Studies on invasive properties of malignant gliomas and development of treatment modalities for these tumors.

研究恶性胶质瘤的侵袭特性以及开发这些肿瘤的治疗方式。

• 批准号: 09671451
• 负责人: NAGASHIMA Tadashi
• 金额: $ 1.86万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671451/
• 关键词: invasion; glioma; in vivo; nude rat; 蛍光標識; 浸潤能; 免疫組織化学

5X10ィイD15ィエD1 fast blue-labeled U87MG or U251MG cells in 10μl of DMEM were injected into the right basal ganglia of 9-10weeks nude rat ( F344 / Njcl-rnu ). Two weeks after tumor implantation, the rat brains were fixed with 2% paraformaldehyde followed by 30% sucrose, embedded in OCT compound, freezing for cutting into 10μm thick sections with a cryostat. Sections were stained with hematoxylin and eosin, and observed under fluorescent microscope.The tumor cells invaded into the surrounding normal brain tissue and the scattered fast blue labeled-cells were observed around the solid tumor mass in U251MG tumor model, whereas the tumor cells were localized and the fast blue labeled- cells were not observed around the solid tumor mass in U87MG tumor model. The labeled-tumor cells which invaded into the narrow space of lateral ventricle were clearly distinguished in some cases. These results revealed that the brain tumor model using nude rat is useful to investigate the invasive properties of human glial tumors.

将5X10ィイD15ィエD1快蓝标记的U87MG或U251MG细胞于10μl DMEM中注射到9-10周裸鼠(F344/Njcl-rnu)的右侧基底节中。肿瘤植入两周后，将大鼠大脑用2%多聚甲醛固定，然后用30%蔗糖固定，包埋在OCT化合物中，冷冻后用低温恒温器切成10μm厚的切片。切片用苏木精和伊红染色，荧光显微镜下观察。U251MG肿瘤模型肿瘤细胞侵入周围正常脑组织，实体瘤块周围可见散在的固蓝标记细胞，而U87MG肿瘤模型肿瘤细胞呈局灶性，实体瘤块周围未见固蓝标记细胞。在某些情况下，侵入侧脑室狭窄空间的标记肿瘤细胞可以被清楚地区分。这些结果表明，使用裸鼠的脑肿瘤模型可用于研究人类神经胶质瘤的侵袭特性。

项目成果

Matsuno A, Ohsuga Y, Utsunomiya H, Taketoshi S, Munakata S, Nagao K, Osamura RY, Tamura A, Nagashima T: "An improved ultrastructural double staining method of rat growth hormone and its mRNA using LR White resin : a technical note"Histochem J. 30. 105-109

Matsuno A、Ohsuga Y、Utsunomiya H、Taketoshi S、Munakata S、Nagao K、Osamura RY、Tamura A、Nagashima T：“使用 LR White 树脂对大鼠生长激素及其 mRNA 进行改进的超微结构双重染色方法：技术说明”

Tanaka, S, Nagashima T, et al: "In vitro inhibition of binding of tumor necrosis factor (TNF)-2 by monoclonal untibody to INF receptor of glioma celland monocyte"Neurologia medico-chirurgica. 38. 812-818 (1998)

Tanaka, S, Nagashima T, et al:“单克隆抗体与神经胶质瘤细胞和单核细胞的 INF 受体结合的体外抑制肿瘤坏死因子 (TNF)-2 的结合”Neurologia medico-chirurgica。

Matsuno A et al: "An improved ultrastructural double-Staining method for rat growth hormone and its mRNA using White resin." Histochemical Journal. 30. 105-109 (1998)

Matsuno A 等人：“一种改进的使用白色树脂对大鼠生长激素及其 mRNA 进行超微结构双重染色的方法。”

Matsuno A et al: "Application of ultrastructural in site hybridization combined with immurohistochemistry to pothophysiological Studies." Acta Histochem.Cytochem.31. 259-265 (1998)

Matsuno A 等人：“超微结构原位杂交结合免疫组织化学在细胞生理学研究中的应用。”

Tanaka S et al: "In vitro Inhibition of binding of turnor necrosis factor[TNF]-2α by monoclonal antibody to TNF receptor on glioma cell and monocyte." Neurologia medico-chirurgica. 38. 812-818 (1998)

Tanaka S 等人：“神经胶质瘤细胞和单核细胞上的单克隆抗体对肿瘤坏死因子 [TNF]-2α 结合的体外抑制。” Neurologia medico-chirurgica。