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Studies on invasive properties of malignant gliomas and development of treatment modalities for these tumors.

Studies on invasive properties of malignant gliomas and development of treatment modalities for these tumors.
研究恶性胶质瘤的侵袭特性以及开发这些肿瘤的治疗方式。
批准号:
09671451
负责人:
NAGASHIMA Tadashi
金额:
$1.86万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998

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项目成果

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中文摘要
翻译
5x10 - D15 - D1 fast blue-labeled U87MG或U251MG cells in 10μl of DMEM were injected into the rightbasal ganglia of 9-10weeks nude rat (F344 / Njcl-rnu). Two weeks after tumor implantation,rat brains fixed with 2% paraformaldehyde followed by 30% sucrose,embedded in OCT compound,freezing for cutting into 10μm thick sections with a cryostat. sections were stained withhematoxylin and eosin,observed under fluorescent microscopee . the tumor cells invaded into the surrounding normal braintissue and the scattered fast blue labeled-cells were observed around the solid tumor mass in U251MGtumor model,whereas the tumor cells were localized and the fast blue labeled- cells not observed around thesolid tumor mass in U87MG tumor model. The labeled-tumor cells which invaded into The narrow spacelateral ventricle clearly distinguished in some cases.这些results revealed that the braintumor model using nude rat is useful to investigate the invasive properties of human glial tumors
英文摘要
5X10ィイD15ィエD1 fast blue-labeled U87MG or U251MG cells in 10μl of DMEM were injected into the right basal ganglia of 9-10weeks nude rat ( F344 / Njcl-rnu ). Two weeks after tumor implantation, the rat brains were fixed with 2% paraformaldehyde followed by 30% sucrose, embedded in OCT compound, freezing for cutting into 10μm thick sections with a cryostat. Sections were stained with hematoxylin and eosin, and observed under fluorescent microscope.The tumor cells invaded into the surrounding normal brain tissue and the scattered fast blue labeled-cells were observed around the solid tumor mass in U251MG tumor model, whereas the tumor cells were localized and the fast blue labeled- cells were not observed around the solid tumor mass in U87MG tumor model. The labeled-tumor cells which invaded into the narrow space of lateral ventricle were clearly distinguished in some cases. These results revealed that the brain tumor model using nude rat is useful to investigate the invasive properties of human glial tumors.
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会议论文
Matsuno A, Ohsuga Y, Utsunomiya H, Taketoshi S, Munakata S, Nagao K, Osamura RY, Tamura A, Nagashima T: "An improved ultrastructural double staining method of rat growth hormone and its mRNA using LR White resin : a technical note"Histochem J. 30. 105-109
Matsuno A、Ohsuga Y、Utsunomiya H、Taketoshi S、Munakata S、Nagao K、Osamura RY、Tamura A、Nagashima T:“使用 LR White 树脂对大鼠生长激素及其 mRNA 进行改进的超微结构双重染色方法:技术说明”
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作者: []
通讯作者:
Tanaka, S, Nagashima T, et al: "In vitro inhibition of binding of tumor necrosis factor (TNF)-2 by monoclonal untibody to INF receptor of glioma celland monocyte"Neurologia medico-chirurgica. 38. 812-818 (1998)
Tanaka, S, Nagashima T, et al:“单克隆抗体与神经胶质瘤细胞和单核细胞的 INF 受体结合的体外抑制肿瘤坏死因子 (TNF)-2 的结合”Neurologia medico-chirurgica。
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通讯作者:
Matsuno A et al: "An improved ultrastructural double-Staining method for rat growth hormone and its mRNA using White resin." Histochemical Journal. 30. 105-109 (1998)
Matsuno A 等人:“一种改进的使用白色树脂对大鼠生长激素及其 mRNA 进行超微结构双重染色的方法。”
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作者: []
通讯作者:
Tanaka S et al: "In vitro Inhibition of binding of turnor necrosis factor[TNF]-2α by monoclonal antibody to TNF receptor on glioma cell and monocyte." Neurologia medico-chirurgica. 38. 812-818 (1998)
Tanaka S 等人:“神经胶质瘤细胞和单核细胞上的单克隆抗体对肿瘤坏死因子 [TNF]-2α 结合的体外抑制。” Neurologia medico-chirurgica。
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通讯作者:
23
    Studies on the invasive potential of malignant gliomas
    • 批准号:
      13671469
    • 项目类别:
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    • 资助金额:
      $1.6万
    • 财政年份:
      2001
    • 负责人:
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    • 依托单位:
    Influence of Inserting Bite Plane on the Position of Mandibular Condyle
    • 批准号:
      11470414
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $7.68万
    • 财政年份:
      1999
    • 负责人:
      NAGASHIMA Tadashi
    • 依托单位:
    Study on the practical application of chemo-radiotherapy for malignant brain tumor
    • 批准号:
      11671393
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.98万
    • 财政年份:
      1999
    • 负责人:
      NAGASHIMA Tadashi
    • 依托单位:
    Quantitative Study of Invasiveness of Malignant Gliomas
    • 批准号:
      02670623
    • 项目类别:
      Grant-in-Aid for General Scientific Research (C)
    • 资助金额:
      $1.34万
    • 财政年份:
      1990
    • 负责人:
      NAGASHIMA Tadashi
    • 依托单位:
    国内基金
    海外基金
    双模式编码的慢病毒载体转染C6 Glioma Cells的影像学研究
    • 批准号:
      81271563
    • 项目类别:
      面上项目
    • 资助金额:
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    • 批准年份:
      2012
    • 负责人:
      陈正光
    • 依托单位:
    胶质瘤中miR-93的转录调控及其促细胞周期进展的新机制
    • 批准号:
      81101915
    • 项目类别:
      青年科学基金项目
    • 资助金额:
      23.0万元
    • 批准年份:
      2011
    • 负责人:
      张安玲
    • 依托单位:
    解析miR-566调控VHL/β-catenin信号通路影响人脑胶质瘤血管新生的分子机制
    • 批准号:
      81101916
    • 项目类别:
      青年科学基金项目
    • 资助金额:
      22.0万元
    • 批准年份:
      2011
    • 负责人:
      周旋
    • 依托单位:
    胶质瘤中miR-23b介导ICAT负调控β-catenin/TCF4信号通路的新机制
    • 批准号:
      81001128
    • 项目类别:
      青年科学基金项目
    • 资助金额:
      22.0万元
    • 批准年份:
      2010
    • 负责人:
      韩磊
    • 依托单位: