Studies on invasive properties of malignant gliomas and development of treatment modalities for these tumors.

5X10-D15-D1 fast blue-labeled U87MG or U251MG cells in 10μl的DMEM were injected into the right basal ganglia of 9- 10 weeks nude rat （ F344 / Njcl-rnu ）。两周后，肿瘤植入后，大鼠大脑被固定在2%的超极性消除后被30%的蔗糖下，嵌入OCT复合物中，冻结用于切割10微米厚的部分与低温。用红细胞毒素和胰岛素存放的部分，并在荧光显微镜下观察到荧光显微镜下观察到的肿瘤细胞。肿瘤细胞进入了环绕正常脑组织和分散的快速蓝色标签--细胞在U251 MG肿瘤模型中观察到的固体肿瘤质量，在那里肿瘤细胞被本地化和快速蓝色标签--细胞在U87 MG肿瘤模型中不观察到固体肿瘤质量。标签上的肿瘤细胞被植入了一个狭窄的空间，进入了一些案例中的口腔肿瘤细胞。这些结果揭示了使用裸鼠的大脑肿瘤模型的大脑肿瘤模型是有用的，可以用来调查人类胶质肿瘤的入侵性质。

5X10ィイD15ィエD1 fast blue-labeled U87MG or U251MG cells in 10μl of DMEM were injected into the right basal ganglia of 9-10weeks nude rat ( F344 / Njcl-rnu ). Two weeks after tumor implantation, the rat brains were fixed with 2% paraformaldehyde followed by 30% sucrose, embedded in OCT compound, freezing for cutting into 10μm thick sections with a cryostat. Sections were stained with hematoxylin and eosin, and observed under fluorescent microscope.The tumor cells invaded into the surrounding normal brain tissue and the scattered fast blue labeled-cells were observed around the solid tumor mass in U251MG tumor model, whereas the tumor cells were localized and the fast blue labeled- cells were not observed around the solid tumor mass in U87MG tumor model. The labeled-tumor cells which invaded into the narrow space of lateral ventricle were clearly distinguished in some cases. These results revealed that the brain tumor model using nude rat is useful to investigate the invasive properties of human glial tumors.