治疗。78。2002-2006（1990）。

長島 正,他: "松果体の癌" 代謝. 27. 381-386 (1990)

Tadashi Nagashima 等人：“松果体癌”新陈代谢 27. 381-386 (1990)